Abstract
Lilium ledebourii (Baker) Boiss. (Liliaceae) is a critically endangered lily species native tonorthern Iran, where it is protected by law. In order to develop a cost effective method for largescalepropagation, the effects of three culture systems (solid, liquid and temporary immersion)and two types of cytokinins [6-Benzyladenine (BA) and Thidiazuron (TDZ)] were studied onthe in vitro plant regeneration of L. ledebourii. To establish the protocol, we used in vitroregenerated bulblets obtained from bulb scale segments that were cultured on solid Murashigeand Skoog (MS) media as starting material. The bulblet microscale transverse thin cell layerswere cultured on MS solid medium containing 3% sucrose and different combinations of plantgrowth regulators. Choice of both, the culture system and the type of cytokinin, affected thedifferentiation of explants. Two types of calli formed on explants: type I callus wasembryogenic, while type II callus was shoot organogenesis. The highest percentage (94%) ofembryogenic callus was obtained when calli were transferred on MS solid media supplementedwith 0.54 μM α-Naphthaleneacetic acid (NAA) and 0.44 μM BA. In addition, it was alsoobserved that the use of temporary immersion bioreactor resulted in a significantly loweramount of shoot organogenesis rather than solid culture systems. Seventy percent of theplantlets were successfully acclimatized to ex–vitro conditions and were phenotypically similarto the mother plants.
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Claims 6
Claim · model-written summary
Both the culture system and the type of cytokinin affected the differentiation of Lilium ledebourii explants.
- Taxon
- Lilium ledebourii
Lilium ledebouriit0 - Treatment
- the culture system and the type of cytokinine0
- Outcome
- differentiation of explantse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Choice of both, the culture system and the type of cytokinin, affected the differentiation of explants”
t0“Lilium ledebourii”
Claim · model-written summary
Two types of calli formed on explants: type I was embryogenic and type II was shoot organogenesis.
- Taxon
- Lilium ledebouriiLilium ledebouriit0
- Outcome
- Two types of calli formed on explantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two types of calli formed on explants: type I callus wasembryogenic, while type II callus was shoot organogenesis”
t0“Lilium ledebourii”
Claim · model-written summary
The highest percentage (94%) of embryogenic callus was obtained on MS solid medium supplemented with 0.54 μM NAA and 0.44 μM BA.
- Taxon
- Lilium ledebouriiLilium ledebouriit0
- Treatment
- MS solid media supplementedwith 0.54 μM α-Naphthaleneacetic acid (NAA) and 0.44 μM BAe0
- Outcome
- embryogenic calluse0
- Direction
- higheste0
- Value
- 94%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage (94%) ofembryogenic callus was obtained when calli were transferred on MS solid media supplementedwith 0.54 μM α-Naphthaleneacetic acid (NAA) and 0.44 μM BA”
t0“Lilium ledebourii”
Claim · model-written summary
The temporary immersion bioreactor gave a significantly lower amount of shoot organogenesis than solid culture systems.
- Taxon
- Lilium ledebouriiLilium ledebouriit0
- Treatment
- temporary immersion bioreactore0
- Outcome
- shoot organogenesise0
- Direction
- lowere0
- Comparator
- solid culture systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the use of temporary immersion bioreactor resulted in a significantly loweramount of shoot organogenesis rather than solid culture systems”
t0“Lilium ledebourii”
Claim · model-written summary
Seventy percent of the plantlets were successfully acclimatized to ex-vitro conditions.
- Taxon
- Lilium ledebouriiLilium ledebouriit0
- Outcome
- successfully acclimatized to ex–vitro conditionse0
- Value
- Seventy percente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seventy percent of theplantlets were successfully acclimatized to ex–vitro conditions”
t0“Lilium ledebourii”
Claim · model-written summary
The acclimatized plantlets were phenotypically similar to the mother plants.
- Taxon
- Lilium ledebouriiLilium ledebouriit0
- Outcome
- phenotypically similarto the mother plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were phenotypically similarto the mother plants”
t0“Lilium ledebourii”
The same questions in other papers
N-benzyladenine · growth · Lilium ledebouriiResults agree
2 papers in the same direction as this paper
- positive
- positive
1-naphthaleneacetic acid · growth · Lilium ledebouriiResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · growth · Lilium ledebouriiPositive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.