Abstract
감귤에 있어서 원형질체 융합에 의한 체세포잡종체 생산을 위해서는 우선 캘러스 원형질체로부터의 식물체 재분화가 가능하여야 하기 때문에 온주밀감의 캘러스 원형질체로 부터 배형성과정을 통한 식물체 재분화에 관한 실험을 수행하였다. 흥진조생의 어린 미성숙 배주의 주심조직으로부터 유기된 배형성 캘러스를 사용하여 건강한 원형질체를 분리하고 0.6 M $BH_3$ 배지를 배양배지로 사용하여 배양방법에 따른 plating efficiency를 비교해 본 결과 liquid over solid 배양시 원형질체로부터의 미소괴 형성율이 더 높음을 알 수 있었으며, 적정 배형성 배지 선발에서는 1500 mg/L malt extract 첨가 배지에서 배형성율이 높았다. 자엽형 배로부터의 신초유기를 위한 배지비교에서는 1.0 mg/L GAB 첨가배지에서 발근과 함께 정상적으로 재분화된 신초를 얻을 수 있었다. 원형질체 배양으로부터 재분화된 식물체들은 순화과정을 거쳐 온실 육묘중에 있으며, 생장상이나 형태적인 특성에 있어서 모본 식물체와 차이가 없음을 확인할 수 있었다. 본 실험으로 얻어진 원형질체 배양에 의한 식물체 재분화 체계를 바탕으로 이종속간 감귤의 원형질체 융합 기술을 이용하여 교배육종이 불가능한 품종들로부터 우수한 형질을 지닌 감귤 품종 및 대목용 품종 생산에 활용하고자 한다. This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M $BH_3$ medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.
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Claims 5
Claim · model-written summary
The liquid over solid method was most effective for microcallus formation.
- Taxon
- satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)
Citrus unshiue1 - Treatment
- liquid over solid methode0
- Outcome
- formation of microcallie0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The liquid over solid method was the most effective for formation of microcalli”
e1“satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)”
Claim · model-written summary
Most microcalli grew rapidly.
- Taxon
- satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)Citrus unshiue1
- Outcome
- microcalli grew rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of microcalli grew rapidly”
e1“satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)”
Claim · model-written summary
MT medium with malt extract and sucrose was optimal for embryoid formation.
- Taxon
- satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)Citrus unshiue1
- Treatment
- MT medium containing 1.5 g/L malt extract, 0.146 M sucrosee0
- Outcome
- embryoid formatione0
- Direction
- Optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose”
e1“satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)”
Claim · model-written summary
MS medium with sucrose and GA3 was the medium for plantlet regeneration.
- Taxon
- satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)Citrus unshiue1
- Treatment
- MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$e0
- Outcome
- plantlet regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L $GA_3$”
e1“satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)”
Claim · model-written summary
Protoplast-derived plants and nucellar seedlings had similar growth habits and leaf characters.
- Taxon
- satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)Citrus unshiue1
- Outcome
- growth habits and leaf characters such as shape, thickness, and coloure0
- Direction
- No differencese0
- Comparator
- nucellar seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings”
e1“satsuma mandarin cv. Okitsu (Citrus unshiu Marc.)”
The same questions in other papers
in vitro · growth · Citrus unshiu (satsuma mandarin)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.