Abstract
BACKGROUND: Arginine and citrulline serve as nitrogen storage forms, but are also involved in biosynthetic and catabolic pathways. Metabolism of arginine, citrulline and ornithine is distributed between mitochondria and cytosol. For the shuttle of intermediates between cytosol and mitochondria transporters present on the inner mitochondrial membrane are required. Yeast contains a mitochondrial translocator for ornithine and arginine, Ort1p/Arg11p. Ort1p/Arg11p is a member of the mitochondrial carrier family (MCF) essential for ornithine export from mitochondria. The yeast arg11 mutant, which is deficient in Ort1p/Arg11p grows poorly on media lacking arginine. RESULTS: High-level expression of a nuclear encoded Arabidopsis thaliana homolog (AtmBAC2) of Ort1p/Arg11p was able to suppress the growth deficiency of arg11. RT-PCR analysis demonstrated expression of AtmBAC2 in all tissues with highest levels in flowers. Promoter-GUS fusions showed preferential expression in flowers, i.e. pollen, in the vasculature of siliques and in aborted seeds. Variable expression was observed in leaf vasculature. Induction of the promoter was not observed during the first two weeks in seedlings grown on media containing NH4NO3, arginine or ornithine as sole nitrogen sources. CONCLUSION: AtmBAC2 was isolated as a mitochondrial transporter for arginine in Arabidopsis. The absence of expression in developing seeds and in cotyledons of seedlings indicates that other transporters are responsible for storage and mobilization of arginine in seeds.
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Claims 6
Claim · model-written summary
High-level expression of AtmBAC2 suppressed the growth deficiency of yeast arg11 mutant.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Material
- arg11e1
- Treatment
- High-level expressione1
- Gene
- AtmBAC2e1
- Outcome
- growth deficiency of arg11e1
- Direction
- suppresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“High-level expression of a nuclear encoded Arabidopsis thaliana homolog (AtmBAC2) of Ort1p/Arg11p was able to suppress the growth deficiency of arg11”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
Claim · model-written summary
AtmBAC2 expression was detected in all tissues, highest in flowers.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- flowerse2
- Gene
- AtmBAC2e2
- Outcome
- expression of AtmBAC2e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“RT-PCR analysis demonstrated expression of AtmBAC2 in all tissues with highest levels in flowers”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
Claim · model-written summary
Promoter-GUS fusions showed preferential AtmBAC2 expression in flowers, pollen, siliques vasculature and aborted seeds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- vasculature of siliquese3
- Outcome
- preferential expressione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Promoter-GUS fusions showed preferential expression in flowers, i.e. pollen, in the vasculature of siliques and in aborted seeds”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
Claim · model-written summary
AtmBAC2 promoter activity was variable in leaf vasculature.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leaf vasculaturee4
- Outcome
- Variable expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Variable expression was observed in leaf vasculature”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
Claim · model-written summary
The AtmBAC2 promoter was not induced in seedlings grown on NH4NO3, arginine or ornithine as sole nitrogen source during the first two weeks.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seedlingse5
- Treatment
- NH4NO3, arginine or ornithine as sole nitrogen sourcese5
- Outcome
- Induction of the promotere5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Induction of the promoter was not observed during the first two weeks in seedlings grown on media containing NH4NO3, arginine or ornithine as sole nitrogen sources”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
Claim · model-written summary
AtmBAC2 expression was absent in developing seeds and cotyledons of seedlings.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- developing seedse6
- Outcome
- absence of expressione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The absence of expression in developing seeds and in cotyledons of seedlings”
t0“Expression pattern of a nuclear encoded mitochondrial arginine-ornithine translocator gene from Arabidopsis”
The same questions in other papers
nitrogen · molecular: expression · ArabidopsisPositive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.