Abstract
GAMYB is a component of gibberellin (GA) signaling in cereal aleurone cells, and has an important role in flower development. However, it is unclear how GAMYB function is regulated. We examined the involvement of a microRNA, miR159, in the regulation of GAMYB expression in cereal aleurone cells and flower development. In aleurone cells, no miR159 expression was observed with or without GA treatment, suggesting that miR159 is not involved in the regulation of GAMYB and GAMYB-like genes in this tissue. miR159 was expressed in tissues other than aleurone, and miR159 over-expressors showed similar but more severe phenotypes than the gamyb mutant. GAMYB and GAMYB-like genes are co-expressed with miR159 in anthers, and the mRNA levels for GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during anther development. Thus, OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers. A microarray analysis revealed that OsGAMYB and its upstream regulator SLR1 are involved in the regulation of almost all GA-mediated gene expression in rice aleurone cells. Moreover, different sets of genes are regulated by GAMYB in aleurone cells and anthers. GAMYB binds directly to promoter regions of its target genes in anthers as well as aleurone cells. Based on these observations, we suggest that the regulation of GAMYB expression and GAMYB function are different in aleurone cells and flowers in rice.
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Claims 9
Claim · model-written summary
No miR159 expression was observed in rice aleurone cells with or without GA treatment
- Taxon
- rice
Oryza sativae2 - Organ / stage
- aleurone cellse2
- Treatment
- GA treatmente1
- Gene
- miR159e1
- Outcome
- no miR159 expressione1
- Comparator
- without GA treatmente1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“no miR159 expression was observed with or without GA treatment”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
miR159 is expressed in rice tissues other than aleurone
- Taxon
- riceOryza sativae2
- Organ / stage
- tissues other than aleuronee1
- Gene
- miR159e1
- Outcome
- expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“miR159 was expressed in tissues other than aleurone”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
miR159 over-expressors show similar but more severe phenotypes than the gamyb mutant in rice
- Taxon
- riceOryza sativae2
- Treatment
- miR159 over-expressorse1
- Outcome
- phenotypese1
- Direction
- more severee1
- Comparator
- gamyb mutante1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“miR159 over-expressors showed similar but more severe phenotypes than the gamyb mutant”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
mRNA levels of GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during rice anther development
- Taxon
- riceOryza sativae2
- Organ / stage
- anther developmente1
- Gene
- GAMYB and GAMYB-like genese1
- Outcome
- mRNA levelse1
- Direction
- negatively correlatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the mRNA levels for GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during anther development”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
GAMYB and GAMYB-like genes are co-expressed with miR159 in rice anthers
- Taxon
- riceOryza sativae2
- Organ / stage
- antherse1
- Gene
- GAMYB and GAMYB-like genese1
- Outcome
- co-expressede1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GAMYB and GAMYB-like genes are co-expressed with miR159 in anthers”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
OsGAMYB and OsGAMYB-like genes are regulated by miR159 in rice flowers
- Taxon
- riceOryza sativae2
- Organ / stage
- flowerse1
- Gene
- OsGAMYB and OsGAMYB-like genese1
- Outcome
- regulated by miR159e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
OsGAMYB and its upstream regulator SLR1 are involved in regulating almost all GA-mediated gene expression in rice aleurone cells
- Taxon
- riceOryza sativae1
- Organ / stage
- aleurone cellse1
- Gene
- OsGAMYB and its upstream regulator SLR1e1
- Outcome
- regulation of almost all GA-mediated gene expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“OsGAMYB and its upstream regulator SLR1 are involved in the regulation of almost all GA-mediated gene expression in rice aleurone cells”
Claim · model-written summary
Different sets of genes are regulated by GAMYB in rice aleurone cells and anthers
- Taxon
- riceOryza sativae2
- Organ / stage
- aleurone cells and antherse1
- Gene
- GAMYBe1
- Outcome
- different sets of genese1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“different sets of genes are regulated by GAMYB in aleurone cells and anthers”
e2“aleurone cells and flowers in rice”
Claim · model-written summary
GAMYB binds directly to promoter regions of its target genes in rice anthers and aleurone cells
- Taxon
- riceOryza sativae2
- Organ / stage
- antherse1
- Gene
- GAMYBe1
- Outcome
- binds directly to promoter regionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“GAMYB binds directly to promoter regions of its target genes in anthers as well as aleurone cells”
e2“aleurone cells and flowers in rice”
The same questions in other papers
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