Abstract
This study investigated the allelopathic, genotoxic and cytotoxic effects of the tuber extracts of Dioscorea dumetorum. This was with a view to eventually identifying herbicides and anti-tumor compounds from the plant. Methanol extract and hexane fraction of pulverized D. dumetorum was obtained. Tests for allelopathy, genotoxicity and cytotoxicity were carried out, along with proline content, total protein and total sugar assays. The hexane fraction exhibited a potent growth inhibitory effect. The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction. The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml. The extract and fraction also inhibited seed germination in a dose-dependent manner. Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation and multiple chromatin breaks. The proline content of the onions treated with the extracts increased compared to the control group. There was a significant decrease in protein content in the onions treated with the extracts compared to the control group. Total soluble sugar content increased significantly in the treatment group compared to the control group. The LC50 values obtained in a brine shrimp lethality bioassay of the extracts showed the hexane fraction to be more potent than the methanolic extract. It was 26 µg/ml and 51 µg/ml respectively, which are considerably lower than 1 mg/ml, which is usually considered active. The study showed that the plant possessed allelopathic, genotoxic and cytotoxic potentials.
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Claims 12
Claim · model-written summary
The hexane fraction inhibited growth by 42% at 15 mg/ml.
- Taxon
- Dioscorea Dumetorum
Dioscorea dumetorumt0 - Treatment
- fractione0
- Outcome
- inhibitione0
- Direction
- increasede0
- Value
- 42%e0
- Qualifier
- 15 mg/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The hexane fraction inhibited growth by 92% at 60 mg/ml.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- fractione0
- Outcome
- inhibitione0
- Direction
- increasede0
- Value
- 92%e0
- Qualifier
- 60 mg/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition increased from 42% at 15 mg/ml to 92% at 60 mg/ml of the fraction.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The methanolic extract produced 25% inhibition at 20 mg/ml and was less potent than the hexane fraction.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- methanolic extracte0
- Outcome
- inhibitione0
- Direction
- increasede0
- Value
- 25%e0
- Qualifier
- 20 mg/mle0
- Comparator
- hexane fractione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The methanolic extract was less potent than the hexane fraction, the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The methanolic extract produced 75% inhibition at 80 mg/ml.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Outcome
- inhibitione0
- Direction
- increasede0
- Value
- 75%e0
- Qualifier
- 80 mg/mle0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inhibition increased from 25% at 20 mg/ml to 75% at 80 mg/ml.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The extract inhibited seed germination in a dose-dependent manner.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- extracte0
- Outcome
- seed germinatione0
- Direction
- inhibitede0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extract and fraction also inhibited seed germination in a dose-dependent manner.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The fraction inhibited seed germination in a dose-dependent manner.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- fractione0
- Outcome
- seed germinatione0
- Direction
- inhibitede0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extract and fraction also inhibited seed germination in a dose-dependent manner.”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
Allium cepa exposed to the extracts showed multiple chromosomal abnormalities.
- Taxon
- Allium cepa
Allium cepae0 - Outcome
- chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation and multiple chromatin breakse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genotoxicity studies using Allium cepa showed different chromosomal aberrations like fragmentation, cell with bloated nucleus, fuzzy chromosome, puffy chromosome, chromatin erosion, late condensation and multiple chromatin breaks.”
Claim · model-written summary
Onions treated with the extracts had increased proline content relative to controls.
- Taxon
- onionsAllium cepae0
- Treatment
- extractse0
- Outcome
- proline contente0
- Direction
- increasede0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proline content of the onions treated with the extracts increased compared to the control group.”
Claim · model-written summary
Onions treated with the extracts had significantly decreased protein content relative to controls.
- Taxon
- onionsAllium cepae0
- Treatment
- extractse0
- Outcome
- protein contente0
- Direction
- decreasee0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was a significant decrease in protein content in the onions treated with the extracts compared to the control group.”
Claim · model-written summary
Total soluble sugar content increased significantly in the treatment group.
- Taxon
- onionsAllium cepae1
- Treatment
- extractse1
- Outcome
- Total soluble sugar contente0
- Direction
- increasede0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total soluble sugar content increased significantly in the treatment group compared to the control group.”
e1“protein content in the onions treated with the extracts”
Claim · model-written summary
The hexane fraction had an LC50 of 26 µg/ml in the brine shrimp assay.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- hexane fractione0
- Outcome
- LC50 valuese0
- Direction
- more potente0
- Value
- 26 µg/mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LC50 values obtained in a brine shrimp lethality bioassay of the extracts showed the hexane fraction to be more potent than the methanolic extract. It was 26 µg/ml and 51 µg/ml respectively”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
Claim · model-written summary
The methanolic extract had an LC50 of 51 µg/ml in the brine shrimp assay.
- Taxon
- Dioscorea DumetorumDioscorea dumetorumt0
- Treatment
- methanolic extracte0
- Outcome
- LC50 valuese0
- Value
- 51 µg/mle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The LC50 values obtained in a brine shrimp lethality bioassay of the extracts showed the hexane fraction to be more potent than the methanolic extract. It was 26 µg/ml and 51 µg/ml respectively”
t0“Dioscorea Dumetorum Kunth Tuber extracts”
The same questions in other papers
botanical · quality: protein · Allium cepa (onion)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.