Abstract
By using the protoplast fusion techniqlle we obtained rice-soybean hybrid calli having soybean nuclei and chloroplasts of both parents. In the brown hybrid calli sorting-out of chloroplasts occurred and green calli having soybean chloroplasts and white calli having rice chloroplasts were segregated (NIIZEKI et al. 1985). The heteroplasmic hybrid calli with both parent chloroplasts were extremely sensitive to streptomycin under both light and dark conditions, while the parent calli and the green and white calli produced by the sorting-out of chloroplasts were resistant to streptomycin under light conditions, but very sensitive under dark conditions. From this result it can be assumed that the responses of these calli to streptomycin may be related to the chloroplast for the follow-ing reasons : Firstly, the difference in the response of the parent calli or the calli with sorted-out chloroplasts to streptomycin under light and dark conditions suggests that the sensitivity to streptomycin may be related to the chloroplasts which require light for their functions and secondly, the recovery of streptomycin resistance in the calli with sorted-out chloroplasts indicates that the chloroplasts respond to streptomycin. However, the pcssibility that the sensitivity to streptomycin resides in the other organelles could not be excluded. Also, the reason for the heteroplasmic state of the calli being very sensitive even under light conditions remains unknown. After long term culture streptomycin resistant portions in the heteroplasmic hybrid calli increased in number under light conditions. This means that the sorting-out of chloroplasts progressed in the hybrid calli and the number of callus portions or cells with only one of the parent chloroplasts increased.
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Claims 6
Claim · model-written summary
The fusion produced hybrid calli with soybean nuclei and chloroplasts from both parents.
Verbatim
e0“we obtained rice-soybean hybrid calli having soybean nuclei and chloroplasts of both parents”
Claim · model-written summary
Chloroplast sorting segregated green calli with soybean chloroplasts and white calli with rice chloroplasts.
- Taxon
- soybean
Glycine maxe0 - Outcome
- green calli having soybean chloroplasts and white calli having rice chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“green calli having soybean chloroplasts and white calli having rice chloroplasts were segregated”
Claim · model-written summary
Heteroplasmic hybrid calli were extremely sensitive to streptomycin in light and darkness.
- Taxon
- Rice and SoybeanGlycine max
Oryza sativat0 - Treatment
- streptomycine0
- Outcome
- heteroplasmic hybrid calli with both parent chloroplasts were extremely sensitivee0
- Direction
- extremely sensitivee0
- Qualifier
- under both light and dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heteroplasmic hybrid calli with both parent chloroplasts were extremely sensitive to streptomycin under both light and dark conditions”
t0“Rice and Soybean”
Claim · model-written summary
Parent and sorted calli resisted streptomycin in light.
- Taxon
- Rice and SoybeanGlycine maxOryza sativat0
- Treatment
- streptomycine0
- Outcome
- parent calli and the green and white calli produced by the sorting-out of chloroplasts were resistante0
- Direction
- resistante0
- Qualifier
- under light conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the parent calli and the green and white calli produced by the sorting-out of chloroplasts were resistant to streptomycin under light conditions”
t0“Rice and Soybean”
Claim · model-written summary
Parent and sorted calli were very sensitive to streptomycin in darkness.
- Taxon
- Rice and SoybeanGlycine maxOryza sativat0
- Treatment
- streptomycine0
- Outcome
- very sensitivee0
- Direction
- very sensitivee0
- Qualifier
- under dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the parent calli and the green and white calli produced by the sorting-out of chloroplasts were resistant to streptomycin under light conditions, but very sensitive under dark conditions”
t0“Rice and Soybean”
Claim · model-written summary
Streptomycin resistant portions of heteroplasmic hybrid calli increased after long term culture in light.
- Taxon
- Rice and SoybeanGlycine maxOryza sativat0
- Outcome
- streptomycin resistant portions in the heteroplasmic hybrid callie0
- Direction
- increasede0
- Qualifier
- under light conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After long term culture streptomycin resistant portions in the heteroplasmic hybrid calli increased in number under light conditions.”
t0“Rice and Soybean”
The same questions in other papers
streptomycin · disease · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.