Abstract
cDNA for an acid invertase (EC 3.2.1.26) of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants under the control of the cauliflower mosaic virus 35S promoter in the antisense orientation. The antisense gene effectively suppressed the invertase activity in soluble and cell wall fractions from ripening fruits. The sucrose content of fruits of the transformants was markedly increased, while the hexose content was reduced. These results indicate that acid invertase is one of main determinants of the sugar composition of tomato fruit. The invertase activity in the cell wall fraction of the leaf tissues of the transformants was not suppressed to the same extent as that in the soluble fraction. Wounding of the control leaf tissues induced invertase activity in both soluble and cell wall fractions. The induction of activity in the soluble fraction was suppressed by the antisense gene, while that in the cell wall fraction was unaffected. These findings suggest that mRNA for some other invertase, in particular, the mRNA for a cell wall-bound invertase, was present in leaves.
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Claims 9
Claim · model-written summary
Antisense expression suppressed invertase activity in soluble and cell wall fractions of ripening tomato fruit.
- Taxon
- tomato
Solanum lycopersicume1 - Organ / stage
- ripening fruitse0
- Treatment
- antisense genee0
- Gene
- acid invertasee1
- Outcome
- invertase activity in soluble and cell wall fractionse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antisense gene effectively suppressed the invertase activity in soluble and cell wall fractions from ripening fruits.”
e1“cDNA for an acid invertase (EC 3.2.1.26) of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants under the control of the cauliflower mosaic virus 35S promoter in the antisense orientation.”
Claim · model-written summary
Fruit sucrose content markedly increased in antisense transformants.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- transformantse0
- Outcome
- sucrose content of fruitse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The sucrose content of fruits of the transformants was markedly increased”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
Fruit hexose content was reduced in antisense transformants.
- Taxon
- tomatoSolanum lycopersicume2
- Treatment
- transformantse1
- Outcome
- hexose contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the hexose content was reduced”
e1“of the transformants was markedly increased, while the hexose content was reduced”
e2“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
Acid invertase was interpreted as a main determinant of tomato fruit sugar composition.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- acid invertasee0
- Outcome
- sugar composition of tomato fruite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“acid invertase is one of main determinants of the sugar composition of tomato fruit”
Claim · model-written summary
Antisense suppression of cell wall invertase activity in leaves was weaker than suppression in the soluble fraction.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- leaf tissuese0
- Treatment
- transformantse0
- Outcome
- invertase activity in the cell wall fractione0
- Direction
- not suppressed to the same extente0
- Comparator
- soluble fractione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The invertase activity in the cell wall fraction of the leaf tissues of the transformants was not suppressed to the same extent as that in the soluble fraction.”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
Wounding induced soluble and cell wall invertase activity in control tomato leaves.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- leaf tissuese0
- Treatment
- Woundinge0
- Outcome
- invertase activity in both soluble and cell wall fractionse0
- Direction
- inducede0
- Comparator
- control leaf tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding of the control leaf tissues induced invertase activity in both soluble and cell wall fractions.”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
The antisense gene suppressed wound induction of invertase activity in the leaf soluble fraction.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- antisense genee0
- Outcome
- induction of activity in the soluble fractione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of activity in the soluble fraction was suppressed by the antisense gene”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
The antisense gene left wound induced activity in the leaf cell wall fraction unaffected.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- antisense genee0
- Outcome
- that in the cell wall fractione0
- Direction
- unaffectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of activity in the soluble fraction was suppressed by the antisense gene, while that in the cell wall fraction was unaffected.”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
Claim · model-written summary
The authors inferred that leaves contain mRNA for another invertase, particularly a cell wall bound invertase.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- leavese0
- Gene
- cell wall-bound invertasee0
- Outcome
- mRNA for some other invertasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA for some other invertase, in particular, the mRNA for a cell wall-bound invertase, was present in leaves.”
e1“of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.