Abstract
Stevia rebaudiana Bertoni is a unique medicinal plant which is mostly utilized as a sugar substitute for diabetic patients. In this research, regenerated plantlets of stevia in tissue culture is transferred to pots in greenhouse and inoculated with plant-growth-promoting rhizobacteria (PGPRs) (Bacillus polymixa, Pseudomonas putida, and Azotobacter chroococcum) and arbuscular mycorrhizal fungus (AMF) (Glomus intraradices). The results showed that in comparison to control, inoculation with a single microorganism, significantly increased root and shoot biomass as well as stevioside, chlorophyll, and NPK content in plants. However, such increased effects have been found to be further enhanced significantly due to dual compatible mixtures of inoculants resulting from their strong synergistic relationships among themselves. All growth parameters recorded the highest in 60-days-old plants in the treatment of Glomus+Azotobacter and followed with Glomus+Bacillus and Azotobacter+Pseudomonas treatments, respectively. Furthermore, high-performance liquid chromatography (HPLC) chromatograms revealed that the highest stevioside content have been produced in same treatments. Triple treatments had less positive effects compared to dual inoculations. Probably competence between microorganisms in triple inoculations has reduced their efficiency. Thus, suitable combination of mycorrhizal fungi and PGPR as biotic elicitors can enhance growth and stevioside content in tissue culture-regenerated plantlets of stevia.
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Claims 12
Claim · model-written summary
Single-microorganism inoculation significantly increased root and shoot biomass relative to control.
- Taxon
- Stevia rebaudiana
Stevia rebaudianat0 - Treatment
- inoculation with a single microorganisme0
- Outcome
- root and shoot biomasse0
- Direction
- increasede0
- Comparator
- controle0
Not reportedSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that in comparison to control, inoculation with a single microorganism, significantly increased root and shoot biomass as well as stevioside, chlorophyll, and NPK content in plants.”
t0“Stevia rebaudiana”
Claim · model-written summary
Single-microorganism inoculation significantly increased stevioside relative to control.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Treatment
- inoculation with a single microorganisme0
- Outcome
- steviosidee0
- Direction
- increasede0
- Comparator
- controle0
Not reportedSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that in comparison to control, inoculation with a single microorganism, significantly increased root and shoot biomass as well as stevioside, chlorophyll, and NPK content in plants.”
t0“Stevia rebaudiana”
Claim · model-written summary
Single-microorganism inoculation significantly increased chlorophyll relative to control.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Treatment
- inoculation with a single microorganisme0
- Outcome
- chlorophylle0
- Direction
- increasede0
- Comparator
- controle0
Not reportedSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that in comparison to control, inoculation with a single microorganism, significantly increased root and shoot biomass as well as stevioside, chlorophyll, and NPK content in plants.”
t0“Stevia rebaudiana”
Claim · model-written summary
Single-microorganism inoculation significantly increased NPK content relative to control.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Treatment
- inoculation with a single microorganisme0
- Outcome
- NPK contente0
- Direction
- increasede0
- Comparator
- controle0
Not reportedSetting
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that in comparison to control, inoculation with a single microorganism, significantly increased root and shoot biomass as well as stevioside, chlorophyll, and NPK content in plants.”
t0“Stevia rebaudiana”
Claim · model-written summary
Dual compatible inoculant mixtures significantly enhanced the positive growth and content effects.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Treatment
- dual compatible mixtures of inoculantse0
- Outcome
- such increased effectse0
- Direction
- further enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, such increased effects have been found to be further enhanced significantly due to dual compatible mixtures of inoculants resulting from their strong synergistic relationships among themselves.”
t0“Stevia rebaudiana”
Claim · model-written summary
Glomus+Azotobacter ranked among the leading inoculant combinations for growth parameters in 60-day-old stevia.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Organ / stage
- 60-days-old plantse0
- Treatment
- Glomus+Azotobactere0
- Outcome
- growth parameterse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All growth parameters recorded the highest in 60-days-old plants in the treatment of Glomus+Azotobacter and followed with Glomus+Bacillus and Azotobacter+Pseudomonas treatments, respectively.”
t0“Stevia rebaudiana”
Claim · model-written summary
Glomus+Bacillus ranked among the leading inoculant combinations for growth parameters in 60-day-old stevia.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Organ / stage
- 60-days-old plantse0
- Treatment
- Glomus+Bacilluse0
- Outcome
- growth parameterse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All growth parameters recorded the highest in 60-days-old plants in the treatment of Glomus+Azotobacter and followed with Glomus+Bacillus and Azotobacter+Pseudomonas treatments, respectively.”
t0“Stevia rebaudiana”
Claim · model-written summary
Azotobacter+Pseudomonas ranked among the leading inoculant combinations for growth parameters in 60-day-old stevia.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Organ / stage
- 60-days-old plantse0
- Treatment
- Azotobacter+Pseudomonase0
- Outcome
- growth parameterse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All growth parameters recorded the highest in 60-days-old plants in the treatment of Glomus+Azotobacter and followed with Glomus+Bacillus and Azotobacter+Pseudomonas treatments, respectively.”
t0“Stevia rebaudiana”
Claim · model-written summary
The leading inoculant combinations produced the highest stevioside content.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Outcome
- stevioside contente0
- Direction
- higheste0
- Qualifier
- same treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, high-performance liquid chromatography (HPLC) chromatograms revealed that the highest stevioside content have been produced in same treatments.”
t0“Stevia rebaudiana”
Claim · model-written summary
Triple inoculations had less positive effects than dual inoculations.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Treatment
- Triple treatmentse0
- Outcome
- positive effectse0
- Direction
- lesse0
- Comparator
- dual inoculationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Triple treatments had less positive effects compared to dual inoculations.”
t0“Stevia rebaudiana”
Claim · model-written summary
Competition between microorganisms may have reduced the efficiency of triple inoculations.
- Taxon
- Stevia rebaudianaStevia rebaudianat0
- Outcome
- efficiencye0
- Qualifier
- competence between microorganisms in triple inoculationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Probably competence between microorganisms in triple inoculations has reduced their efficiency.”
t0“Stevia rebaudiana”
Claim · model-written summary
The authors recommend suitable mycorrhizal fungi and PGPR combinations to enhance regenerated stevia growth and stevioside content.
SteviaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thus, suitable combination of mycorrhizal fungi and PGPR as biotic elicitors can enhance growth and stevioside content in tissue culture-regenerated plantlets of stevia.”
The same questions in other papers
inoculation · growth · Stevia rebaudianaPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →inoculation · nutrient: content · Stevia rebaudianaResults agree
1 paper in the same direction as this paper
- positive
inoculation · physiology: pigments · Stevia rebaudianaResults agree
1 paper in the same direction as this paper
- positive
inoculation · molecular: metabolites · Stevia rebaudianaResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.