Abstract
WRKY transcription factors play a central role in transcriptional reprogramming associated with plant immune responses. However, due to functional redundancy, typically the contribution of individual members of this family to immunity is only subtle. Using microarray analysis, we found that the paralogous tomato WRKY genes SlWRKY72a and b are transcriptionally up-regulated during disease resistance mediated by the R gene Mi-1. Virus-induced gene silencing of these two genes in tomato resulted in a clear reduction of Mi-1-mediated resistance as well as basal defense against root-knot nematodes (RKN) and potato aphids. Using Arabidopsis T-DNA insertion mutants, we found that their Arabidopsis ortholog, AtWRKY72, is also required for full basal defense against RKN as well as to the oomycete Hyaloperonospora arabidopsidis. Despite their similar roles in basal defense against RKN in both tested plant species, WRKY72-type transcription factors in tomato, but not in Arabidopsis, clearly contributed to basal defense against the bacterial pathogen Pseudomonas syringae. Of the five R genes that we tested in tomato and Arabidopsis, only Mi-1 appeared to be dependent on WRKY72-type transcription factors. Interestingly, AtWRKY72 target genes, identified by microarray analysis of H. arabidopsidis-triggered transcriptional changes, appear to be largely non-responsive to analogs of the defense hormone salicylic acid (SA). Thus, similarly to Mi-1, which in part acts independently of SA, AtWRKY72 appears to utilize SA-independent defense mechanisms. We propose that WRKY72-type transcription factors play a partially conserved role in basal defense in tomato and Arabidopsis, a function that has been recruited to serve Mi-1-dependent immunity.
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Claims 12
Claim · model-written summary
Tomato SlWRKY72a and b were upregulated during Mi-1-mediated resistance.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- SlWRKY72a and be0
- Outcome
- transcriptionally up-regulatede0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the paralogous tomato WRKY genes SlWRKY72a and b are transcriptionally up-regulated during disease resistance mediated by the R gene Mi-1”
Claim · model-written summary
Silencing SlWRKY72a and b reduced Mi-1-mediated resistance in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Virus-induced gene silencinge0
- Gene
- these two genese0
- Outcome
- Mi-1-mediated resistancee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing of these two genes in tomato resulted in a clear reduction of Mi-1-mediated resistance as well as basal defense against root-knot nematodes (RKN) and potato aphids”
Claim · model-written summary
Silencing the two genes reduced tomato basal defense against root-knot nematodes.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Virus-induced gene silencinge0
- Interactor
- root-knot nematodes
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogynee0pest - Outcome
- basal defense against root-knot nematodese0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“Virus-induced gene silencing of these two genes in tomato resulted in a clear reduction of Mi-1-mediated resistance as well as basal defense against root-knot nematodes (RKN) and potato aphids”
Claim · model-written summary
Silencing the two genes reduced tomato basal defense against potato aphids.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Virus-induced gene silencinge0
- Interactor
- potato aphidse0pest
- Outcome
- basal defense against root-knot nematodes (RKN) and potato aphidse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing of these two genes in tomato resulted in a clear reduction of Mi-1-mediated resistance as well as basal defense against root-knot nematodes (RKN) and potato aphids”
Claim · model-written summary
AtWRKY72 was required for full Arabidopsis defense against root-knot nematodes.
- Taxon
- Arabidopsis
Arabidopsise0 - Interactor
- RKNe0pest
- Gene
- AtWRKY72e0
- Outcome
- full basal defense against RKNe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their Arabidopsis ortholog, AtWRKY72, is also required for full basal defense against RKN as well as to the oomycete Hyaloperonospora arabidopsidis”
Claim · model-written summary
AtWRKY72 was required for full Arabidopsis defense against Hyaloperonospora arabidopsidis.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- Hyaloperonospora arabidopsidis
Hyaloperonospora arabidopsidise0pathogen - Gene
- AtWRKY72e0
- Outcome
- full basal defense against RKN as well as to the oomycete Hyaloperonospora arabidopsidise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their Arabidopsis ortholog, AtWRKY72, is also required for full basal defense against RKN as well as to the oomycete Hyaloperonospora arabidopsidis”
Claim · model-written summary
WRKY72 factors contributed to tomato basal defense against Pseudomonas syringae.
- Taxon
- tomatoSolanum lycopersicume0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Gene
- WRKY72-type transcription factorse0
- Outcome
- basal defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY72-type transcription factors in tomato, but not in Arabidopsis, clearly contributed to basal defense against the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
WRKY72 factors did not contribute to Arabidopsis basal defense against Pseudomonas syringae.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- Pseudomonas syringaePseudomonas syringaee0pathogen
- Gene
- WRKY72-type transcription factorse0
- Outcome
- basal defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY72-type transcription factors in tomato, but not in Arabidopsis, clearly contributed to basal defense against the bacterial pathogen Pseudomonas syringae”
Claim · model-written summary
Among five tested R genes, only Mi-1 depended on WRKY72 factors.
- Taxon
- tomato and ArabidopsisArabidopsisSolanum lycopersicume0
- Gene
- Mi-1e0
- Outcome
- dependent on WRKY72-type transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of the five R genes that we tested in tomato and Arabidopsis, only Mi-1 appeared to be dependent on WRKY72-type transcription factors”
Claim · model-written summary
AtWRKY72 targets were largely unresponsive to salicylic acid analogs.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- analogs of the defense hormone salicylic acid (SA)e0
- Interactor
- H. arabidopsidisHyaloperonospora arabidopsidise0pathogen
- Gene
- AtWRKY72 target genese0
- Outcome
- non-responsivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtWRKY72 target genes, identified by microarray analysis of H. arabidopsidis-triggered transcriptional changes, appear to be largely non-responsive to analogs of the defense hormone salicylic acid (SA)”
t0“Arabidopsis”
Claim · model-written summary
The authors infer that AtWRKY72 uses salicylic acid independent defense.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtWRKY72e0
- Outcome
- SA-independent defense mechanismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtWRKY72 appears to utilize SA-independent defense mechanisms”
t0“Arabidopsis”
Claim · model-written summary
The authors propose a partially conserved WRKY72 basal defense role recruited for Mi-1 immunity.
- Taxon
- tomato and ArabidopsisArabidopsisSolanum lycopersicume0
- Gene
- WRKY72-type transcription factorse0
- Outcome
- Mi-1-dependent immunitye0
- Qualifier
- partially conserved role in basal defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY72-type transcription factors play a partially conserved role in basal defense in tomato and Arabidopsis, a function that has been recruited to serve Mi-1-dependent immunity”
The same questions in other papers
salicylic acid · Hyaloperonospora arabidopsidis · ArabidopsisResults agree
1 paper in the same direction as this paper
- negative
loss of function · Meloidogyne · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
loss of function · disease · Solanum lycopersicum (tomato)Positive and negative results
62 papers in the same direction as this paper · 92 in the opposite direction
- negative
- no effect
- no effect
151 papers more in this group
See all 155 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.