Abstract
SummaryRegeneration of shoots from in-vitro grown leaf tissues of the pear cv. Conference was achieved at rates of up to 40%. The highest regeneration percentage was achieved using a phytohormone combination of benzylamino purine (5 mg 1−1) and 1-naphthalene acetic acid (1.0 mg 1−1) on a basal medium of Murashige and Skoog, to which had been added 200 mg I−1 of the antibiotic cefotaxime. The percentage regeneration was reduced when a higher concentration of cefotaxime (400 mg 1−1) was used. Cefotaxime had little beneficial effect on regeneration at a lower concentration of 1-naphthalene acetic acid (0.5 mg l−1). Regenerated shoots were easily micropropagated, rooted and transplanted to soil. To date, the plants have a true-to-type phenotypic appearance. Regeneration was also achieved with other cultivars—‘Abbé Fetel’, ‘Doyenne d’Hiver’, ‘Doyenne du Comice’ and ‘Passé Crassane’—but percentage regeneration was lower and, apart from ‘Passé Crassane’, the regenerated shoots were weak and could not be subcultured.
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Claims 7
Claim · model-written summary
Shoot regeneration from in-vitro leaves of pear cv. Conference reached up to 40%.
- Taxon
- Pyrus communis
Pyrus communist0 - Material
- Conferencee1
- Organ / stage
- in-vitro grown leaf tissuese1
- Outcome
- Regeneration of shootse1
- Value
- up to 40%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e1“Regeneration of shoots from in-vitro grown leaf tissues of the pear cv. Conference was achieved at rates of up to 40%”
Claim · model-written summary
The highest regeneration percentage was obtained with benzylamino purine (5 mg 1−1) and 1-naphthalene acetic acid (1.0 mg 1−1).
- Taxon
- Pyrus communis
Pyrus communist0 - Treatment
- a phytohormone combination of benzylamino purine (5 mg 1−1) and 1-naphthalene acetic acid (1.0 mg 1−1)e2
- Outcome
- regeneration percentagee2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e2“The highest regeneration percentage was achieved using a phytohormone combination of benzylamino purine (5 mg 1−1) and 1-naphthalene acetic acid (1.0 mg 1−1)”
Claim · model-written summary
A higher cefotaxime concentration (400 mg 1−1) reduced the percentage regeneration compared with 200 mg I−1.
- Taxon
- Pyrus communis
Pyrus communist0 - Treatment
- a higher concentration of cefotaxime (400 mg 1−1)e3
- Outcome
- percentage regeneratione3
- Direction
- reducede3
- Comparator
- 200 mg I−1 of the antibiotic cefotaximee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e3“The percentage regeneration was reduced when a higher concentration of cefotaxime (400 mg 1−1) was used”
e4“200 mg I−1 of the antibiotic cefotaxime”
Claim · model-written summary
Cefotaxime had little beneficial effect on regeneration at a lower naphthalene acetic acid concentration (0.5 mg l−1).
- Taxon
- Pyrus communis
Pyrus communist0 - Outcome
- little beneficial effect on regeneratione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e5“Cefotaxime had little beneficial effect on regeneration at a lower concentration of 1-naphthalene acetic acid (0.5 mg l−1)”
Claim · model-written summary
Regenerated shoots were easily micropropagated, rooted and transplanted to soil.
- Taxon
- Pyrus communis
Pyrus communist0 - Organ / stage
- Regenerated shootse6
- Outcome
- micropropagated, rooted and transplanted to soile6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e6“Regenerated shoots were easily micropropagated, rooted and transplanted to soil”
Claim · model-written summary
Regenerated pear plants have a true-to-type phenotypic appearance.
- Taxon
- Pyrus communis
Pyrus communist0 - Outcome
- true-to-type phenotypic appearancee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e7“To date, the plants have a true-to-type phenotypic appearance”
Claim · model-written summary
Regeneration percentage in other pear cultivars was lower than in Conference.
- Taxon
- Pyrus communis
Pyrus communist0 - Material
- other cultivarse5
- Outcome
- percentage regeneratione6
- Direction
- lowere6
- Comparator
- Conferencee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
t0“other pear cultivars (Pyrus communisL.)”
e5“Regeneration was also achieved with other cultivars”
e6“but percentage regeneration was lower”
e1“Regeneration of shoots from in-vitro grown leaf tissues of the pear cv. Conference was achieved at rates of up to 40%”
The same questions in other papers
1-naphthaleneacetic acid · growth · Pyrus communis (pear)Positive and negative results
5 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
3 papers more in this group
See all 7 papers →cefotaxime · growth · Pyrus communis (pear)Results agree
1 paper in the same direction as this paper
- no effect
class · growth · Pyrus communis (pear)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.