Abstract
We report the characterization of rice OsHKT1 (Oryza sativa ssp. indica) homologous to the wheat K+/Na+-symporter HKT1. Expression of OsHKT1 in the yeast strain CY162 defective in K+-uptake restored growth at mM and micro M concentrations of K+ and mediated hypersensitivity to Na+. When expressed in Xenopus oocytes, rice OsHKT1 showed uptake characteristics of a Na+-transporter but mediated transport of other alkali cations as well. OsHKT1 expression was analysed in salt-tolerant rice Pokkali and salt-sensitive IR29 in response to external cation concentrations. OsHKT1 is expressed in roots and leaves. Exposure to Na+, Rb+, Li+, and Cs+ reduced OsHKT1 transcript amounts in both varieties and, in some cases, incompletely spliced transcripts were observed. By in situ hybridizations the expression of OsHKT1 was localized to the root epidermis and the vascular tissue inside the endodermis. In leaves, OsHKT1 showed strongest signals in cells surrounding the vasculature. The repression of OsHKT1 in the two rice varieties during salt stress was different in various cell types with main differences in the root vascular tissue. The data suggest control over HKT expression as a factor that may distinguish salt stress-sensitive and stress-tolerant lines. Differences in transcript expression in space and time in different lines of the same species appear to be a component of ion homeostasis correlated with salt sensitivity and tolerance.
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Claims 9
Claim · model-written summary
OsHKT1 restored yeast growth at low K+ concentrations.
- Taxon
- rice
Oryza sativat0 - Treatment
- Expression of OsHKT1e0
- Gene
- OsHKT1e0
- Outcome
- growthe0
- Direction
- restorede0
- Qualifier
- mM and micro M concentrations of K+e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of OsHKT1 in the yeast strain CY162 defective in K+-uptake restored growth at mM and micro M concentrations of K+”
t0“rice”
Claim · model-written summary
OsHKT1 expression mediated yeast hypersensitivity to Na+.
- Taxon
- riceOryza sativat0
- Outcome
- hypersensitivity to Na+e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mediated hypersensitivity to Na+”
t0“rice”
Claim · model-written summary
OsHKT1 transported Na+ and other alkali cations in oocytes.
- Taxon
- riceOryza sativae0
- Gene
- OsHKT1e0
- Outcome
- transport of other alkali cationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice OsHKT1 showed uptake characteristics of a Na+-transporter but mediated transport of other alkali cations as well”
Claim · model-written summary
OsHKT1 was expressed in roots and leaves.
- Taxon
- riceOryza sativat0
- Gene
- OsHKT1e0
- Outcome
- expressed in roots and leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHKT1 is expressed in roots and leaves”
t0“rice”
Claim · model-written summary
Na+, Rb+, Li+, and Cs+ exposure reduced OsHKT1 transcripts in both varieties.
- Taxon
- riceOryza sativat0
- Treatment
- Na+, Rb+, Li+, and Cs+e0
- Gene
- OsHKT1e0
- Outcome
- OsHKT1 transcript amountse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exposure to Na+, Rb+, Li+, and Cs+ reduced OsHKT1 transcript amounts in both varieties”
t0“rice”
Claim · model-written summary
Some conditions yielded incompletely spliced transcripts.
- Taxon
- riceOryza sativat0
- Outcome
- incompletely spliced transcriptse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in some cases, incompletely spliced transcripts were observed”
t0“rice”
Claim · model-written summary
OsHKT1 expression localized to root epidermis and vascular tissue.
- Taxon
- riceOryza sativat0
- Gene
- OsHKT1e0
- Outcome
- localized to the root epidermis and the vascular tissue inside the endodermise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of OsHKT1 was localized to the root epidermis and the vascular tissue inside the endodermis”
t0“rice”
Claim · model-written summary
Leaf OsHKT1 signal was strongest around vasculature.
- Taxon
- riceOryza sativat0
- Gene
- OsHKT1e0
- Outcome
- strongest signals in cells surrounding the vasculaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, OsHKT1 showed strongest signals in cells surrounding the vasculature”
t0“rice”
Claim · model-written summary
Salt repression of OsHKT1 differed across cell types between varieties, especially root vascular tissue.
- Taxon
- riceOryza sativae0
- Organ / stage
- root vascular tissuee0
- Gene
- OsHKT1e0
- Outcome
- repression of OsHKT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The repression of OsHKT1 in the two rice varieties during salt stress was different in various cell types with main differences in the root vascular tissue”
The same questions in other papers
transgenic · growth · Oryza sativa (rice)Positive and negative results
30 papers in the same direction as this paper · 13 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- negative
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