Abstract
Although the SnRK2 class of Ser/Thr protein kinases is critical for signal transduction and abiotic stress resistance in plants, there have been no studies to examine SnRK2 in Jatropha curcas L. In the present study, JcSnRK2 was cloned from J. curcas using the rapid amplification of cDNA end technique and characterized. The JcSnRK2 genomic sequence is 2578 base pairs (bp), includes 10 exons and 9 introns, and the 1017-bp open reading frame encodes 338 amino acids. JcSnRK2 was transcribed in all examined tissues, with the highest transcription rate observed in the roots, followed by the leaves and stalks; the lowest rate was observed in flowers and seeds. JcSnRK2 expression increased following abscisic acid treatment, salinity, and drought stress. During a 48-h stress period, the expression of JcSnRK2 showed 2 peaks and periodic up- and downregulation. JcSnRK2 was rapidly activated within 1 h under salt and drought stress, but not under cold stress. Because of the gene sequence and expression similarity of JcSnRK2 to AtSnRK2.8, primarily in the roots, an eukaryotic expression vector containing the JcSnRK2 gene (pBI121-JcSnRK2) was constructed and introduced to the Arabidopsis AtSnRK2.8 mutant snf2.8. JcSnRK2-overexpressing plants exhibited higher salt and drought tolerance, further demonstrating the function of JcSnRK2 in the osmotic stress response. J. curcas is highly resistant to extreme salt and drought conditions and JcSnRK2 was found to be activated under these conditions. Thus, JcSnRK2 is potential candidate for improving crop tolerance to salt and drought stress.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
JcSnRK2 was cloned from Jatropha.
- Taxon
- J. curcas
_tree_at_Samedha.jpg?width=60)
Jatropha curcase0 - Gene
- JcSnRK2e0
- Outcome
- clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“JcSnRK2 was cloned from J. curcas”
Claim · model-written summary
The JcSnRK2 genomic sequence spans 2578 base pairs.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Gene
- JcSnRK2e0
- Outcome
- genomic sequencee0
- Value
- 2578 base pairs (bp)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The JcSnRK2 genomic sequence is 2578 base pairs (bp)”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 contains 10 exons and 9 introns.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- exons and 9 intronse0
- Value
- 10 exons and 9 intronse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“includes 10 exons and 9 introns”
t0“Jatropha curcas L.”
Claim · model-written summary
The JcSnRK2 open reading frame is 1017 bp and encodes 338 amino acids.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- open reading framee0
- Value
- 1017-bpe0
- Qualifier
- 338 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 1017-bp open reading frame encodes 338 amino acids”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 was transcribed in all examined tissues, with highest transcription in roots and lowest in flowers and seeds.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Organ / stage
- rootse0
- Gene
- JcSnRK2e0
- Outcome
- transcription ratee0
- Direction
- higheste0
- Qualifier
- lowest rate was observed in flowers and seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 was transcribed in all examined tissues, with the highest transcription rate observed in the roots, followed by the leaves and stalks; the lowest rate was observed in flowers and seeds”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 expression increased after abscisic acid treatment.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- abscisic acid treatmente0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 expression increased following abscisic acid treatment”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 expression increased under salinity.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- salinitye0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 expression increased following abscisic acid treatment, salinity, and drought stress”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 expression increased under drought stress.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- drought stresse0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2 expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 expression increased following abscisic acid treatment, salinity, and drought stress”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 expression had two peaks with periodic up- and downregulation over 48 hours.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Gene
- JcSnRK2e0
- Outcome
- expressione0
- Value
- 2 peakse0
- Qualifier
- 48-h stress periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During a 48-h stress period, the expression of JcSnRK2 showed 2 peaks and periodic up- and downregulation”
t0“Jatropha curcas L.”
Claim · model-written summary
Salt stress activated JcSnRK2 within one hour.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- salte0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2e0
- Direction
- activatede0
- Value
- within 1 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 was rapidly activated within 1 h under salt and drought stress, but not under cold stress”
t0“Jatropha curcas L.”
Claim · model-written summary
Drought stress activated JcSnRK2 within one hour.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- drought stresse0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2e0
- Direction
- activatede0
- Value
- within 1 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 was rapidly activated within 1 h under salt and drought stress, but not under cold stress”
t0“Jatropha curcas L.”
Claim · model-written summary
Cold stress did not activate JcSnRK2.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- cold stresse0
- Gene
- JcSnRK2e0
- Outcome
- JcSnRK2e0
- Direction
- not under cold stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 was rapidly activated within 1 h under salt and drought stress, but not under cold stress”
t0“Jatropha curcas L.”
Claim · model-written summary
JcSnRK2 overexpression increased salt tolerance in Arabidopsis mutant plants.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- JcSnRK2-overexpressinge0
- Gene
- JcSnRK2e0
- Outcome
- salte0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2-overexpressing plants exhibited higher salt and drought tolerance”
e1“Arabidopsis AtSnRK2.8 mutant snf2.8”
Claim · model-written summary
JcSnRK2 overexpression increased drought tolerance in Arabidopsis mutant plants.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- JcSnRK2-overexpressinge0
- Gene
- JcSnRK2e0
- Outcome
- drought tolerancee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2-overexpressing plants exhibited higher salt and drought tolerance”
e1“Arabidopsis AtSnRK2.8 mutant snf2.8”
Claim · model-written summary
The authors propose JcSnRK2 as a candidate for improving crop salt and drought tolerance.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Gene
- JcSnRK2e0
- Outcome
- improving crop tolerance to salt and drought stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JcSnRK2 is potential candidate for improving crop tolerance to salt and drought stress”
t0“Jatropha curcas L.”
The same questions in other papers
drought · molecular: expression · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
salinity · molecular: expression · Jatropha curcasResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.