Abstract
Rapeseed (Brassica napus L.) is not only one of the most important oil crops in the world, but it is also an important vegetable crop with a high value nutrients and metabolites. However, rapeseed is often severely damaged by adverse stresses, such as low temperature, pathogen infection and so on. Glyoxalase I (GLYI) and glyoxalase II (GLYII) are two enzymes responsible for the detoxification of a cytotoxic metabolite methylglyoxal (MG) into the nontoxic S-D-lactoylglutathione, which plays crucial roles in stress tolerance in plants. Considering the important roles of glyoxalases, the GLY gene families have been analyzed in higher plans, such as rice, soybean and Chinese cabbage; however, little is known about the presence, distribution, localizations and expression of glyoxalase genes in rapeseed, a young allotetraploid. In this study, a total of 35 BnaGLYI and 30 BnaGLYII genes were identified in the B. napus genome and were clustered into six and eight subfamilies, respectively. The classification, chromosomal distribution, gene structure and conserved motif were identified or predicted. BnaGLYI and BnaGLYII proteins were mainly localized in chloroplast and cytoplasm. By using publicly available RNA-seq data and a quantitative real-time PCR analysis (qRT-PCR), the expression profiling of these genes of different tissues was demonstrated in different developmental stages as well as under stresses. The results indicated that their expression profiles varied among different tissues. Some members are highly expressed in specific tissues, BnaGLYI11 and BnaGLYI27 expressed in flowers and germinating seed. At the same time, the two genes were significantly up-regulated under heat, cold and freezing stresses. Notably, a number of BnaGLY genes showed responses to Plasmodiophora brassicae infection. Overexpression of BnGLYI11 gene in Arabidopsis thaliana seedlings confirmed that this gene conferred freezing tolerance. This study provides insight of the BnaGLYI and BnaGLYII gene families in allotetraploid B. napus and their roles in stress resistance, and important information and gene resources for developing stress resistant vegetable and rapeseed oil.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
A total of 35 BnaGLYI genes were identified in the B. napus genome, with BnaGLYI and BnaGLYII genes clustered into six and eight subfamilies, respectively.
- Taxon
- B. napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Gene
- BnaGLYIe0
- Outcome
- genes were identifiede0
- Value
- 35e0
- Qualifier
- six and eight subfamilies, respectivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“a total of 35 BnaGLYI and 30 BnaGLYII genes were identified in the B. napus genome and were clustered into six and eight subfamilies, respectively”
Claim · model-written summary
A total of 30 BnaGLYII genes were identified in the B. napus genome.
- Taxon
- B. napusBrassica napuse0
- Gene
- BnaGLYIIe0
- Outcome
- genes were identifiede0
- Value
- 30e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 35 BnaGLYI and 30 BnaGLYII genes were identified in the B. napus genome and were clustered into six and eight subfamilies, respectively”
Claim · model-written summary
BnaGLYI and BnaGLYII proteins were mainly localized in chloroplast and cytoplasm.
- Taxon
- B. napusBrassica napuse1
- Gene
- BnaGLYI and BnaGLYII proteinse0
- Outcome
- mainly localized in chloroplast and cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnaGLYI and BnaGLYII proteins were mainly localized in chloroplast and cytoplasm”
e1“B. napus genome”
Claim · model-written summary
Expression profiles of the glyoxalase genes varied among different tissues.
- Taxon
- B. napusBrassica napuse1
- Organ / stage
- different tissuese0
- Outcome
- expression profilese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their expression profiles varied among different tissues”
e1“B. napus genome”
Claim · model-written summary
BnaGLYI11 and BnaGLYI27 were expressed in flowers and germinating seed.
- Taxon
- B. napusBrassica napuse1
- Organ / stage
- flowers and germinating seede0
- Gene
- BnaGLYI11 and BnaGLYI27e0
- Outcome
- expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnaGLYI11 and BnaGLYI27 expressed in flowers and germinating seed”
e1“B. napus genome”
Claim · model-written summary
BnaGLYI11 and BnaGLYI27 were significantly up-regulated under heat, cold and freezing stresses.
- Taxon
- B. napusBrassica napuse2
- Treatment
- heat, cold and freezing stressese0
- Gene
- BnaGLYI11 and BnaGLYI27e1
- Outcome
- up-regulatede0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two genes were significantly up-regulated under heat, cold and freezing stresses”
e1“BnaGLYI11 and BnaGLYI27 expressed in flowers and germinating seed”
e2“B. napus genome”
Claim · model-written summary
A number of BnaGLY genes showed responses to Plasmodiophora brassicae infection.
- Taxon
- B. napusBrassica napuse1
- Treatment
- Plasmodiophora brassicae infectione0
- Interactor
- Plasmodiophora brassicae
.jpg?width=60)
Plasmodiophora brassicaee0pathogen - Gene
- BnaGLY genese0
- Outcome
- responsese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“a number of BnaGLY genes showed responses to Plasmodiophora brassicae infection”
e1“B. napus genome”
Claim · model-written summary
Overexpression of BnGLYI11 in Arabidopsis thaliana seedlings conferred freezing tolerance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- seedlingse0
- Treatment
- Overexpression of BnGLYI11 genee0
- Gene
- BnGLYI11e0
- Outcome
- freezing tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of BnGLYI11 gene in Arabidopsis thaliana seedlings confirmed that this gene conferred freezing tolerance”
The same questions in other papers
heat · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
- no effect
4 papers more in this group
See all 8 papers →cold · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
12 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- no effect
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.