Abstract
Leaf protoplast-derived cells of the embryogenic alfalfa genotype (Medicago sativa ssp. varia A2) follow different fate if cultured in the presence of 0.25, 1 or 10 µM 2,4- dichlorophenoxyacetic acid (2,4-D). Cells grown in the presence of the highest auxin (2,4-D) concentration become embryogenic and can develop into somatic embryos if subcultured into fresh medium with the lower 2,4-D level. Cells cultured at the lower auxin concentrations from the beginning develop into elongated differentiated cells. In order to reveal physiological changes that characterize the reactivation of cell division in resting cells as well as the transition of somatic plant cells to an embryogenic state, morphological, cell division, intracellular pH and stress-related parameters have been determined during the first five days of parallel cultures at the above 2,4-D concentrations in combination with stress treatments.
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Claims 2
Claim · model-written summary
Cells at the highest 2,4-D concentration became embryogenic and could form somatic embryos after transfer to lower 2,4-D.
- Taxon
- alfalfa
Medicago sativat0 - Material
- Medicago sativa ssp. varia A2e0
- Treatment
- highest auxin (2,4-D) concentratione0
- Outcome
- become embryogenic and can develop into somatic embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf protoplast-derived cells of the embryogenic alfalfa genotype (Medicago sativa ssp. varia A2) follow different fate if cultured in the presence of 0.25, 1 or 10 µM 2,4- dichlorophenoxyacetic acid (2,4-D). Cells grown in the presence of the highest auxin (2,4-D) concentration become embryogenic and can develop into somatic embryos if subcultured into fresh medium with the lower 2,4-D level”
t0“alfalfa”
Claim · model-written summary
Cells cultured from the outset at lower auxin concentrations developed into elongated differentiated cells.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- lower auxin concentrationse0
- Outcome
- elongated differentiated cellse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells cultured at the lower auxin concentrations from the beginning develop into elongated differentiated cells.”
t0“alfalfa”
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