Abstract
The effects of natural ventilation and CO 2 enrichment during the rooting stage on the growth and the rates of photosynthesis and transpiration of in vitro cauliflower ( Brassica oleracea L.) plantlets were investigated. In vitro plantlets were established in airtight or ventilated vessels with or without CO 2 supplied (≈1200 μg·L -1 ) through gas permeable films attached to the vessel's cap for 15 days before transplanting ex vitro. Leaves generated in vitro in ventilated vessels had a higher photosynthetic rate than those produced in airtight vessels, which lead to greater leaf expansion and shoot and root dry matter accumulation during in vitro culture and acclimatization. Enhanced photosynthesis in leaves of ventilated plantlets was positively correlated with chlorophyll content. Increasing photosynthetically active radiation from 70 to 200 μmol·m -2 ·s -1 enhanced the growth of in vitro plantlets under ventilated conditions but it depressed photosynthesis of the leaves grown photomixotrophically with sugar and CO 2 enrichment which might be due to the feedback inhibition caused by marked accumulations of sucrose and starch. Higher CO 2 levels during in vitro culture enhanced photosynthesis under photoautotrophic conditions, but inhibited it under photomixotrophic conditions. Fifteen days after transplanting ex vitro, high photosynthetic ability and stomatal resistance to transpiratory water loss of ventilated plantlets in vitro had important contributions to rooting and acclimatization. Our findings show that the ventilated culture is effective for accelerating photoautotrophic growth of plantlets by increasing photosynthesis, suggesting that, especially for plantlets growing in vitro without sugar, CO 2 enrichment may be necessary to enhance photosynthetic ability.
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Claims 10
Claim · model-written summary
Ventilated vessels produced leaves with higher photosynthetic rates than airtight vessels.
- Taxon
- cauliflower
Brassica oleracea var. botrytist0 - Organ / stage
- Leavese0
- Treatment
- ventilated vesselse0
- Outcome
- photosynthetic ratee0
- Direction
- highere0
- Comparator
- airtight vesselse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaves generated in vitro in ventilated vessels had a higher photosynthetic rate than those produced in airtight vessels”
t0“Cauliflower”
Claim · model-written summary
Ventilated vessels increased leaf expansion and shoot and root dry matter accumulation.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- ventilated vesselse1
- Outcome
- leaf expansion and shoot and root dry matter accumulatione0
- Direction
- greatere0
- Comparator
- airtight vesselse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which lead to greater leaf expansion and shoot and root dry matter accumulation during in vitro culture and acclimatization.”
t0“Cauliflower”
e1“Leaves generated in vitro in ventilated vessels had a higher photosynthetic rate than those produced in airtight vessels”
Claim · model-written summary
Photosynthesis in ventilated plantlets correlated positively with chlorophyll content.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Compound
- chlorophylle0
- Outcome
- photosynthesis in leaves of ventilated plantletse0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced photosynthesis in leaves of ventilated plantlets was positively correlated with chlorophyll content.”
t0“Cauliflower”
Claim · model-written summary
Raising radiation from 70 to 200 μmol·m -2 ·s -1 enhanced growth under ventilated conditions.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- Increasing photosynthetically active radiation from 70 to 200 μmol·m -2 ·s -1e0
- Outcome
- growth of in vitro plantletse0
- Direction
- enhancede0
- Comparator
- 70e0
- Setting
- ventilated conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing photosynthetically active radiation from 70 to 200 μmol·m -2 ·s -1 enhanced the growth of in vitro plantlets under ventilated conditions”
t0“Cauliflower”
Claim · model-written summary
Higher radiation depressed photosynthesis in photomixotrophic leaves with sugar and CO 2 enrichment.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- Increasing photosynthetically active radiatione1
- Outcome
- photosynthesis of the leavese0
- Direction
- depressede0
- Qualifier
- photomixotrophically with sugar and CO 2 enrichmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it depressed photosynthesis of the leaves grown photomixotrophically with sugar and CO 2 enrichment”
t0“Cauliflower”
e1“Increasing photosynthetically active radiation from 70 to 200 μmol·m -2 ·s -1 enhanced the growth of in vitro plantlets under ventilated conditions”
Claim · model-written summary
Higher CO 2 enhanced photosynthesis under photoautotrophic conditions.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- Higher CO 2 levelse0
- Outcome
- photosynthesise0
- Direction
- enhancede0
- Qualifier
- photoautotrophic conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher CO 2 levels during in vitro culture enhanced photosynthesis under photoautotrophic conditions”
t0“Cauliflower”
Claim · model-written summary
Higher CO 2 inhibited photosynthesis under photomixotrophic conditions.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- Higher CO 2 levelse0
- Outcome
- photosynthesise0
- Direction
- inhibitede0
- Qualifier
- photomixotrophic conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher CO 2 levels during in vitro culture enhanced photosynthesis under photoautotrophic conditions, but inhibited it under photomixotrophic conditions.”
t0“Cauliflower”
Claim · model-written summary
After transplanting, high photosynthesis and stomatal resistance contributed to rooting and acclimatization.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- rooting and acclimatizatione0
- Qualifier
- high photosynthetic ability and stomatal resistance to transpiratory water losse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifteen days after transplanting ex vitro, high photosynthetic ability and stomatal resistance to transpiratory water loss of ventilated plantlets in vitro had important contributions to rooting and acclimatization.”
t0“Cauliflower”
Claim · model-written summary
The authors conclude ventilated culture accelerates photoautotrophic plantlet growth by increasing photosynthesis.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Treatment
- ventilated culturee0
- Outcome
- photoautotrophic growth of plantletse0
- Qualifier
- by increasing photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ventilated culture is effective for accelerating photoautotrophic growth of plantlets by increasing photosynthesis”
t0“Cauliflower”
Claim · model-written summary
The authors suggest CO 2 enrichment may be needed to enhance photosynthesis for in vitro plantlets without sugar.
- Taxon
- cauliflowerBrassica oleracea var. botrytist0
- Outcome
- CO 2 enrichment may be necessary to enhance photosynthetic abilitye0
- Qualifier
- plantlets growing in vitro without sugare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“especially for plantlets growing in vitro without sugar, CO 2 enrichment may be necessary to enhance photosynthetic ability.”
t0“Cauliflower”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.