Abstract
Efficient direct shoot regeneration of two pear (Pyrus communis L.), namely 'bartlett' and 'dargazi' was successfully developed for the use in future genetic engineering research. The basal MS and NN media supplemented with different concentrations of TDZ (0, 2.5, 5, 7.5 ?M) or BAP (0, 4, 8, 16 ?M) in combination with NAA (1 ?M) were compared. The result showed that direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media. 'dargazi' had higher rate of regeneration compared to 'bartlett' and in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves. Although the highest per cent of regeneration in 'bartlett' (38) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, but the differences in shoot regeneration between media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest per cent of regeneration in 'dargazi' (56) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA. Plant Tissue Cult. & Biotech. 24(1): 87-92, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19215
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Claims 6
Claim · model-written summary
Pear adventitious shoot regeneration depended strongly on genotype, explant type and culture medium.
- Taxon
- pear
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise0 - Material
- genotypee0
- Organ / stage
- explant typee0
- Treatment
- culture mediae0
- Outcome
- direct adventitious shoot regeneratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media”
Claim · model-written summary
'dargazi' regenerated at a higher rate than 'bartlett'.
- Taxon
- PearPyrus communist0
- Material
- 'dargazi'e0
- Outcome
- rate of regeneratione0
- Direction
- highere0
- Comparator
- 'bartlett'e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“'dargazi' had higher rate of regeneration compared to 'bartlett'”
t0“Pear (Pyrus communis L.)”
Claim · model-written summary
Both pear cultivars had their highest regeneration percentage in lower leaf sections.
- Taxon
- PearPyrus communist0
- Organ / stage
- lower sections of the leavese0
- Outcome
- per cent of regeneratione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves”
t0“Pear (Pyrus communis L.)”
Claim · model-written summary
For 'bartlett', the highest regeneration percentage was 38 in NN medium with 2.5 µM TDZ and 1 µM NAA.
- Taxon
- PearPyrus communist0
- Material
- 'bartlett'e0
- Treatment
- NN medium containing 2.5 µM TDZ and 1 µM NAAe0
- Outcome
- per cent of regeneratione0
- Direction
- higheste0
- Value
- 38e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest per cent of regeneration in 'bartlett' (38) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA”
t0“Pear (Pyrus communis L.)”
Claim · model-written summary
For 'bartlett', shoot regeneration did not differ significantly between media containing 5 or 7.5 µM TDZ with 1 µM NAA.
- Taxon
- PearPyrus communist0
- Material
- 'bartlett'e1
- Treatment
- 5 or 7.5 µM TDZ and 1 µM NAAe0
- Outcome
- shoot regeneratione0
- Comparator
- mediae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“differences in shoot regeneration between media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant”
t0“Pear (Pyrus communis L.)”
e1“regeneration in 'bartlett' (38)”
Claim · model-written summary
For 'dargazi', the highest regeneration percentage was 56 in NN medium with 7.5 µm TDZ and 1 µm NAA.
- Taxon
- PearPyrus communist0
- Material
- 'dargazi'e0
- Treatment
- NN medium containing 7.5 µm TDZ and 1 µm NAAe0
- Outcome
- per cent of regeneratione0
- Direction
- higheste0
- Value
- 56e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest per cent of regeneration in 'dargazi' (56) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA”
t0“Pear (Pyrus communis L.)”
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