Abstract
In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A members. To gain insight into the conservation of ABI1 function in Brassicaceae, and understand better its regulatory effects in the drought stress response, we generated transgenic B. napus plants overexpressing A. thaliana ABI1. Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants. We present the characterization of the drought response of B. napus with the participation of ABI1-like paralogues. The expression pattern of two evolutionarily distant paralogues, BnaA01.ABI1.a and BnaC07.ABI1.b in B. napus and their promoter activity in A. thaliana showed differences in the induction of the paralogues under dehydration stress. Comparative sequence analysis of both BnaABI1 promoters showed variation in positions of cis-acting elements that are especially important for ABA- and stress-inducible expression. Together, these data reveal that subfunctionalization following gene duplication may be important in the maintenance and functional divergence of the BnaABI1 paralogues. Our results provide a framework for a better understanding of (1) the role of ABI1 as a hub protein regulator of the drought response, and (2) the differential involvement of the duplicated BnaABI1 genes in the response of B. napus to dehydration-related stresses.
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Claims 7
Claim · model-written summary
Six B. napus sequences were identified as putative PP2C group A members.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Gene
- PP2C group Ae0
- Outcome
- Six homologous B. napus sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Brassica napus L.”
e0“Six homologous B. napus sequences were identified and characterized as putative PP2C group A members.”
Claim · model-written summary
ABI1 overexpression reduced relative water content under drought compared with wild type.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- overexpressing A. thaliana ABI1e1
- Gene
- A. thaliana ABI1e1
- Outcome
- relative water contente0
- Direction
- decreasee0
- Qualifier
- droughte0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants.”
e1“overexpressing A. thaliana ABI1”
Claim · model-written summary
ABI1 overexpression reduced photosynthetic pigment content under drought compared with wild type.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- overexpressing A. thaliana ABI1e1
- Outcome
- photosynthetic pigments contente0
- Direction
- decreasee0
- Qualifier
- droughte0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants.”
e1“overexpressing A. thaliana ABI1”
Claim · model-written summary
ABI1 overexpression reduced RAB18 and RD19A marker expression under drought.
- Taxon
- Brassica napus L.Brassica napust0
- Treatment
- overexpressing A. thaliana ABI1e1
- Outcome
- expression level of RAB18- and RD19A-drought-responsive marker genese0
- Direction
- decreasee0
- Qualifier
- droughte0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants.”
e1“overexpressing A. thaliana ABI1”
Claim · model-written summary
BnaA01.ABI1.a and BnaC07.ABI1.b differed in dehydration induction.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- BnaA01.ABI1.a and BnaC07.ABI1.be0
- Outcome
- differences in the induction of the paraloguese0
- Qualifier
- dehydration stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“The expression pattern of two evolutionarily distant paralogues, BnaA01.ABI1.a and BnaC07.ABI1.b in B. napus and their promoter activity in A. thaliana showed differences in the induction of the paralogues under dehydration stress.”
Claim · model-written summary
The two BnaABI1 promoters varied in positions of stress and ABA response elements.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- BnaABI1e0
- Outcome
- variation in positions of cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“Comparative sequence analysis of both BnaABI1 promoters showed variation in positions of cis-acting elements that are especially important for ABA- and stress-inducible expression.”
Claim · model-written summary
Gene duplication subfunctionalization may maintain and diversify BnaABI1 paralogues.
- Taxon
- Brassica napus L.Brassica napust0
- Gene
- BnaABI1e0
- Outcome
- maintenance and functional divergencee0
- Qualifier
- subfunctionalization following gene duplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus L.”
e0“subfunctionalization following gene duplication may be important in the maintenance and functional divergence of the BnaABI1 paralogues”