Abstract
The effect of different thiourea (TU) concentrations (10 and 20 mM) on antioxidants, protease activity and protein content with and without H2O2 (50 mM) induced stress was investigated in wheat leaves. A gradual decrease in protein content with a peak at 72 hours was observed in stressed as well as control leaves. This gradual decrease in leaf protein content at different time intervals was completely prevented by 20 mM TU treatment. Leaf protease activity was increased due to oxidative stress by H2O2 while it decreased after 20 mM TU treatment under stressed and non-stressed condition. In general, catalase (CAT) activity increased under oxidative stress and after both thiourea treatments. However, at 72 hours, CAT activity reduced along with simultaneous increase in peroxidase activity under H2O2 induced stress. Actually, stress induced reduction in CAT activity at 72 hours was compensated with peroxidase. Oxidative stress and TU treatments generally raised the peroxidase activity. TU treatment followed by oxidative stress condition also increased the leaf peroxidase activity irrespective of applied concentration. Again, H2O2 and TU treatment induced reduction in CAT activity at 96 hours was compensated with a prompt increase in peroxidase activity. Collectively, H2O2 treatment increased the antioxidant and protease activities. Moreover, thiourea treatments prevented the protein loss (20mM TU), decreased the protease activity and enhanced the CAT and peroxidase activities which resulted in protective effects. Dose dependent effects of TU treatments were observed mostly.
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Claims 16
Claim · model-written summary
Leaf protein content decreased gradually over time, peaking at 72 hours, in stressed and control wheat leaves.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- leavese1
- Outcome
- protein contente1
- Direction
- decreasee1
- Qualifier
- with a peak at 72 hourse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“A gradual decrease in protein content with a peak at 72 hours was observed in stressed as well as control leaves”
Claim · model-written summary
20 mM thiourea treatment completely prevented the gradual decrease in leaf protein content.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 20 mM TU treatmente2
- Outcome
- gradual decrease in leaf protein contente2
- Direction
- completely preventede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e2“This gradual decrease in leaf protein content at different time intervals was completely prevented by 20 mM TU treatment”
Claim · model-written summary
H2O2 oxidative stress increased leaf protease activity in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- oxidative stress by H2O2e3
- Outcome
- Leaf protease activitye3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e3“Leaf protease activity was increased due to oxidative stress by H2O2”
Claim · model-written summary
20 mM thiourea treatment decreased leaf protease activity under stressed and non-stressed conditions.
- Taxon
- wheatTriticum aestivume0
- Treatment
- 20 mM TU treatmente4
- Outcome
- protease activitye4
- Direction
- decreasede4
- Qualifier
- under stressed and non-stressed conditione4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e4“Leaf protease activity was increased due to oxidative stress by H2O2 while it decreased after 20 mM TU treatment under stressed and non-stressed condition”
Claim · model-written summary
Oxidative stress increased CAT activity in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- oxidative stresse5
- Outcome
- catalase (CAT) activitye5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e5“In general, catalase (CAT) activity increased under oxidative stress and after both thiourea treatments”
Claim · model-written summary
Both thiourea treatments increased CAT activity in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- both thiourea treatmentse5
- Outcome
- catalase (CAT) activitye5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e5“In general, catalase (CAT) activity increased under oxidative stress and after both thiourea treatments”
Claim · model-written summary
Under H2O2 stress, CAT activity was reduced at 72 hours in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- H2O2 induced stresse6
- Outcome
- CAT activitye6
- Direction
- reducede6
- Qualifier
- at 72 hourse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e6“at 72 hours, CAT activity reduced along with simultaneous increase in peroxidase activity under H2O2 induced stress”
Claim · model-written summary
Under H2O2 stress, peroxidase activity increased at 72 hours in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- H2O2 induced stresse6
- Outcome
- peroxidase activitye6
- Direction
- increasee6
- Qualifier
- at 72 hourse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e6“at 72 hours, CAT activity reduced along with simultaneous increase in peroxidase activity under H2O2 induced stress”
Claim · model-written summary
Oxidative stress increased peroxidase activity in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Oxidative stresse7
- Outcome
- peroxidase activitye7
- Direction
- raisede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e7“Oxidative stress and TU treatments generally raised the peroxidase activity”
Claim · model-written summary
Thiourea treatments increased peroxidase activity in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TU treatmentse7
- Outcome
- peroxidase activitye7
- Direction
- raisede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e7“Oxidative stress and TU treatments generally raised the peroxidase activity”
Claim · model-written summary
Thiourea treatment followed by oxidative stress increased leaf peroxidase activity regardless of concentration.
- Taxon
- wheatTriticum aestivume0
- Treatment
- TU treatment followed by oxidative stress conditione1
- Outcome
- leaf peroxidase activitye1
- Direction
- increasede1
- Qualifier
- irrespective of applied concentratione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“TU treatment followed by oxidative stress condition also increased the leaf peroxidase activity irrespective of applied concentration”
Claim · model-written summary
H2O2 and thiourea treatment reduced CAT activity at 96 hours in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- H2O2 and TU treatmente1
- Outcome
- CAT activitye1
- Direction
- reductione1
- Qualifier
- at 96 hourse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“H2O2 and TU treatment induced reduction in CAT activity at 96 hours was compensated with a prompt increase in peroxidase activity”
Claim · model-written summary
H2O2 and thiourea treatment increased peroxidase activity at 96 hours in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- H2O2 and TU treatmente1
- Outcome
- peroxidase activitye1
- Direction
- prompt increasee1
- Qualifier
- at 96 hourse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“H2O2 and TU treatment induced reduction in CAT activity at 96 hours was compensated with a prompt increase in peroxidase activity”
Claim · model-written summary
H2O2 treatment increased antioxidant and protease activities in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Treatment
- H2O2 treatmente1
- Outcome
- antioxidant and protease activitiese1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“H2O2 treatment increased the antioxidant and protease activities”
Claim · model-written summary
Thiourea treatments enhanced CAT and peroxidase activities, which resulted in protective effects.
- Taxon
- wheatTriticum aestivume0
- Treatment
- thiourea treatmentse1
- Outcome
- CAT and peroxidase activitiese1
- Direction
- enhancede1
- Qualifier
- resulted in protective effectse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“thiourea treatments prevented the protein loss (20mM TU), decreased the protease activity and enhanced the CAT and peroxidase activities which resulted in protective effects”
Claim · model-written summary
Thiourea effects on wheat leaves were mostly dose dependent.
- Taxon
- wheatTriticum aestivume0
- Outcome
- effects of TU treatmentse1
- Qualifier
- Dose dependente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was investigated in wheat leaves”
e1“Dose dependent effects of TU treatments were observed mostly”
The same questions in other papers
thiourea · physiology: antioxidant enzymes · Triticum aestivum (wheat)Results differ
6 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
5 papers more in this group
See all 9 papers →hydrogen peroxide · physiology: antioxidant enzymes · Triticum aestivum (wheat)Results differ
13 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
12 papers more in this group
See all 16 papers →thiourea · quality: protein · Triticum aestivum (wheat)Results agree
1 paper in the same direction as this paper
- positive
oxidative · physiology: antioxidant enzymes · Triticum aestivum (wheat)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.