Abstract
This study was undertaken to test the effect of genotype, explant type, growth regulator, explant age and gelling agents on cotton callus induction. Callus cultures were initiated from the cotyledon and hypocotyls explants of the three cotton (Gossypium hirsutum L.) cultivars Coker 310, MCU 12 and KC 3 on MS basal medium supplemented with different concentrations (0.1, 0.5, 1.0 mg L−1) and combinations of growth regulators (2, 4-D and Kinetin, 2, 4-D and BAP, 2, 4-D and TDZ and 2, 4-D and Zeatin). Of the thirty six different callus induction medium (CIM) tried, CIM2 medium supplemented with 0.1mg L−1 2, 4-D and 0.5mg L−1 kinetin promoted 100% callus induction irrespective of the genotypes and explants. It was also observed that callus initiation was found to be quicker in hypocotyls than cotyledon explants. Of the different explant ages (4, 7, 10, 14, 21 and 28 days), 7 day old cotyledon and hypocotyls explants exhibited highest callus induction frequency irrespective of the genotypes. Out of the two gelling agents compared, the rate of callus proliferation was found to be faster with 0.4% phytagel than with 0.8% agar. Thus, conditions for initiation of callus in cotton (Gossypium hirsutum L.) cultivars Coker 310, MCU 12 and KC 3 were optimized in this study.
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Claims 4
Claim · model-written summary
CIM2 with 0.1 mg/L 2,4-D and 0.5 mg/L kinetin produced 100% callus induction across genotypes and explants.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- CIM2 medium supplemented with 0.1mg L−1 2, 4-D and 0.5mg L−1 kinetine0
- Outcome
- callus inductione0
- Direction
- promotede0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CIM2 medium supplemented with 0.1mg L−1 2, 4-D and 0.5mg L−1 kinetin promoted 100% callus induction irrespective of the genotypes and explants”
t0“cotton”
Claim · model-written summary
Callus initiated sooner in hypocotyl than cotyledon explants.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- hypocotylse0
- Outcome
- callus initiatione0
- Direction
- quickere0
- Comparator
- cotyledon explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus initiation was found to be quicker in hypocotyls than cotyledon explants”
t0“cotton”
Claim · model-written summary
Seven-day-old cotyledon and hypocotyl explants had the highest callus induction frequency across genotypes.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- cotyledon and hypocotyls explantse0
- Outcome
- callus induction frequencye0
- Direction
- higheste0
- Value
- 7 daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 day old cotyledon and hypocotyls explants exhibited highest callus induction frequency irrespective of the genotypes”
t0“cotton”
Claim · model-written summary
Callus proliferated faster on 0.4% phytagel than on 0.8% agar.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- 0.4% phytagele0
- Outcome
- rate of callus proliferatione0
- Direction
- fastere0
- Comparator
- 0.8% agare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rate of callus proliferation was found to be faster with 0.4% phytagel than with 0.8% agar”
t0“cotton”
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.