Abstract
To better understand the function of melatonin in tomato fruit ripening and quality improvement, a label-free quantitation method was used to investigate the proteins that differ between the control (CK) and 50 μm melatonin treatment (M50) fruits. Proteomics data identified 241 proteins that were significantly influenced by melatonin. These proteins were involved in several ripening-related pathways, including cell wall metabolism, oxidative phosphorylation, carbohydrate, and fatty acid metabolism. Moreover, the application of exogenous melatonin increased eight proteins that are related to anthocyanin accumulation during fruit ripening. Additionally, the affected protein levels correlated with the corresponding gene transcript levels. Further, the total anthocyanin content from M50 increased by 52%, 48%, and 50% at 5, 8, and 13 DAT (day after melatonin treatment), respectively. The melatonin-mediated promotion of fruit ripening and quality might be due to the altered proteins involved in processes associated with ripening. In this work, we indicated that a senescence-related protein was downregulated in the M50 fruit, while a cell apoptosis inhibitor (API5) protein was upregulated. In addition, peroxidases (POD9, POD12, peroxidase p7-like) and catalase (CAT3) significantly increased in the M50 fruits. Based on the previous studies and our data, we inferred that melatonin might be positively related to fruit ripening but negatively related to fruit senescence. This research provides insights into the physiological and molecular mechanisms underlying melatonin-mediated fruit ripening as well as the anthocyanin formation process in tomato fruit at the protein concentration level, and we reveal possible candidates for regulation of anthocyanin formation during fruit ripening.
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Claims 13
Claim · model-written summary
Melatonin significantly influenced 241 proteins.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- melatonine0
- Outcome
- proteinse0
- Value
- 241 proteinse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“Proteomics data identified 241 proteins that were significantly influenced by melatonin.”
Claim · model-written summary
Affected proteins were involved in multiple ripening related pathways.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- cell wall metabolism, oxidative phosphorylation, carbohydrate, and fatty acid metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“These proteins were involved in several ripening-related pathways, including cell wall metabolism, oxidative phosphorylation, carbohydrate, and fatty acid metabolism.”
Claim · model-written summary
Melatonin increased eight anthocyanin related proteins.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- exogenous melatonine0
- Outcome
- proteins that are related to anthocyanin accumulatione0
- Direction
- increasede0
- Value
- eight proteinse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the application of exogenous melatonin increased eight proteins that are related to anthocyanin accumulation during fruit ripening.”
Claim · model-written summary
Affected protein levels correlated with corresponding transcript levels.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- corresponding gene transcript levelse0
- Outcome
- affected protein levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the affected protein levels correlated with the corresponding gene transcript levels.”
Claim · model-written summary
Melatonin increased total anthocyanin content by 52% at 5 DAT.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Treatment
- melatonin treatmente0
- Outcome
- total anthocyanin contente0
- Direction
- increasede0
- Value
- 52%e0
- Qualifier
- 5e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the total anthocyanin content from M50 increased by 52%, 48%, and 50% at 5, 8, and 13 DAT (day after melatonin treatment), respectively.”
Claim · model-written summary
Melatonin increased total anthocyanin content by 48% at 8 DAT.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Treatment
- melatonin treatmente0
- Outcome
- total anthocyanin contente0
- Direction
- increasede0
- Value
- 48%e0
- Qualifier
- 8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the total anthocyanin content from M50 increased by 52%, 48%, and 50% at 5, 8, and 13 DAT (day after melatonin treatment), respectively.”
Claim · model-written summary
Melatonin increased total anthocyanin content by 50% at 13 DAT.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Treatment
- melatonin treatmente0
- Outcome
- total anthocyanin contente0
- Direction
- increasede0
- Value
- 50%e0
- Qualifier
- 13 DATe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the total anthocyanin content from M50 increased by 52%, 48%, and 50% at 5, 8, and 13 DAT (day after melatonin treatment), respectively.”
Claim · model-written summary
A senescence related protein was downregulated under melatonin.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Gene
- senescence-related proteine0
- Outcome
- senescence-related proteine0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“a senescence-related protein was downregulated in the M50 fruit, while a cell apoptosis inhibitor (API5) protein was upregulated.”
Claim · model-written summary
API5 protein was upregulated under melatonin.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Gene
- API5e0
- Outcome
- API5) proteine0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“a senescence-related protein was downregulated in the M50 fruit, while a cell apoptosis inhibitor (API5) protein was upregulated.”
Claim · model-written summary
POD9, POD12 and peroxidase p7-like proteins significantly increased in treated fruit.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- M50e0
- Gene
- POD9, POD12, peroxidase p7-likee0
- Outcome
- peroxidasese0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“peroxidases (POD9, POD12, peroxidase p7-like) and catalase (CAT3) significantly increased in the M50 fruits.”
Claim · model-written summary
CAT3 significantly increased in treated fruit.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“peroxidases (POD9, POD12, peroxidase p7-like) and catalase (CAT3) significantly increased in the M50 fruits.”
Claim · model-written summary
The authors inferred that melatonin is positively related to ripening.
- Taxon
- tomatoSolanum lycopersicumt0
- Compound
- melatonine0
- Outcome
- fruit ripeninge0
- Direction
- positively relatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“we inferred that melatonin might be positively related to fruit ripening but negatively related to fruit senescence.”
Claim · model-written summary
The authors inferred that melatonin is negatively related to senescence.
- Taxon
- tomatoSolanum lycopersicumt0
- Compound
- melatonine0
- Outcome
- fruit senescencee0
- Direction
- negatively relatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“we inferred that melatonin might be positively related to fruit ripening but negatively related to fruit senescence.”
The same questions in other papers
melatonin · quality: phytochemicals · Solanum lycopersicum (tomato)Results agree
11 papers in the same direction as this paper
- positive
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8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.