Abstract
Reactive oxygen species (ROS) have been shown to have a crucial role in plant defense responses and signaling pathways. In addition, ROS also have direct toxicity against pathogens. However, the molecular mechanisms of plant ROS in the direct effects against pathogens is still unclear. To investigate the function of plant ROS in the interactions of plant and bacterial pathogens, we focused on oxyR, encoding an oxidative stress-regulated transcription factor in Pseudomonas syringae pv. tomato DC3000 (DC3000), and generated an ΔoxyR mutant. The DC3000 ΔoxyR mutant showed high sensitivity to oxidative stress in comparison with wild type and the complemented line. The host plants of DC3000, including tomato and Arabidopsis inoculated with the ΔoxyR mutant, clearly showed reduced disease symptoms as well as reduced bacterial populations. Expression profiles of DC3000 genes revealed that OxyR could regulate the expression of genes encoding ROS-detoxifying enzymes, including catalases (KatB and KatG), in response to ROS. We also demonstrated that the expression of katB could be regulated by OxyR during the infection of DC3000 in Arabidopsis. These results suggest that OxyR has an important role in the virulence of DC3000 by regulating the expression of genes related to oxidative stress.
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Claims 6
Claim · model-written summary
Tomato inoculated with the bacterial ΔoxyR mutant had reduced disease symptoms.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- inoculated with the ΔoxyR mutante0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e1pathogen
- Outcome
- disease symptomse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tomato and Arabidopsis inoculated with the ΔoxyR mutant, clearly showed reduced disease symptoms as well as reduced bacterial populations.”
e1“Pseudomonas syringae pv. tomato DC3000”
Claim · model-written summary
Arabidopsis inoculated with the bacterial ΔoxyR mutant had reduced disease symptoms.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- inoculated with the ΔoxyR mutante0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e1pathogen
- Outcome
- disease symptomse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tomato and Arabidopsis inoculated with the ΔoxyR mutant, clearly showed reduced disease symptoms as well as reduced bacterial populations.”
e1“Pseudomonas syringae pv. tomato DC3000”
Claim · model-written summary
Tomato inoculated with ΔoxyR had reduced bacterial populations.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- inoculated with the ΔoxyR mutante0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e1pathogen
- Outcome
- bacterial populationse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tomato and Arabidopsis inoculated with the ΔoxyR mutant, clearly showed reduced disease symptoms as well as reduced bacterial populations.”
e1“Pseudomonas syringae pv. tomato DC3000”
Claim · model-written summary
Arabidopsis inoculated with ΔoxyR had reduced bacterial populations.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- inoculated with the ΔoxyR mutante0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e1pathogen
- Outcome
- bacterial populationse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tomato and Arabidopsis inoculated with the ΔoxyR mutant, clearly showed reduced disease symptoms as well as reduced bacterial populations.”
e1“Pseudomonas syringae pv. tomato DC3000”
Claim · model-written summary
Bacterial OxyR regulated ROS-detoxifying enzyme genes in response to ROS.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatot0pathogen - Gene
- OxyRe0
- Outcome
- expression of genes encoding ROS-detoxifying enzymese0
- Qualifier
- in response to ROSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OxyR could regulate the expression of genes encoding ROS-detoxifying enzymes, including catalases (KatB and KatG), in response to ROS.”
t0“Pseudomonas syringae pv. tomato OxyR Is Required for Virulence in Tomato and Arabidopsis”
Claim · model-written summary
Bacterial OxyR regulated katB expression during Arabidopsis infection.
- Taxon
- ArabidopsisArabidopsise0
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatot0pathogen
- Gene
- katBe0
- Outcome
- expression of katBe0
- Qualifier
- during the infection of DC3000 in Arabidopsise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of katB could be regulated by OxyR during the infection of DC3000 in Arabidopsis.”
t0“Pseudomonas syringae pv. tomato”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato str. DC3000 · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- no effect
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1 paper more in this group
See all 5 papers →loss of function · Pseudomonas syringae pv. tomato str. DC3000 · ArabidopsisPositive and negative results
4 in the opposite direction
- no effect
- no effect
- negative
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.