Abstract
Changes in carbohydrate and nitrogen fractions during seed germination of fenugreek under water stress and GA^ treatment were investigated. The germination percentage was reduced with the decrease in the osmotic potential of seed media, reaching its lower value at -0.8 MPa. Sucrose was accumulated considerably which could have had a major contribution in the total carbohydrate, while monosaccharides showed a substantial reduction and polysaccharides demonstrated no consistent pattern of changes due to water stress. The response of total nitrogen was variable; it increased considerably in -0.1 and -0.3 MPa osmotic potential, then dropped sharply at -0.5 and -0.8 MPa. Similar behavior was found in TSN(Total Soluble Nitrogen), whereas protein-N changed only slightly and proline was accumulated substantially by decreasing the osmotic potential of the seed media. Although GA, application had no any promotive influence on seed germination under water stress, it caused considerable changes in carbohydrate fractions in the growing seedlings. For example, monosaccharides were reduced, sucrose was accumulated and polysaccharides were degraded in hormone treatment as compared to water treatment. Nitrogen constituents, on the other hand, showed no clear responses to GAi treatment. The possible metabolic roles of all these fractions in the growing seedlings due to water stress were discussed.
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Claims 14
Claim · model-written summary
Germination percentage fell as osmotic potential decreased, reaching its lowest level at -0.8 MPa.
- Treatment
- decrease in the osmotic potential of seed mediae0
- Outcome
- germination percentagee0
- Direction
- reducede0
- Value
- -0.8 MPae0
Not reportedTaxonComparator
Verbatim
e0“The germination percentage was reduced with the decrease in the osmotic potential of seed media, reaching its lower value at -0.8 MPa.”
Claim · model-written summary
Water stress caused considerable sucrose accumulation.
Not reportedTaxonComparator
Verbatim
e0“Sucrose was accumulated considerably which could have had a major contribution in the total carbohydrate, while monosaccharides showed a substantial reduction and polysaccharides demonstrated no consistent pattern of changes due to water stress.”
Claim · model-written summary
Water stress substantially reduced monosaccharides.
Not reportedTaxonComparator
Verbatim
e0“monosaccharides showed a substantial reduction and polysaccharides demonstrated no consistent pattern of changes due to water stress.”
Claim · model-written summary
Polysaccharides showed no consistent change under water stress.
Not reportedTaxonComparator
Verbatim
e0“polysaccharides demonstrated no consistent pattern of changes due to water stress.”
Claim · model-written summary
Total nitrogen increased at -0.1 and -0.3 MPa.
Not reportedTaxonComparatorOutcome
Verbatim
e0“it increased considerably in -0.1 and -0.3 MPa osmotic potential”
Claim · model-written summary
Total nitrogen dropped at -0.5 and -0.8 MPa.
Not reportedTaxonComparatorOutcome
Verbatim
e0“then dropped sharply at -0.5 and -0.8 MPa.”
Claim · model-written summary
Total soluble nitrogen behaved similarly to total nitrogen.
Not reportedTaxon
Verbatim
e0“Similar behavior was found in TSN(Total Soluble Nitrogen)”
Claim · model-written summary
Protein nitrogen changed only slightly as osmotic potential declined.
- Treatment
- decreasing the osmotic potential of the seed mediae0
- Outcome
- protein-Ne0
- Direction
- changed only slightlye0
Not reportedTaxonComparator
Verbatim
e0“protein-N changed only slightly and proline was accumulated substantially by decreasing the osmotic potential of the seed media.”
Claim · model-written summary
Proline accumulated substantially as osmotic potential declined.
Not reportedTaxonComparator
Verbatim
e0“protein-N changed only slightly and proline was accumulated substantially by decreasing the osmotic potential of the seed media.”
Claim · model-written summary
GA treatment did not promote germination under water stress.
- Treatment
- GA, applicatione0
- Outcome
- seed germinatione0
- Direction
- no any promotive influencee0
- Qualifier
- under water stresse0
Not reportedTaxonComparator
Verbatim
e0“GA, application had no any promotive influence on seed germination under water stress”
Claim · model-written summary
GA treatment reduced seedling monosaccharides relative to water treatment.
Not reportedTaxon
Verbatim
e0“monosaccharides were reduced, sucrose was accumulated and polysaccharides were degraded in hormone treatment as compared to water treatment.”
Claim · model-written summary
GA treatment accumulated seedling sucrose relative to water treatment.
Not reportedTaxon
Verbatim
e0“monosaccharides were reduced, sucrose was accumulated and polysaccharides were degraded in hormone treatment as compared to water treatment.”
Claim · model-written summary
GA treatment degraded seedling polysaccharides relative to water treatment.
Not reportedTaxon
Verbatim
e0“monosaccharides were reduced, sucrose was accumulated and polysaccharides were degraded in hormone treatment as compared to water treatment.”
Claim · model-written summary
Nitrogen constituents showed no clear response to GA treatment.
Not reportedTaxonComparator
Verbatim
e0“Nitrogen constituents, on the other hand, showed no clear responses to GAi treatment.”