Abstract
Chromatin remodelers act in an ATP-dependent manner to modulate chromatin structure and thus genome function. Here, we report that the Arabidopsis (Arabidopsis thaliana) remodeler CHROMATIN REMODELING19 (CHR19) is enriched in gene body regions, and its depletion causes massive changes in nucleosome position and occupancy in the genome. Consistent with these changes, an in vitro assay verified that CHR19 can utilize ATP to slide nucleosomes. A variety of inducible genes, including several important genes in the salicylic acid (SA) and jasmonic acid (JA) pathways, were transcriptionally upregulated in the chr19 mutant under normal growth conditions, indicative of a role of CHR19 in transcriptional repression. In addition, the chr19 mutation triggered higher susceptibility to the JA pathway-defended necrotrophic fungal pathogen Botrytis cinerea, but did not affect the growth of the SA pathway-defended hemibiotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Expression of CHR19 was tissue-specific and inhibited specifically by SA treatment. Such inhibition significantly decreased the local chromatin enrichment of CHR19 at the associated SA pathway genes, which resulted in their full activation upon SA treatment. Overall, our findings clarify CHR19 to be a novel regulator acting at the chromatin level to impact the transcription of genes underlying plant resistance to different pathogens.
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Claims 10
Claim · model-written summary
CHR19 is enriched in gene body regions.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- CHROMATIN REMODELING19 (CHR19)e0
- Outcome
- enriched in gene body regionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Arabidopsis (Arabidopsis thaliana) remodeler CHROMATIN REMODELING19 (CHR19) is enriched in gene body regions”
Claim · model-written summary
CHR19 depletion extensively changed nucleosome position and occupancy.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- its depletione0
- Gene
- CHROMATIN REMODELING19 (CHR19)e0
- Outcome
- nucleosome position and occupancye0
- Direction
- massive changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Arabidopsis (Arabidopsis thaliana) remodeler CHROMATIN REMODELING19 (CHR19) is enriched in gene body regions, and its depletion causes massive changes in nucleosome position and occupancy in the genome.”
Claim · model-written summary
CHR19 used ATP to slide nucleosomes in vitro.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CHR19e0
- Outcome
- utilize ATP to slide nucleosomese0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“an in vitro assay verified that CHR19 can utilize ATP to slide nucleosomes.”
Claim · model-written summary
Inducible genes including SA and JA pathway genes were upregulated in the chr19 mutant.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- chr19 mutante0
- Gene
- chr19e0
- Outcome
- A variety of inducible genes, including several important genes in the salicylic acid (SA) and jasmonic acid (JA) pathwayse0
- Direction
- upregulatede0
- Qualifier
- under normal growth conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“A variety of inducible genes, including several important genes in the salicylic acid (SA) and jasmonic acid (JA) pathways, were transcriptionally upregulated in the chr19 mutant under normal growth conditions”
Claim · model-written summary
The chr19 mutation increased susceptibility to Botrytis cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- chr19 mutatione0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Gene
- chr19e0
- Outcome
- susceptibility to the JA pathway-defended necrotrophic fungal pathogen Botrytis cinereae0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“the chr19 mutation triggered higher susceptibility to the JA pathway-defended necrotrophic fungal pathogen Botrytis cinerea”
Claim · model-written summary
The chr19 mutation did not affect growth of Pseudomonas syringae pv. tomato DC3000.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- chr19 mutatione0
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e0pathogen
- Gene
- chr19e0
- Outcome
- growth of the SA pathway-defended hemibiotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000e0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“the chr19 mutation triggered higher susceptibility to the JA pathway-defended necrotrophic fungal pathogen Botrytis cinerea, but did not affect the growth of the SA pathway-defended hemibiotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000.”
Claim · model-written summary
CHR19 expression was tissue-specific.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CHR19e0
- Outcome
- Expression of CHR19 was tissue-specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Expression of CHR19 was tissue-specific”
Claim · model-written summary
SA treatment inhibited CHR19 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- SA treatmente0
- Gene
- CHR19e0
- Outcome
- Expression of CHR19e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Expression of CHR19 was tissue-specific and inhibited specifically by SA treatment.”
Claim · model-written summary
SA inhibition significantly reduced local CHR19 chromatin enrichment at associated SA pathway genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Such inhibitione0
- Gene
- CHR19e0
- Outcome
- local chromatin enrichment of CHR19 at the associated SA pathway genese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Such inhibition significantly decreased the local chromatin enrichment of CHR19 at the associated SA pathway genes”
Claim · model-written summary
SA treatment fully activated the associated SA pathway genes after CHR19 inhibition.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CHR19e0
- Outcome
- full activation upon SA treatmente0
- Qualifier
- Such inhibitione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“Such inhibition significantly decreased the local chromatin enrichment of CHR19 at the associated SA pathway genes, which resulted in their full activation upon SA treatment.”
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loss of function · Pseudomonas syringae pv. tomato str. DC3000 · ArabidopsisPositive and negative results
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See all 5 papers →loss of function · Botrytis cinerea · ArabidopsisPositive and negative results
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- positive
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.