Abstract
Since the work of Orlando Lopez-Baez in 1993, great improvements on cacao somatic embryogenesis from sporophytic tissues have been obtained. Recently, Li et al. (1998) developped a new protocole. It is now possible to produce somatic plantlets from different genotypes. After the definition of somatic embryogenesis, a bibliographic synthesis will be presented, focusing on the limitations that still exist. Cacao somatic embryogenesis has been developed at Cirad for several years. Li's protocole has been tested on many different genotypes. The efficiency of each step of the process has been determined. Histological studies were initiated in order to determine the origin of the somatic embryos. They were demonstrated to be of multicellular origin. This fact has implications for mass production and genetic transformation. RITA, a temporary immersion system developped at CIRAD, has been tested on cacao somatic embryogenesis because this system was used to improve several tissue culture processes on different plants. It significantly improved embryo production and growth. Finally, the use of cacao somatic embryogenesis in breeding programmes will be discussed, as well as the studies that need to be continued in this field. (Texte integral)
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Claims 3
Claim · model-written summary
The efficiency of each step of the cacao somatic embryogenesis process was determined.
- Taxon
- Cacao
Theobroma cacaot0 - Outcome
- efficiency of each step of the processe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives”
e1“The efficiency of each step of the process has been determined”
Claim · model-written summary
Somatic embryos of cacao were shown to be of multicellular origin.
- Taxon
- CacaoTheobroma cacaot0
- Organ / stage
- somatic embryose1
- Outcome
- origin of the somatic embryose1
- Qualifier
- multicellular origine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives”
e1“Histological studies were initiated in order to determine the origin of the somatic embryos. They were demonstrated to be of multicellular origin”
Claim · model-written summary
The RITA temporary immersion system significantly improved cacao embryo production and growth.
- Taxon
- CacaoTheobroma cacaot0
- Treatment
- RITA, a temporary immersion systeme1
- Outcome
- embryo production and growthe1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cacao somatic embryogenesis as a tool in breeding programs: statements and perspectives”
e1“RITA, a temporary immersion system developped at CIRAD, has been tested on cacao somatic embryogenesis because this system was used to improve several tissue culture processes on different plants. It significantly improved embryo production and growth”
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