Abstract
Increasing nitrogen supply can increase Fe and Zn concentrations in wheat grain, but the underlying mechanisms remain unclear. Size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry was used to determine Fe and Zn speciation in the soluble extracts of grain pearling fractions of two wheat cultivars grown at two N rates (100 and 350 kg of N ha(-1)). Increasing N supply increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer and decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn. Within the soluble fraction, Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment, whereas Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected. The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins.
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Claims 7
Claim · model-written summary
Increasing N supply increased the concentrations of total Fe and Zn in wheat grain.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- Increasing N supplye1
- Outcome
- concentrations of total Fe and Zne2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Increasing N supply”
e2“increased the concentrations of total Fe and Zn”
Claim · model-written summary
Increasing N supply increased the portions of Fe and Zn unextractable with a Tris-HCl buffer.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Increasing N supplye1
- Outcome
- portions of Fe and Zn unextractable with a Tris-HCl buffere2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Increasing N supply”
e2“increased the concentrations of total Fe and Zn and the portions of Fe and Zn unextractable with a Tris-HCl buffer”
Claim · model-written summary
Increasing N supply decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Increasing N supplye1
- Outcome
- concentrations of Tris-HCl-extractable (soluble) Fe and Zne2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Increasing N supply”
e2“decreased the concentrations of Tris-HCl-extractable (soluble) Fe and Zn”
Claim · model-written summary
Soluble Fe bound to low molecular weight compounds was decreased by 5-12% by the high N treatment.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- the high N treatmente1
- Compound
- Fee1
- Outcome
- Fe and Zn bound to low molecular weight compoundse1
- Direction
- decreasede1
- Value
- 5-12%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment”
Claim · model-written summary
Soluble Zn bound to low molecular weight compounds was decreased by 4-37% by the high N treatment.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- the high N treatmente1
- Compound
- Zne1
- Outcome
- Fe and Zn bound to low molecular weight compoundse1
- Direction
- decreasede1
- Value
- 4-37%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Fe and Zn bound to low molecular weight compounds, likely to be Fe-nicotianamine and Fe-deoxymugineic acid or Zn-nicotianamine, were decreased by 5-12% and 4-37%, respectively, by the high N treatment”
Claim · model-written summary
Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected by the high N treatment.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- the high N treatmente2
- Outcome
- Fe and Zn bound to soluble high molecular weight or soluble phytate fractionse1
- Direction
- less affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“Fe and Zn bound to soluble high molecular weight or soluble phytate fractions were less affected”
e2“the high N treatment”
Claim · model-written summary
The authors attribute the positive effect of N on grain Fe and Zn concentrations to an increased sink in the grain, probably water-insoluble proteins.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Ne1
- Outcome
- grain Fe and Zn concentrationse1
- Qualifier
- increased sink in the grain, probably in the form of water-insoluble proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat”
e1“The positive effect of N on grain Fe and Zn concentrations was attributed to an increased sink in the grain, probably in the form of water-insoluble proteins”
The same questions in other papers
nitrogen · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
51 papers in the same direction as this paper · 114 in the opposite direction
- positive
- positive
- positive
162 papers more in this group
See all 166 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.