Abstract
In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock, was achieved through terminal/axillary bud culture using Murashige and Skoog (MS) basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and 0.1 mg/L indole-3-butyric acid (IBA). Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival of explants and their bud break. Although, the shoots were multiplied on different concentrations of cytokinins and auxins, improved multiplication was achieved with 0.5 mg/L BA and 0.01 mg/L IBA. Of the three types of explants evaluated for shoot multiplication, shoot tips on elongation were found suitable for rooting whereas, nodal cuttings and cluster of small shoots for further multiplication. Low concentrations of �-naphthyl acetic acid (NAA) was proved to be more effective for rooting as compared to IBA and indole-3-acetic acid (IAA). To overcome profuse callus formation during rooting, activated charcoal was added. In another experiment, auxin was discontinued from the rooting medium after few days, thus, resulting in better rooting frequency (72%). Thirty plantlets of Merton I. 793 were successfully hardened after 7 wks, with around 80% survival rate.
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Claims 11
Claim · model-written summary
In vitro shoot multiplication of Merton I. 793 was achieved by terminal/axillary bud culture on MS medium with 0.5-1.0 mg/L BA and 0.1 mg/L IBA.
- Taxon
- apple
Malus domesticae1 - Material
- Merton I. 793e1
- Organ / stage
- terminal/axillary bude1
- Treatment
- basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and 0.1 mg/L indole-3-butyric acid (IBA)e1
- Outcome
- shoot multiplicatione1
- Value
- 0.5-1.0 mg/Le1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock, was achieved through terminal/axillary bud culture using Murashige and Skoog (MS) basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and 0.1 mg/L indole-3-butyric acid (IBA)”
t0“An apple rootstock”
Claim · model-written summary
Shoot multiplication improved with 0.5 mg/L BA and 0.01 mg/L IBA compared with other cytokinin and auxin concentrations.
- Taxon
- appleMalus domesticat0
- Treatment
- 0.5 mg/L BA and 0.01 mg/L IBAe2
- Outcome
- multiplicatione2
- Direction
- improvede2
- Comparator
- different concentrations of cytokinins and auxinse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“the shoots were multiplied on different concentrations of cytokinins and auxins, improved multiplication was achieved with 0.5 mg/L BA and 0.01 mg/L IBA”
t0“An apple rootstock”
Claim · model-written summary
Shoot tips on elongation were found suitable for rooting among the three explant types.
- Taxon
- appleMalus domesticat0
- Organ / stage
- shoot tips on elongatione3
- Outcome
- rootinge3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“shoot tips on elongation were found suitable for rooting”
t0“An apple rootstock”
Claim · model-written summary
Nodal cuttings and clusters of small shoots were suitable for further multiplication among the three explant types.
- Taxon
- appleMalus domesticat0
- Organ / stage
- nodal cuttings and cluster of small shootse4
- Outcome
- further multiplicatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“nodal cuttings and cluster of small shoots for further multiplication”
t0“An apple rootstock”
Claim · model-written summary
Low concentrations of NAA were more effective for rooting than IBA and IAA.
- Taxon
- appleMalus domesticat0
- Treatment
- NAAe5
- Outcome
- rootinge5
- Direction
- more effectivee5
- Comparator
- IBA and indole-3-acetic acid (IAA)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“(NAA) was proved to be more effective for rooting as compared to IBA and indole-3-acetic acid (IAA)”
t0“An apple rootstock”
Claim · model-written summary
Discontinuing auxin from the rooting medium after a few days gave better rooting frequency (72%).
- Taxon
- appleMalus domesticat0
- Treatment
- auxin was discontinued from the rooting medium after few dayse6
- Outcome
- rooting frequencye6
- Direction
- bettere6
- Value
- 72%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“auxin was discontinued from the rooting medium after few days, thus, resulting in better rooting frequency (72%)”
t0“An apple rootstock”
Claim · model-written summary
Plantlets of Merton I. 793 were successfully hardened after 7 weeks.
- Taxon
- appleMalus domesticat0
- Material
- Merton I. 793e7
- Outcome
- hardenede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Thirty plantlets of Merton I. 793 were successfully hardened after 7 wks”
t0“An apple rootstock”
Claim · model-written summary
About 80% survival rate was reached by hardened plantlets of Merton I. 793.
- Taxon
- appleMalus domesticat0
- Material
- Merton I. 793e7
- Outcome
- survival ratee7
- Value
- around 80%e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Thirty plantlets of Merton I. 793 were successfully hardened after 7 wks, with around 80% survival rate”
t0“An apple rootstock”
Claim · model-written summary
Time of collection of explants influenced explant survival and bud break.
- Taxon
- appleMalus domesticat0
- Treatment
- Time of collection of explantse0
- Outcome
- survival of explants and their bud breake0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival of explants and their bud break”
t0“An apple rootstock”
Claim · model-written summary
Duration of surface sterilents influenced explant survival and bud break.
- Taxon
- appleMalus domesticat0
- Treatment
- duration of surface sterilentse0
- Outcome
- survival of explants and their bud breake0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival of explants and their bud break”
t0“An apple rootstock”
Claim · model-written summary
Growth regulators added in the medium influenced explant survival and bud break.
- Taxon
- appleMalus domesticat0
- Treatment
- growth regulators added in the mediume0
- Outcome
- survival of explants and their bud breake0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Time of collection of explants, duration of surface sterilents and growth regulators added in the medium influenced the survival of explants and their bud break”
t0“An apple rootstock”
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