Abstract
Hypocotyls of Fenugreek (Trigonella foenum-graecum L.) were cut into 5 mm long segments and used as explants. For obtaining callus under various water and salt stress levels, MS supplemented with 2.0 mg/l BA and 1.0 mg/l NAA was prepared; then divided into nine portions, the first of which was kept as control while to the other portions mannitol (0.1 and 0.3% w/v) and sodium chloride (0.1 and 0.3% w/v) were added separately and interacted. Callus formation was allowed to take place for a period of six weeks in the dark at 250C. Calli from various treatments were then harvested and subjected to growth, total phenolics, total flavonoids and total tannin contents determinations. Results of the present study have shown that the lower concentration of mannitol enhanced growth while the higher concentration enhanced growth, total phenolics and total flavonoid contents in comparison to the control. Mild salt stress which was created by the lower sodium chloride concentration reduced growth with concomitant increase in total phenolics, total flavonoids and total tannins while the higher salt concentration significantly decreased growth and secondary metabolite accumulation as well. Interaction treatments between mannitol and sodium chloride exhibited various effects on calli growth and secondary metabolites levels. This study throws some light on the behavior of cultured fenugreek cells grown under water and salt stress. Key words: Trigonella foenum-gaecum, callus, abiotic stress, secondary metabolites D.O.I. 10.3329/ptcb.v21i1.9561 Plant Tissue Cult. & Biotech. 21(1): 35-43, 2011 (June)
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Claims 7
Claim · model-written summary
Lower mannitol concentration enhanced fenugreek callus growth.
- Taxon
- Fenugreek
Trigonella foenum-graecumt0 - Treatment
- lower concentration of mannitole0
- Outcome
- growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lower concentration of mannitol enhanced growth”
t0“Fenugreek”
Claim · model-written summary
Higher mannitol concentration enhanced growth, total phenolics and total flavonoids versus control.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- higher concentratione0
- Outcome
- growth, total phenolics and total flavonoid contentse0
- Direction
- enhancede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher concentration enhanced growth, total phenolics and total flavonoid contents in comparison to the control”
t0“Fenugreek”
Claim · model-written summary
Lower sodium chloride concentration reduced callus growth.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- lower sodium chloride concentratione0
- Outcome
- growthe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lower sodium chloride concentration reduced growth”
t0“Fenugreek”
Claim · model-written summary
Lower sodium chloride concentration increased total phenolics, flavonoids and tannins.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- lower sodium chloride concentratione0
- Outcome
- total phenolics, total flavonoids and total tanninse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lower sodium chloride concentration reduced growth with concomitant increase in total phenolics, total flavonoids and total tannins”
t0“Fenugreek”
Claim · model-written summary
Higher salt concentration significantly decreased callus growth.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- higher salt concentratione0
- Outcome
- growthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher salt concentration significantly decreased growth and secondary metabolite accumulation as well”
t0“Fenugreek”
Claim · model-written summary
Higher salt concentration significantly decreased secondary metabolite accumulation.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- higher salt concentratione0
- Outcome
- secondary metabolite accumulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the higher salt concentration significantly decreased growth and secondary metabolite accumulation as well”
t0“Fenugreek”
Claim · model-written summary
Combined mannitol and sodium chloride treatments had varying effects on callus growth and secondary metabolites.
- Taxon
- FenugreekTrigonella foenum-graecumt0
- Treatment
- Interaction treatments between mannitol and sodium chloridee0
- Outcome
- calli growth and secondary metabolites levelse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction treatments between mannitol and sodium chloride exhibited various effects on calli growth and secondary metabolites levels”
t0“Fenugreek”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.