Claim · model-written summary
NLN medium gave the highest callus induction rate (28.57 %) in L. ledebourii anther culture, above B5 and MS.
Verbatim
e1“NLN . yielded the highest rate of callus induction (28.57 %), followed by B5 (24.06 %), with MS medium yielding the lowest (13.41 %)”
t0“Lilium ledebourii”
Title
Haploid plant production and flowcytometric evaluation of Lilium ledebourii (Baker) Boiss
Mohsen Bagherian-Shamasbi; Esmaeil Chamani; Younes Pourbeyrami Hir; et al. Scientia Horticulturae 2025. doi:10.1016/j.scienta.2025.113950
Other claims from this paper (8)
- Intervention effect B5 medium gave a callus induction rate of 24.06 %, second to NLN and above MS.
- Intervention effect MS medium gave the lowest callus induction rate (13.41 %).
- Intervention effect NLN medium yielded the largest callus size, followed by B5, while MS medium yielded the smallest.
- Intervention effect 1 mg/L 2,4-D with 1 mg/L TDZ was the optimal combination for callogenesis, giving a callus induction of 79.68 %.
- Intervention effect The optimal 2,4-D and TDZ combination gave a callus size of 44.4 mm.
- Intervention effect The highest embryogenesis percentage (76.65 %) was attained with 2 mg/L Picloram and 1 mg/L Kinetin.
- Observation Among regenerated bulblets, three were haploid and the remainder were diploid, like the control, by chromosome count and flow cytometry.
- Recommendation The authors conclude that the anther culture protocol holds promise for producing homozygous L. ledebourii plants.
