Abstract
Cadmium-induced glycine-rich protein (cdiGRP) is a cell wall-associated factor that increases callose levels in plant vasculature. To better understand the cdiGRP/callose regulation system, we identified a tobacco protein, GrIP (cdiGRP-interacting protein, GrIP), that associates with cdiGRP and localizes at the plant cell wall. Constitutive overexpression of GrIP enhanced the accumulation of the cdiGRP protein and callose in vasculature-associated cells with or without treatment with cadmium ions. That GrIP gene expression was not affected by cadmium ions indicated that GrIP does not directly modulate the callose levels induced by the treatment. Instead, GrIP most likely functions by further elevating the accumulated amount of cdiGRP, the expression of which is up-regulated by the cadmium ions. Interestingly, the levels of cdiGRP mRNA were not affected by constitutive expression of GrIP, demonstrating that the enhancement in cdiGRP protein accumulation by GrIP overexpression occurs posttranslationally. Collectively, these observations suggest that GrIP interacts with cdiGRP and increases its level of accumulation; in turn, the elevated amounts of cdiGRP induce callose deposits in the plant cell walls. Therefore, GrIP and cdiGRP represent sequentially acting factors in a biochemical pathway that regulates callose accumulation in the plant vasculature.
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Claims 6
Claim · model-written summary
A tobacco protein, GrIP, was identified that associates with cdiGRP and localizes at the plant cell wall.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- GrIPe0
- Outcome
- associates with cdiGRP and localizes at the plant cell walle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein, GrIP (cdiGRP-interacting protein, GrIP), that associates with cdiGRP and localizes at the plant cell wall”
Claim · model-written summary
Constitutive overexpression of GrIP enhanced the accumulation of cdiGRP protein and callose in vasculature-associated cells, with or without cadmium ion treatment.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- vasculature-associated cellse1
- Treatment
- Constitutive overexpression of GrIPe1
- Gene
- GrIPe1
- Outcome
- accumulation of the cdiGRP protein and callosee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein”
e1“Constitutive overexpression of GrIP enhanced the accumulation of the cdiGRP protein and callose in vasculature-associated cells with or without treatment with cadmium ions”
Claim · model-written summary
GrIP gene expression was not affected by cadmium ions.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- cadmium ionse1
- Gene
- GrIPe1
- Outcome
- GrIP gene expressione1
- Direction
- not affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein”
e1“That GrIP gene expression was not affected by cadmium ions”
Claim · model-written summary
cdiGRP mRNA levels were not affected by constitutive expression of GrIP.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- constitutive expression of GrIPe1
- Gene
- cdiGRPe1
- Outcome
- levels of cdiGRP mRNAe1
- Direction
- not affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein”
e1“the levels of cdiGRP mRNA were not affected by constitutive expression of GrIP”
Claim · model-written summary
The authors conclude that the enhancement of cdiGRP protein accumulation by GrIP overexpression occurs posttranslationally.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- GrIPe1
- Outcome
- enhancement in cdiGRP protein accumulatione1
- Qualifier
- occurs posttranslationallye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein”
e1“demonstrating that the enhancement in cdiGRP protein accumulation by GrIP overexpression occurs posttranslationally”
Claim · model-written summary
The authors suggest that GrIP interacts with cdiGRP and increases its accumulation, and that the elevated cdiGRP in turn induces callose deposits in the cell wall.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- GrIPe1
- Outcome
- level of accumulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a tobacco protein”
e1“GrIP interacts with cdiGRP and increases its level of accumulation”
The same questions in other papers
cadmium atom · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
1 paper more in this group
See all 5 papers →overexpression · quality: protein · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.