Abstract
BACKGROUND AND AIMS: The metabolism of beta-1,3 : 1,4-glucan regulates the mechanical properties of cell walls, and thereby changes the elongation growth of Poaceae plants. A previous study has shown that elongation growth of rice coleoptiles under water is enhanced by increased activity of beta-1,3 : 1,4-glucan hydrolases; however, the involvement of beta-1,3 : 1,4-glucan synthase activity in elongation growth under water has not yet been clarified. METHODS: The beta-1,3 : 1,4-glucan synthase activity in a microsomal fraction prepared from rice seedlings grown under water was compared with that from control seedlings grown in air. The change under water in the relative expression level of CslF6, a major isoform of the beta-1,3 : 1,4-glucan synthase genes, was examined by quantitative reverse-transcriptase PCR. KEY RESULTS: The level of beta-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40 % of that of the control seedlings and was accompanied by a significant reduction in the amount of beta-1,3 : 1,4-glucan in the cell walls. Under water, the expression of CslF6 was reduced to less than 20 % of the unsubmerged control. Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water, correlating with suppression of the submergence-induced elongation growth of coleoptiles. CONCLUSIONS: Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of beta-1,3 : 1,4-glucan synthase activity. Together with the increased activity of beta-1,3 : 1,4-glucan hydrolases, the decreased activity of beta-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of beta-1,3 : 1,4-glucan in the cell walls under water. The suppression of beta-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion.
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Claims 9
Claim · model-written summary
Submergence reduced beta-1,3 : 1,4-glucan synthase activity to less than 40% of air-grown controls.
- Taxon
- Rice
Oryza sativat0 - Treatment
- submergede0
- Outcome
- beta-1,3 : 1,4-glucan synthase activitye0
- Direction
- decreasede0
- Value
- less than 40 %e0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of beta-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40 % of that of the control seedlings”
t0“Rice Seedlings”
Claim · model-written summary
Submergence significantly reduced cell-wall beta-1,3 : 1,4-glucan amount.
- Taxon
- RiceOryza sativat0
- Treatment
- under watert0
- Outcome
- amount of beta-1,3 : 1,4-glucan in the cell wallse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant reduction in the amount of beta-1,3 : 1,4-glucan in the cell walls”
t0“Rice Seedlings under Water”
Claim · model-written summary
Under water, CslF6 expression fell below 20% of the unsubmerged control.
- Taxon
- RiceOryza sativat0
- Treatment
- Under watere0
- Gene
- CslF6e0
- Outcome
- expression of CslF6e0
- Direction
- reducede0
- Value
- less than 20 %e0
- Comparator
- unsubmerged controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under water, the expression of CslF6 was reduced to less than 20 % of the unsubmerged control”
t0“Rice Seedlings”
Claim · model-written summary
Bubble aeration partially restored glucan synthase activity under water.
- Taxon
- RiceOryza sativat0
- Treatment
- Bubble aeratione0
- Outcome
- beta-1,3 : 1,4-glucan synthase activitye0
- Direction
- partially restorede0
- Qualifier
- under watere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water”
t0“Rice Seedlings”
Claim · model-written summary
Bubble aeration partially restored CslF6 expression under water.
- Taxon
- RiceOryza sativat0
- Treatment
- Bubble aeratione0
- Gene
- CslF6e0
- Outcome
- expression of CslF6e0
- Direction
- partially restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water”
t0“Rice Seedlings”
Claim · model-written summary
Aeration-related recovery of synthesis and CslF6 expression correlated with suppression of submergence-induced coleoptile elongation.
- Taxon
- RiceOryza sativat0
- Organ / stage
- coleoptilese0
- Outcome
- submergence-induced elongation growth of coleoptilese0
- Direction
- suppressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“correlating with suppression of the submergence-induced elongation growth of coleoptiles”
t0“Rice Seedlings”
Claim · model-written summary
The authors attribute the submergence decline in glucan synthase activity to downregulation of CslF6.
- Taxon
- RiceOryza sativat0
- Treatment
- Submergencee0
- Gene
- CslF6e0
- Outcome
- decreased level of beta-1,3 : 1,4-glucan synthase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of beta-1,3 : 1,4-glucan synthase activity”
t0“Rice Seedlings”
Claim · model-written summary
The authors attribute reduced cell-wall glucan to lower synthase activity together with increased hydrolase activity.
- Taxon
- RiceOryza sativat0
- Gene
- beta-1,3 : 1,4-glucan hydrolasese0
- Outcome
- decrease in the amount of beta-1,3 : 1,4-glucan in the cell wallse0
- Qualifier
- decreased activity of beta-1,3 : 1,4-glucan synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Together with the increased activity of beta-1,3 : 1,4-glucan hydrolases, the decreased activity of beta-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of beta-1,3 : 1,4-glucan in the cell walls under water”
t0“Rice Seedlings”
Claim · model-written summary
The authors suggest oxygen depletion mainly explains suppressed glucan synthesis under water.
- Taxon
- RiceOryza sativat0
- Outcome
- suppression of beta-1,3 : 1,4-glucan synthesise0
- Qualifier
- oxygen depletione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suppression of beta-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion”
t0“Rice Seedlings”
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flooding · physiology: enzyme activity · Oryza sativa (rice)Positive and negative results
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.