Abstract
Objectives: The species Silybum marianum (L.) (Asteraceae) commonly known as Milk thistle is medicinally valuable and due to over exploitation the species may become threatened. Hence, we have made an attempt to optimize the protocol for its direct regeneration through cotyledon explants using in vitro culture technology. Methods/Statistical Analysis: For in vitro multiple-shoot induction, cotyledon explants were excised from surfacesterilized seeds and cultured on MS Basal Medium (MBM) enriched with diverse conc. (10.0–20.0 µM) of BAP/KIN alone and also 10.0 µM BAP/KIN in combination with 1.25–5.0 µM NAA/IAA/IBA. The micro-shoots were cut out and cultured on 1/2 strength MBM enriched with diverse concentrations (5.0-20.0 µM) of NAA/IAA/IBA alone and also coupled with 1.25–5.0 µM BAP/KIN. Findings: In this study, superlative rate of shoot induction, maximal Number of Shoots Per Explant (NSPE) and longest shoot-length were achieved on MBM enriched with 10.0 μM BAP + 2.5 μM NAA. Superlative rate of root induction, mean number of roots per shoot and mean root length were documented on MBM supplemented with 15.0 μM NAA + 2.5 μM BAP. Application/Improvement: Being over exploited, the current technique can be operated for accelerated multiplication and conservation of the species. Further, this protocol can also be operated for transfer of novel genes for enhancement and modulation of bioactive molecules production in S. marianum – a medicinally valuable plant. Keywords: Cotyledon, In vitro Regeneration, Silybum Marianum
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Claims 4
Claim · model-written summary
MBM with 10.0 μM BAP and 2.5 μM NAA gave the best shoot induction rate, shoot number and shoot length.
- Taxon
- Silybum marianum (L.)
Silybum marianume1 - Treatment
- MBM enriched with 10.0 μM BAP + 2.5 μM NAAe0
- Outcome
- rate of shoot induction, maximal Number of Shoots Per Explant (NSPE) and longest shoot-lengthe0
- Direction
- superlativee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“superlative rate of shoot induction, maximal Number of Shoots Per Explant (NSPE) and longest shoot-length were achieved on MBM enriched with 10.0 μM BAP + 2.5 μM NAA”
e1“The species Silybum marianum (L.)”
Claim · model-written summary
MBM with 15.0 μM NAA and 2.5 μM BAP gave the best root induction rate, root number and root length.
- Taxon
- Silybum marianum (L.)Silybum marianume1
- Treatment
- MBM supplemented with 15.0 μM NAA + 2.5 μM BAPe0
- Outcome
- rate of root induction, mean number of roots per shoot and mean root lengthe0
- Direction
- Superlativee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Superlative rate of root induction, mean number of roots per shoot and mean root length were documented on MBM supplemented with 15.0 μM NAA + 2.5 μM BAP”
e1“The species Silybum marianum (L.)”
Claim · model-written summary
The authors propose the regeneration technique for multiplication and conservation.
- Taxon
- Silybum marianum (L.)Silybum marianume1
- Outcome
- accelerated multiplication and conservatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the current technique can be operated for accelerated multiplication and conservation of the species”
e1“The species Silybum marianum (L.)”
Claim · model-written summary
The authors propose the protocol for transferring novel genes to modify bioactive molecule production.
- Taxon
- S. marianumSilybum marianume0
- Outcome
- transfer of novel genes for enhancement and modulation of bioactive molecules productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this protocol can also be operated for transfer of novel genes for enhancement and modulation of bioactive molecules production in S. marianum”
The same questions in other papers
in vitro · growth · Silybum marianum (milk thistle)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.