Abstract
Abstract Callus from leaf explants of two pomegranate ( Punica granatum L.) clones produced adventitious shoots after 2 months of in vitro culture. Leaf segment explants initially were cultured on a modified Murashige and Skoog (MS) basal medium supplemented with a range of 0.5-10 μ m BA and 0.05-5 (μ m NAA. Shoot elongation was stimulated most efficiently when the initial calli were transferred from the shoot induction medium to half-strength MS supplemented with 2 μ m BA. Elongated shoots rooted easily on a medium of one-half MS and 0.1 μ m NAA. Whole plants were recovered after transfer to vermiculite in a greenhouse. Chemical names used: N- (phenylmethyl)-1 H -purin-6-amine (BA), 1-naphthaleneacetic acid (NAA).
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Claims 4
Claim · model-written summary
Callus from leaf explants of two pomegranate clones produced adventitious shoots after two months in vitro.
- Taxon
- pomegranate ( Punica granatum L.)
Punica granatume0 - Material
- two pomegranate ( Punica granatum L.) clonese0
- Organ / stage
- leaf explantse0
- Outcome
- adventitious shootse0
- Value
- 2 monthse0
- Setting
- in vitro culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus from leaf explants of two pomegranate ( Punica granatum L.) clones produced adventitious shoots after 2 months of in vitro culture.”
Claim · model-written summary
Transfer to half strength MS with 2 μ m BA stimulated shoot elongation most efficiently.
- Taxon
- PomegranatePunica granatumt0
- Organ / stage
- initial callie0
- Treatment
- half-strength MS supplemented with 2 μ m BAe0
- Outcome
- Shoot elongatione0
- Direction
- stimulated most efficientlye0
- Comparator
- shoot induction mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot elongation was stimulated most efficiently when the initial calli were transferred from the shoot induction medium to half-strength MS supplemented with 2 μ m BA.”
t0“Pomegranate”
Claim · model-written summary
Elongated shoots rooted easily on half MS with 0.1 μ m NAA.
- Taxon
- PomegranatePunica granatumt0
- Organ / stage
- Elongated shootse0
- Treatment
- one-half MS and 0.1 μ m NAAe0
- Outcome
- rooted easilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elongated shoots rooted easily on a medium of one-half MS and 0.1 μ m NAA.”
t0“Pomegranate”
Claim · model-written summary
Whole pomegranate plants were recovered after transfer to vermiculite in a greenhouse.
- Taxon
- PomegranatePunica granatumt0
- Treatment
- transfer to vermiculitee0
- Outcome
- Whole plants were recoverede0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whole plants were recovered after transfer to vermiculite in a greenhouse.”
t0“Pomegranate”
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