Abstract
Using pharmacological and biochemical approaches, the role of maize polyamine oxidase (MPAO) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays L.) plants was investigated. Exogenous ABA treatment enhanced the expression of the MPAO gene and the activities of apoplastic MPAO. Pretreatment with two different inhibitors for apoplastic MPAO partly reduced hydrogen peroxide (H2O2) accumulation induced by ABA and blocked the ABA-induced expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymes. Treatment with spermidine, the optimum substrate of MPAO, also induced the expression and the activities of the antioxidant enzymes, and the upregulation of the antioxidant enzymes was prevented by two inhibitors of MPAO and two scavengers of H2O2. These results suggest that MPAO contributes to ABA-induced cytosolic antioxidant defense through H2O2, a Spd catabolic product.
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Claims 6
Claim · model-written summary
ABA enhanced MPAO gene expression and apoplastic MPAO activity.
- Treatment
- Exogenous ABA treatmente0
- Gene
- MPAOe0
- Outcome
- expression of the MPAO gene and the activities of apoplastic MPAOe0
- Direction
- enhancede0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous ABA treatment enhanced the expression of the MPAO gene and the activities of apoplastic MPAO.”
t0“Leaves of Maize”
Claim · model-written summary
MPAO inhibitors partly reduced ABA-induced hydrogen peroxide accumulation.
- Treatment
- two different inhibitors for apoplastic MPAOe0
- Gene
- MPAOe0
- Outcome
- hydrogen peroxide (H2O2) accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pretreatment with two different inhibitors for apoplastic MPAO partly reduced hydrogen peroxide (H2O2) accumulation induced by ABA”
t0“Leaves of Maize”
Claim · model-written summary
MPAO inhibitors blocked ABA-induced antioxidant gene expression and cytosolic antioxidant enzyme activity.
- Outcome
- expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymese0
- Direction
- blockede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blocked the ABA-induced expression of the antioxidant genes superoxide dismutase 4 and cytosolic ascorbate peroxidase and the activities of the cytosolic antioxidant enzymes”
t0“Leaves of Maize”
Claim · model-written summary
Spermidine induced antioxidant enzyme expression and activity.
- Treatment
- Treatment with spermidinee0
- Outcome
- expression and the activities of the antioxidant enzymese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment with spermidine, the optimum substrate of MPAO, also induced the expression and the activities of the antioxidant enzymes”
t0“Leaves of Maize”
Claim · model-written summary
MPAO inhibitors and hydrogen peroxide scavengers prevented antioxidant enzyme upregulation.
- Treatment
- two inhibitors of MPAO and two scavengers of H2O2e0
- Outcome
- upregulation of the antioxidant enzymese0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the upregulation of the antioxidant enzymes was prevented by two inhibitors of MPAO and two scavengers of H2O2”
t0“Leaves of Maize”
Claim · model-written summary
MPAO may contribute to ABA-induced cytosolic antioxidant defence through hydrogen peroxide.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MPAO contributes to ABA-induced cytosolic antioxidant defense through H2O2, a Spd catabolic product”
t0“Leaves of Maize”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.