Abstract
Hydration at low temperatures, commonly referred to as cold stratification, is widely used for releasing dormancy and triggering germination in a wide range of species including wheat. However, the molecular mechanism that underlies its effect on germination has largely remained unknown. Our previous studies showed that methyl-jasmonate, a derivative of jasmonic acid (JA), promotes dormancy release in wheat. In this study, we found that cold-stimulated germination of dormant grains correlated with a transient increase in JA content and expression of JA biosynthesis genes in the dormant embryos after transfer to 20 (o)C. The induction of JA production was dependent on the extent of cold imbibition and precedes germination. Blocking JA biosynthesis with acetylsalicylic acid (ASA) inhibited the cold-stimulated germination in a dose-dependent manner. In addition, we have explored the relationship between JA and abscisic acid (ABA), a well-known dormancy promoter, in cold regulation of dormancy. We found an inverse relationship between JA and ABA content in dormant wheat embryos following stratification. ABA content decreased rapidly in response to stratification, and the decrease was reversed by addition of ASA. Our results indicate that the action of JA on cold-stratified grains is mediated by suppression of two key ABA biosynthesis genes, TaNCED1 and TaNCED2.
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Claims 7
Claim · model-written summary
Cold-stimulated germination correlated with a transient increase in JA content and JA biosynthesis gene expression.
- Taxon
- wheat
Triticum aestivumt0 - Organ / stage
- dormant embryose0
- Compound
- JAe0
- Outcome
- cold-stimulated germination of dormant grainse0
- Direction
- increasee0
- Setting
- 20 (o)Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold-stimulated germination of dormant grains correlated with a transient increase in JA content and expression of JA biosynthesis genes in the dormant embryos after transfer to 20 (o)C”
t0“wheat”
Claim · model-written summary
JA production induction depended on the extent of cold imbibition and preceded germination.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- cold imbibitione0
- Outcome
- induction of JA productione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of JA production was dependent on the extent of cold imbibition and precedes germination.”
t0“wheat”
Claim · model-written summary
ASA inhibition of JA biosynthesis inhibited cold-stimulated germination in a dose-dependent manner.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- acetylsalicylic acid (ASA)e0
- Outcome
- cold-stimulated germinatione0
- Direction
- inhibitede0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Blocking JA biosynthesis with acetylsalicylic acid (ASA) inhibited the cold-stimulated germination in a dose-dependent manner.”
t0“wheat”
Claim · model-written summary
JA and ABA content were inversely related in dormant wheat embryos after stratification.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- dormant wheat embryose0
- Compound
- JAe0
- Outcome
- inverse relationship between JA and ABA contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an inverse relationship between JA and ABA content in dormant wheat embryos following stratification”
Claim · model-written summary
ABA content decreased rapidly after stratification.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- stratificatione0
- Outcome
- ABA contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA content decreased rapidly in response to stratification”
t0“wheat”
Claim · model-written summary
Adding ASA reversed the stratification-associated ABA decrease.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- addition of ASAe0
- Outcome
- ABA contente1
- Direction
- reversede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the decrease was reversed by addition of ASA”
e1“ABA content decreased rapidly in response to stratification”
t0“wheat”
Claim · model-written summary
The authors infer that JA acts on cold-stratified grains by suppressing TaNCED1 and TaNCED2.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaNCED1 and TaNCED2e0
- Compound
- JAe0
- Outcome
- action of JA on cold-stratified grainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the action of JA on cold-stratified grains is mediated by suppression of two key ABA biosynthesis genes, TaNCED1 and TaNCED2”
t0“wheat”
The same questions in other papers
acetylsalicylic acid · growth · Triticum aestivum (wheat)Positive and negative results
1 in the opposite direction
- positive
seed treatment · physiology: hormones · Triticum aestivum (wheat)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.