Abstract
The maize dull1 (du1) gene is a determinant of the structure of endosperm starch, and du1- mutations affect the activity of two enzymes involved in starch biosynthesis, starch synthase II (SSII) and starch branching enzyme IIa (SBEIIa). Six novel du1- mutations generated in Mutator-active plants were identified. A portion of the du1 locus was cloned by transposon tagging, and a nearly full-length Du1 cDNA sequence was determined. Du1 codes for a predicted 1674-residue protein, comprising one portion that is similar to SSIII of potato, as well as a large unique region. Du1 transcripts are present in the endosperm during the time of starch biosynthesis, but the mRNA was undetectable in leaf or root tissue. The predicted size of the Du1 gene product and its expression pattern are consistent with those of maize SSII. The Du1 gene product contains two repeated regions in its unique N terminus. One of these contains a sequence identical to a conserved segment of SBEs. We conclude that Du1 codes for a starch synthase, most likely SSII, and that secondary effects of du1- mutations, such as reduction of SBEIIa, result from the primary deficiency in this starch synthase.
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Claims 13
Claim · model-written summary
Six novel du1- mutations generated in Mutator-active maize plants were identified.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e1“Six novel du1- mutations generated in Mutator-active plants were identified”
Claim · model-written summary
A nearly full-length Du1 cDNA sequence was determined in maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e2“A portion of the du1 locus was cloned by transposon tagging, and a nearly full-length Du1 cDNA sequence was determined”
Claim · model-written summary
A portion of the du1 locus was cloned by transposon tagging in maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e2“A portion of the du1 locus was cloned by transposon tagging, and a nearly full-length Du1 cDNA sequence was determined”
Claim · model-written summary
The Du1 gene codes for a predicted 1674-residue protein.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e3“Du1 codes for a predicted 1674-residue protein, comprising one portion that is similar to SSIII of potato, as well as a large unique region”
Claim · model-written summary
The predicted Du1 protein portion is similar to SSIII of potato.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e3“Du1 codes for a predicted 1674-residue protein, comprising one portion that is similar to SSIII of potato, as well as a large unique region”
Claim · model-written summary
Du1 transcripts are present in maize endosperm during starch biosynthesis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e4“Du1 transcripts are present in the endosperm during the time of starch biosynthesis, but the mRNA was undetectable in leaf or root tissue”
Claim · model-written summary
The mRNA of Du1 was undetectable in maize leaf or root tissue.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e4“Du1 transcripts are present in the endosperm during the time of starch biosynthesis, but the mRNA was undetectable in leaf or root tissue”
Claim · model-written summary
The predicted size and expression pattern of the Du1 gene product are consistent with those of maize SSII.
- Gene
- SSIIe5
- Outcome
- predicted size of the Du1 gene product and its expression patterne5
- Qualifier
- consistent with those of maize SSIIe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e5“The predicted size of the Du1 gene product and its expression pattern are consistent with those of maize SSII”
Claim · model-written summary
The Du1 gene product contains two repeated regions in its unique N terminus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e6“The Du1 gene product contains two repeated regions in its unique N terminus”
Claim · model-written summary
One Du1 repeated region contains a sequence identical to a conserved segment of SBEs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e7“One of these contains a sequence identical to a conserved segment of SBEs”
Claim · model-written summary
Du1 codes for a starch synthase, most likely SSII.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e7“We conclude that Du1 codes for a starch synthase, most likely SSII”
Claim · model-written summary
Secondary reduction of SBEIIa in du1- mutants results from the primary deficiency in starch synthase.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e6“secondary effects of du1- mutations, such as reduction of SBEIIa, result from the primary deficiency in this starch synthase”
Claim · model-written summary
du1- mutations affect the activity of two starch biosynthesis enzymes, starch synthase II (SSII) and starch branching enzyme IIa (SBEIIa).
- Treatment
- du1- mutationse5
- Gene
- starch synthase II (SSII)e5
- Outcome
- activity of two enzymes involved in starch biosynthesise5
- Qualifier
- starch branching enzyme IIa (SBEIIa)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maize dull1 (du1) gene is a determinant of the structure of endosperm starch”
e5“du1- mutations affect the activity of two enzymes involved in starch biosynthesis, starch synthase II (SSII) and starch branching enzyme IIa (SBEIIa)”