Abstract
In vitro shoots of vegetative rootstocks for cherry (Gisela 5 and Gisela 6), plum (Fereley Jaspi) and pear (Pyrodwarf) were repeatedly subcultured for 10 subcultures on Murashige and Skoog medium of unchanged hormonal composition. Shoot formation capacity decreased over repeated subculturing in all genotypes. The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi, while in Gisela 5 the decline occurred after second subculture, and remained at that level. As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed. Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined. This irreversible decline could be due to residual effects of hormones. However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype. Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards). After subculturing, several media were evaluated for induction of rhizogenesis in order to achieve high rooting rates in tested rootstocks. The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%). Rooted shoots were successfully acclimatized under the mist system in greenhouse.
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Claims 20
Claim · model-written summary
Gisela 6 multiplication first significantly decreased after the first subculture, was mainly stable from second to fourth, then declined again.
- Taxon
- cherrye2
- Material
- Gisela 6e0
- Treatment
- first subculturee0
- Outcome
- multiplication indexe0
- Direction
- decreasee0
- Qualifier
- mainly stable from second to forth subculturee1
Not reportedComparator
Verbatim
e0“The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi”
e1“As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed.”
e2“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Fereley Jaspi multiplication first significantly decreased after the first subculture, was mainly stable from second to fourth, then declined again.
- Taxon
- plum
Prunus domesticae2 - Material
- Fereley Jaspie0
- Treatment
- first subculturee0
- Outcome
- multiplication indexe0
- Direction
- decreasee0
- Qualifier
- mainly stable from second to forth subculturee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The first significant decrease in multiplication index was observed after first subculture in Gisela 6 and Fereley Jaspi”
e1“As for Gisela 6 and Fereley Jaspi, multiplication index was mainly stable from second to forth subculture, whereupon the second decline in shoot formation was observed.”
e2“plum (Fereley Jaspi)”
Claim · model-written summary
Gisela 5 multiplication declined after the second subculture and remained at that level.
- Taxon
- cherrye2
- Material
- Gisela 5e0
- Treatment
- second subculturee0
- Outcome
- multiplication indexe1
- Direction
- declinee0
- Qualifier
- remained at that levele0
Not reportedComparator
Verbatim
e0“while in Gisela 5 the decline occurred after second subculture, and remained at that level.”
e1“The first significant decrease in multiplication index”
e2“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Pyrodwarf had very low multiplication capacity but multiplication slightly increased over the first three subcultures.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise1 - Material
- Pyrodwarfe0
- Treatment
- first three subculturese0
- Outcome
- shoot multiplicatione0
- Direction
- increasede0
- Qualifier
- very low multiplication capacitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined.”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Pyrodwarf shoot multiplication declined after the first three subcultures.
- Taxon
- pearPyrus communise1
- Material
- Pyrodwarfe0
- Treatment
- subculturese0
- Outcome
- shoot multiplicatione0
- Direction
- declinede0
- Qualifier
- first three subculturese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although Pyrodwarf showed very low multiplication capacity, shoot multiplication slightly increased over the first three subcultures and then declined.”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Residual hormone effects may explain irreversible multiplication decline in cherry rootstocks.
Verbatim
e0“This irreversible decline could be due to residual effects of hormones.”
e1“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Residual hormone effects may explain irreversible multiplication decline in plum rootstock.
- Taxon
- plumPrunus domesticae1
- Compound
- hormonese0
- Outcome
- irreversible declinee0
- Qualifier
- could be due to residual effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This irreversible decline could be due to residual effects of hormones.”
e1“plum (Fereley Jaspi)”
Claim · model-written summary
Residual hormone effects may explain irreversible multiplication decline in pear rootstock.
- Taxon
- pearPyrus communise1
- Compound
- hormonese0
- Outcome
- irreversible declinee0
- Qualifier
- could be due to residual effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This irreversible decline could be due to residual effects of hormones.”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Cherry shoots showed no visible morphological variations or aberrations during successive subcultures.
Verbatim
e0“However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype.”
e1“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Plum shoots showed no visible morphological variations or aberrations during successive subcultures.
- Taxon
- plumPrunus domesticae1
- Material
- Fereley Jaspie1
- Outcome
- no visible morphological variations or aberrations of shootse0
- Qualifier
- successive subculturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype.”
e1“plum (Fereley Jaspi)”
Claim · model-written summary
Pear shoots showed no visible morphological variations or aberrations during successive subcultures.
- Taxon
- pearPyrus communise1
- Material
- Pyrodwarfe1
- Outcome
- no visible morphological variations or aberrations of shootse0
- Qualifier
- successive subculturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, no visible morphological variations or aberrations of shoots were found in successive subcultures in any genotype.”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Cherry shoot length quality varied with subculturing and was highest from the fifth subculture onwards.
- Taxon
- cherrye1
- Treatment
- later subculturese0
- Outcome
- Quality of shoots in terms of shoot lengthe0
- Direction
- higheste0
- Qualifier
- from fifth subculture onwardse0
Not reportedComparator
Verbatim
e0“Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards).”
e1“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Plum shoot length quality varied with subculturing and was highest from the fifth subculture onwards.
- Taxon
- plumPrunus domesticae1
- Material
- Fereley Jaspie1
- Treatment
- later subculturese0
- Outcome
- Quality of shoots in terms of shoot lengthe0
- Direction
- higheste0
- Qualifier
- from fifth subculture onwardse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards).”
e1“plum (Fereley Jaspi)”
Claim · model-written summary
Pear shoot length quality varied with subculturing and was highest from the fifth subculture onwards.
- Taxon
- pearPyrus communise1
- Material
- Pyrodwarfe1
- Treatment
- later subculturese0
- Outcome
- Quality of shoots in terms of shoot lengthe0
- Direction
- higheste0
- Qualifier
- from fifth subculture onwardse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quality of shoots in terms of shoot length varied during subculturing, but the highest quality was observed in later subcultures (from fifth subculture onwards).”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Fereley Jaspi had the highest rooting ability at 100%.
- Taxon
- plumPrunus domesticae1
- Material
- Fereley Jaspie0
- Outcome
- rooting abilitye0
- Direction
- higheste0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi”
e1“plum (Fereley Jaspi)”
Claim · model-written summary
Pyrodwarf's best rooting percentage was 90%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both)”
e1“pear (Pyrodwarf)”
Claim · model-written summary
Gisela 6's best rooting percentage was 90%.
Verbatim
e0“followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both)”
e1“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Gisela 5 rooting reached 70%; rooted cherry shoots were successfully acclimatized under greenhouse mist.
- Taxon
- cherrye2
- Material
- Gisela 5e0
- Outcome
- rooting abilitye0
- Value
- 70%e0
- Qualifier
- successfully acclimatized under the mist systeme1
- Setting
- greenhousee1
Not reportedDirection
Verbatim
e0“The highest rooting ability (100%) among genotypes was observed in Fereley Jaspi, followed by Pyrodwarf and Gisela 6 (the best rooting percentage being 90% in both) and Gisela 5 (70%).”
e1“Rooted shoots were successfully acclimatized under the mist system in greenhouse.”
e2“cherry (Gisela 5 and Gisela 6)”
Claim · model-written summary
Rooted plum shoots were successfully acclimatized under greenhouse mist.
- Taxon
- plumPrunus domesticae1
- Material
- Fereley Jaspie1
- Treatment
- mist systeme0
- Outcome
- successfully acclimatizede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted shoots were successfully acclimatized under the mist system in greenhouse.”
e1“plum (Fereley Jaspi)”
Claim · model-written summary
Rooted pear shoots were successfully acclimatized under greenhouse mist.
- Taxon
- pearPyrus communise1
- Material
- Pyrodwarfe1
- Treatment
- mist systeme0
- Outcome
- successfully acclimatizede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted shoots were successfully acclimatized under the mist system in greenhouse.”
e1“pear (Pyrodwarf)”
The same questions in other papers
in vitro · pest · Prunus domestica (plum)Positive and negative results
2 in the opposite direction
- positive
- positive
in vitro · quality: general · Prunus domestica (plum)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.