Abstract
Jumonji C (JmjC) domain-containing protein 14 (JMJ14) is an H3K4-specific histone demethylase that has important roles in RNA-mediated gene silencing and flowering time regulation in Arabidopsis . However, how JMJ14 is recruited to its target genes remains unclear. Here, we show that the C-terminal FYRN (F/Y-rich N terminus) and FYRC (F/Y-rich C terminus) domains of JMJ14 are required for RNA silencing and flowering time regulation. Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains, and H3K4me3 is increased. FYRN and FYRC domains interact with a pair of NAC (NAM, ATAF, CUC) domain-containing transcription factors, NAC050 and NAC052. Genome-wide chromatin immunoprecipitation analysis revealed that JMJ14 and NAC050/052 share a set of common target genes with CTTGNNNNNCAAG consensus sequences. Mutations in either NAC052 or NAC050 impair RNA-mediated gene silencing. Together, our findings demonstrate an important role of FYRN and FYRC domains in targeting JMJ14 through direct interaction with NAC050/052 proteins, which reveals a novel mechanism of histone demethylase recruitment.
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Claims 8
Claim · model-written summary
JMJ14 FYRN and FYRC domains were required for RNA silencing and flowering time regulation.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- JMJ14e0
- Outcome
- RNA silencing and flowering time regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the C-terminal FYRN (F/Y-rich N terminus) and FYRC (F/Y-rich C terminus) domains of JMJ14 are required for RNA silencing and flowering time regulation”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
Deleting the FYRN and FYRC domains abolished JMJ14 chromatin binding.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- deletion of its FYRN and FYRC domainse0
- Gene
- JMJ14e0
- Outcome
- Chromatin binding of JMJ14e0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
Deleting the FYRN and FYRC domains increased H3K4me3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- deletion of its FYRN and FYRC domainse0
- Outcome
- H3K4me3e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromatin binding of JMJ14 is lost upon deletion of its FYRN and FYRC domains, and H3K4me3 is increased”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
FYRN and FYRC domains interacted with NAC050 and NAC052.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- NAC050 and NAC052e0
- Outcome
- interacte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FYRN and FYRC domains interact with a pair of NAC (NAM, ATAF, CUC) domain-containing transcription factors, NAC050 and NAC052”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
JMJ14 and NAC050/052 shared target genes with CTTGNNNNNCAAG consensus sequences.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- JMJ14 and NAC050/052e0
- Outcome
- common target genes with CTTGNNNNNCAAG consensus sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JMJ14 and NAC050/052 share a set of common target genes with CTTGNNNNNCAAG consensus sequences”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
Mutation of NAC052 impaired RNA-mediated gene silencing.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in either NAC052 or NAC050e0
- Gene
- NAC052e0
- Outcome
- RNA-mediated gene silencinge0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in either NAC052 or NAC050 impair RNA-mediated gene silencing”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
Mutation of NAC050 impaired RNA-mediated gene silencing.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutations in either NAC052 or NAC050e0
- Gene
- NAC050e0
- Outcome
- RNA-mediated gene silencinge0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in either NAC052 or NAC050 impair RNA-mediated gene silencing”
t0“flowering time regulation in Arabidopsis”
Claim · model-written summary
Direct interaction with NAC050/052 targets JMJ14 through its FYRN and FYRC domains.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- JMJ14e0
- Outcome
- targeting JMJ14e0
- Qualifier
- direct interaction with NAC050/052 proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FYRN and FYRC domains in targeting JMJ14 through direct interaction with NAC050/052 proteins”
t0“flowering time regulation in Arabidopsis”
The same questions in other papers
loss of function · molecular: expression · ArabidopsisPositive and negative results
158 papers in the same direction as this paper · 38 in the opposite direction
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