Abstract
Ascorbate peroxidase (APX) plays a central role in the ascorbate-glutathione cycle and is a key enzyme in cellular H2O2 me-tabolism. It includes a family of isoenzymes with different character-istics, which are identified in many higher plants. In the present study, we isolated the APX gene from Jatropha curcas L, which is similar with other previously characterized APXs as revealed by alignment and phylogenetic analysis of its deduced amino acid sequence. Real-time qPCR analysis showed that the expression level of JcAPX transcript significantly increased under NaCl stress. Subsequently, to elucidate the contribution of JcAPX to the protection against salt-induced oxi-dative stress, the expression construct p35S: JcAPX was created and transformed into Arabidopsis and transcribed. Under 150-mM NaCl stress, compared with wild type (WT), the overexpression of JcAPX in Arabidopsis increased the germination rate, the number of leaves, and the rosette area. In addition, the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions. These results suggested that higher APX activity in transgenic lines increases the salt tolerance by enhancing scavenging capacity for reactive oxygen spe-cies under NaCl stress conditions.
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Claims 7
Claim · model-written summary
The isolated Jatropha APX resembled previously characterized APXs.
- Taxon
- Jatropha curcas L
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Gene
- APXe0
- Outcome
- similar with other previously characterized APXse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“we isolated the APX gene from Jatropha curcas L, which is similar with other previously characterized APXs”
Claim · model-written summary
JcAPX transcript increased under NaCl stress.
- Taxon
- Jatropha curcasJatropha curcast0
- Treatment
- NaCl stresse0
- Gene
- JcAPXe0
- Outcome
- expression level of JcAPX transcripte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression level of JcAPX transcript significantly increased under NaCl stress”
t0“Jatropha curcas”
Claim · model-written summary
JcAPX overexpression increased germination rate, leaf number and rosette area under salt stress.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of JcAPXe0
- Gene
- JcAPXe0
- Outcome
- germination rate, the number of leaves, and the rosette areae0
- Direction
- increasede0
- Qualifier
- 150-mM NaCl stresse0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under 150-mM NaCl stress, compared with wild type (WT), the overexpression of JcAPX in Arabidopsis increased the germination rate, the number of leaves, and the rosette area”
Claim · model-written summary
Transgenic plants had longer roots under NaCl stress than WT.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic plantse0
- Outcome
- rootse0
- Direction
- longere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions”
t0“Arabidopsis”
Claim · model-written summary
Transgenic plants had higher total chlorophyll content than WT under NaCl stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic plantse0
- Outcome
- total chlorophyll contente0
- Direction
- highere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions”
t0“Arabidopsis”
Claim · model-written summary
Transgenic plants had higher total APX activity than WT under NaCl stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic plantse0
- Outcome
- total APX activitye0
- Direction
- highere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions”
t0“Arabidopsis”
Claim · model-written summary
Transgenic plants had lower H2O2 content than WT under NaCl stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- transgenic plantse0
- Outcome
- H2O2 contente0
- Direction
- lowere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions”
t0“Arabidopsis”
The same questions in other papers
salinity · molecular: expression · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.