Abstract
Glyoxalase I (GLYI) is a ubiquitous enzyme in all organisms that catalyzes the conversion of the potent cytotoxin methylglyoxal to S-D-lactoylglutathione. Although many reports suggest the importance of GLYI in the plant response to stress, its function in seeds requires further study. Here, we identified a heat-induced GLYI from Brassica napus seeds, BnGLYI, using a comparative proteomics approach. Two-dimensional gel analyses revealed that BnGLYI protein expression upon heat treatment was significantly elevated in thermotolerant seeds but was diminished in heat-sensitive seeds. The BnGLYI-2 and BnGLYI-3 genes from the heat-sensitive and thermotolerant cultivars, respectively, were characterized, and analyzed. Only two amino acid residue variations were found between the amino acid sequences of the two genes. Moreover, overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2. In addition, BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment, whereas these activities were diminished for BnGLYI-2 transformants. Taken together, these results indicate that overexpression of the BnGLYI-3 gene imparts thermotolerance and cold tolerance in yeast and that the variations in BnGLYI-3 may play an important role in the responses to temperature stresses.
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Claims 9
Claim · model-written summary
A heat-induced GLYI, BnGLYI, was identified from Brassica napus seeds.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Organ / stage
- seedse0
- Gene
- BnGLYIe0
- Outcome
- heat-induced GLYIe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“we identified a heat-induced GLYI from Brassica napus seeds, BnGLYI”
Claim · model-written summary
Heat treatment significantly elevated BnGLYI protein expression in thermotolerant seeds.
- Taxon
- Brassica napusBrassica napust0
- Material
- thermotolerant seedse0
- Organ / stage
- seedse0
- Treatment
- heat treatmente0
- Gene
- BnGLYIe0
- Outcome
- BnGLYI protein expressione0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnGLYI protein expression upon heat treatment was significantly elevated in thermotolerant seeds”
t0“Brassica napus seeds”
Claim · model-written summary
Heat treatment diminished BnGLYI protein expression in heat-sensitive seeds.
- Taxon
- Brassica napusBrassica napust0
- Material
- heat-sensitive seedse0
- Treatment
- heat treatmente2
- Gene
- BnGLYIe1
- Outcome
- BnGLYI protein expressione1
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was diminished in heat-sensitive seeds”
t0“Brassica napus seeds”
e1“BnGLYI protein expression upon heat treatment”
e2“heat treatment”
Claim · model-written summary
BnGLYI-2 and BnGLYI-3 differed by only two amino acid residues.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnGLYI-2 and BnGLYI-3e1
- Outcome
- amino acid residue variationse0
- Value
- two amino acid residue variationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Only two amino acid residue variations were found between the amino acid sequences of the two genes”
t0“Brassica napus seeds”
e1“The BnGLYI-2 and BnGLYI-3 genes”
Claim · model-written summary
Yeast expressing BnGLYI-3 had greater heat tolerance than yeast expressing BnGLYI-2.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressing BnGLYI-3e0
- Gene
- BnGLYI-3e0
- Outcome
- tolerance to heate0
- Direction
- enhancede0
- Qualifier
- yeast cellse0
- Comparator
- overexpressing BnGLYI-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2”
t0“Brassica napus seeds”
Claim · model-written summary
Yeast expressing BnGLYI-3 had greater cold tolerance than yeast expressing BnGLYI-2.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressing BnGLYI-3e0
- Gene
- BnGLYI-3e0
- Outcome
- cold stresse0
- Direction
- enhancede0
- Qualifier
- yeast cellse0
- Comparator
- overexpressing BnGLYI-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2”
t0“Brassica napus seeds”
Claim · model-written summary
Yeast expressing BnGLYI-3 had significantly greater GLYI activity than BnGLYI-2 transformants.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- overexpressing BnGLYI-3e1
- Gene
- BnGLYI-3e1
- Outcome
- GLYI activitye0
- Direction
- increasede0
- Comparator
- overexpressing BnGLYI-2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significantly increased GLYI activity compared to overexpressing BnGLYI-2”
t0“Brassica napus seeds”
e1“overexpressing BnGLYI-3 in yeast cells”
Claim · model-written summary
BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnGLYI-3e0
- Outcome
- superoxide dismutase activitiese0
- Direction
- enhancede0
- Qualifier
- under heat and cold treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment”
t0“Brassica napus seeds”
Claim · model-written summary
BnGLYI-2 transformants showed diminished superoxide dismutase activities.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnGLYI-2e0
- Outcome
- superoxide dismutase activitiese1
- Direction
- diminishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these activities were diminished for BnGLYI-2 transformants”
t0“Brassica napus seeds”
e1“superoxide dismutase activities”
The same questions in other papers
heat · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.