Paper · 2002 · Journal of Food Biochemistry

INVOLVEMENT OF PEROXIDASE AND POLYPHENOL OXIDASE IN MANGO SAP-INJURY

K. SABY JOHN; S.G. BHAT; U.J.S. PRASADA RAO

10
claims
18
verbatim quotes
17
citations
3
research areas
—
Not open access

Abstract

Sap (latex) that oozes out from mango during harvest, upon contact with the fruit, causes dark spots (sap-injury) on the peel and reduces consumer acceptance and shelf-life of fruit. In this investigation different components responsible for sap-injury were identified. Mango saps from four Indian varieties were collected and separated into aqueous and nonaqueous phases. Whole sap, aqueous phase and nonaqueous phase were tested for their ability to cause sap-injury (browning) on mangoes. The nonaqueous phase caused maximum injury and the extent of injury caused by nonaqueous phases from different varieties was varied. Limonene, ocimene and β-myrcene, the major terpenoids identified in saps of Indian varieties, caused injury. Similar type of injury on mangoes was also caused by organic solvents. Damage on Totapuri mango fruit was significantly lower compared to other varieties, whereas Totapuri nonaqueous phase caused injury on all other varieties. The peel of Totapuri variety had very low level of polyphenol oxidase, peroxidase and polyphenols compared to other varieties. Thus, a clear relation was found between the peel polyphenol oxidase, peroxidase activities, the polyphenol content in the peel and the extent of injury. Further, nonaqueous phase applied on peels previously heat-treated at 95C for 5 min, neither caused injury nor showed any enzyme activity. Thus, the results indicated that the terpenoid components of sap and polyphenol oxidase, peroxidase, polyphenols of peel are involved in sap-injury.

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Claims 10

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The nonaqueous sap phase caused the greatest mango injury.

Treatment
nonaqueous phasee0
Outcome
injurye0
Direction
maximume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The nonaqueous phase caused maximum injury”

✓ located verbatim in the source · abstract, characters 492–534 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Injury from nonaqueous phases varied among varieties.

Treatment
nonaqueous phasese0
Outcome
extent of injurye0
Qualifier
different varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the extent of injury caused by nonaqueous phases from different varieties was varied”

✓ located verbatim in the source · abstract, characters 539–623 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Limonene, ocimene and β-myrcene caused mango injury.

Taxon
mangoesMangifera indicae1
Treatment
Limonene, ocimene and β-myrcenee0
Outcome
injurye0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Limonene, ocimene and β-myrcene, the major terpenoids identified in saps of Indian varieties, caused injury”

✓ located verbatim in the source · abstract, characters 625–732 · show in text ↑
e1

“browning) on mangoes”

✓ located verbatim in the source · abstract, characters 470–490 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Organic solvents also caused mango injury.

Taxon
mangoesMangifera indicae0
Treatment
organic solventse0
Outcome
injurye0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Similar type of injury on mangoes was also caused by organic solvents”

✓ located verbatim in the source · abstract, characters 734–803 · show in text ↑
Observationobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Totapuri mango fruit had significantly less damage than other varieties.

Material
Totapurie0
Outcome
Damagee0
Direction
lowere0
Comparator
other varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Damage on Totapuri mango fruit was significantly lower compared to other varieties”

✓ located verbatim in the source · abstract, characters 805–887 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Totapuri nonaqueous phase injured all other varieties.

Treatment
Totapuri nonaqueous phasee0
Outcome
injurye0
Qualifier
all other varietiese0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Totapuri nonaqueous phase caused injury on all other varieties”

✓ located verbatim in the source · abstract, characters 897–959 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Totapuri peel had very low polyphenol oxidase, peroxidase and polyphenols relative to other varieties.

Material
Totapuri varietye0
Organ / stage
peele0
Outcome
polyphenol oxidase, peroxidase and polyphenolse0
Direction
very lowe0
Comparator
other varietiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The peel of Totapuri variety had very low level of polyphenol oxidase, peroxidase and polyphenols compared to other varieties”

✓ located verbatim in the source · abstract, characters 961–1086 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Peel enzyme activities and polyphenol content were related to injury extent.

Organ / stage
peele0
Outcome
extent of injurye0
Qualifier
polyphenol oxidase, peroxidase activities, the polyphenol contente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a clear relation was found between the peel polyphenol oxidase, peroxidase activities, the polyphenol content in the peel and the extent of injury”

✓ located verbatim in the source · abstract, characters 1094–1240 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Nonaqueous phase caused no injury after peel heat treatment at 95C for 5 min.

Treatment
heat-treated at 95C for 5 mine0
Outcome
injurye0
Direction
neither causede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“nonaqueous phase applied on peels previously heat-treated at 95C for 5 min, neither caused injury nor showed any enzyme activity”

✓ located verbatim in the source · abstract, characters 1251–1379 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Heat-treated peels showed no enzyme activity after nonaqueous phase application.

Treatment
heat-treated at 95C for 5 mine0
Outcome
enzyme activitye0
Direction
nor showed anye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Mangifera indica
Mangifera indica · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“nonaqueous phase applied on peels previously heat-treated at 95C for 5 min, neither caused injury nor showed any enzyme activity”

✓ located verbatim in the source · abstract, characters 1251–1379 · show in text ↑
t0

“mango”

✓ located after whitespace/quote normalisation · title, characters 52–57 · show in text ↑

limonene · crop injury · Mangifera indica (mango)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

heat · crop injury · Mangifera indica (mango)Results differ

15 papers: 12 positive, 3 negative or no effect

11 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. no effect

11 papers more in this group

See all 15 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.