Abstract
Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity (PTI) and disease resistance responses to different types of pathogens. It also plays a role in plant cell wall integrity as mutants impaired in the Gβ- (agb1-2) or Gγ-subunits have an altered wall composition compared with wild-type plants. Here we performed a mutant screen to identify suppressors of agb1-2 (sgb) that restore susceptibility to pathogens to wild-type levels. Out of the four sgb mutants (sgb10-sgb13) identified, sgb11 is a new mutant allele of ESKIMO1 (ESK1), which encodes a plant-specific polysaccharide O-acetyltransferase involved in xylan acetylation. Null alleles (sgb11/esk1-7) of ESK1 restore to wild-type levels the enhanced susceptibility of agb1-2 to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), but not to the bacterium Pseudomonas syringae pv. tomato DC3000 or to the oomycete Hyaloperonospora arabidopsidis. The enhanced resistance to PcBMM of the agb1-2 esk1-7 double mutant was not the result of the re-activation of deficient PTI responses in agb1-2. Alteration of cell wall xylan acetylation caused by ESK1 impairment was accompanied by an enhanced accumulation of abscisic acid, the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites and different osmolites. These esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants. These results suggest that a deficient PTI-mediated resistance is partially compensated by the activation of specific cell-wall-triggered immune responses.
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Claims 9
Claim · model-written summary
sgb11 is a new mutant allele of ESK1 in Arabidopsis
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- sgb11e2
- Gene
- ESK1e2
- Outcome
- new mutant allelee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e2“sgb11 is a new mutant allele of ESKIMO1 (ESK1), which encodes a plant-specific polysaccharide O-acetyltransferase involved in xylan acetylation”
Claim · model-written summary
Null alleles of ESK1 restored wild-type susceptibility of agb1-2 to Plectosphaerella cucumerina BMM
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Null alleles (sgb11/esk1-7) of ESK1e3
- Interactor
- Plectosphaerella cucumerina BMMPlectosphaerella cucumerinae3pathogen
- Outcome
- enhanced susceptibility of agb1-2e3
- Direction
- restoree3
- Comparator
- wild-type levelse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e3“Null alleles (sgb11/esk1-7) of ESK1 restore to wild-type levels the enhanced susceptibility of agb1-2 to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), but not to the bacterium Pseudomonas syringae pv. tomato DC3000 or to the oomycete Hyaloperonospora arabidopsidis”
Claim · model-written summary
Null alleles of ESK1 did not restore susceptibility of agb1-2 to Pseudomonas syringae DC3000 or Hyaloperonospora arabidopsidis
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Null alleles (sgb11/esk1-7) of ESK1e3
- Interactor
- Pseudomonas syringae pv. tomato DC3000Pseudomonas syringae pv. tomato str. DC3000e3pathogen
- Second interactor
- Hyaloperonospora arabidopsidis
Hyaloperonospora arabidopsidise3pathogen - Outcome
- susceptibilitye3
- Direction
- restoree3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e3“Null alleles (sgb11/esk1-7) of ESK1 restore to wild-type levels the enhanced susceptibility of agb1-2 to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), but not to the bacterium Pseudomonas syringae pv. tomato DC3000 or to the oomycete Hyaloperonospora arabidopsidis”
Claim · model-written summary
Enhanced resistance of the agb1-2 esk1-7 double mutant to PcBMM was not due to re-activation of deficient PTI responses
- Taxon
- ArabidopsisArabidopsise1
- Material
- agb1-2 esk1-7e4
- Outcome
- re-activation of deficient PTI responsese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e4“The enhanced resistance to PcBMM of the agb1-2 esk1-7 double mutant was not the result of the re-activation of deficient PTI responses in agb1-2”
Claim · model-written summary
ESK1 impairment was accompanied by enhanced accumulation of abscisic acid in Arabidopsis
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ESK1 impairmente5
- Outcome
- accumulation of abscisic acide5
- Direction
- enhancede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e5“Alteration of cell wall xylan acetylation caused by ESK1 impairment was accompanied by an enhanced accumulation of abscisic acid”
Claim · model-written summary
ESK1 impairment was associated with constitutive expression of genes encoding antibiotic peptides
- Taxon
- ArabidopsisArabidopsise1
- Gene
- genes encoding antibiotic peptidese6
- Outcome
- constitutive expressione6
- Direction
- increaseinferrede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e6“the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites and different osmolites”
Claim · model-written summary
ESK1 impairment was associated with increased accumulation of disease resistance-related secondary metabolites and different osmolites in Arabidopsis
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ESK1 impairmente5
- Outcome
- accumulation of disease resistance-related secondary metabolites and different osmolitese6
- Direction
- increaseinferrede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e5“Alteration of cell wall xylan acetylation caused by ESK1 impairment was accompanied by an enhanced accumulation of abscisic acid”
e6“the constitutive expression of genes encoding antibiotic peptides and enzymes involved in the biosynthesis of tryptophan-derived metabolites, and the accumulation of disease resistance-related secondary metabolites and different osmolites”
Claim · model-written summary
The esk1 mutation enhanced drought resistance in Arabidopsis
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- esk1e7
- Outcome
- drought resistancee7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e7“These esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants”
Claim · model-written summary
esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- esk1-mediated responsese7
- Outcome
- PcBMM susceptibility of agb1-2 plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis heterotrimeric G-protein complex modulates pathogen-associated molecular pattern-triggered immunity”
e7“These esk1-mediated responses counterbalance the defective PTI and PcBMM susceptibility of agb1-2 plants, and explain the enhanced drought resistance of esk1 plants”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato str. DC3000 · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
1 paper more in this group
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