Abstract
Five excellent clones of Populus tomentosa were used in this study.Sterile shoots were used as explants to induce bud differentiation and growth, as well as rooting in different medium. The potential of plantlet regeneration was compared with different clones. The results showed that all five clones of P. tomentosa could obtain regeneration plantlets by organogenesis in vitro. But a marked difference was observed in axillary shoots number and rooting rate, in which No.4 clone axillary shoot number is 122.7% of the average and No. 1 only averages 78.4%. No. 2 rooting rate reachs 60% and No. 3 only 28.7%. It indicated that capacity of shoot proliferation and rooting was genetically controlled. The 0.5mg/L 6-BA concentration may be the optimal value for induction and proliferation of shoot for most clones in vitro. Although average shoot number reached 8.8, rooting percentage was only 49.5%. 1/2 MS basal medium was suitable to rooting for clones. The rooting capacity could be improved by supplementing low concentration auxin. At the same time there existed a visible media×clone interaction in plantlet regeneration capacity for P. tomentosa.
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Claims 12
Claim · model-written summary
All five clones regenerated plantlets by organogenesis in vitro.
- Taxon
- Populus tomentosa
Populus tomentosat0 - Material
- five clonese0
- Outcome
- regeneration plantlets by organogenesis in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all five clones of P. tomentosa could obtain regeneration plantlets by organogenesis in vitro”
t0“Populus tomentosa”
Claim · model-written summary
No.4 clone axillary shoot number was 122.7% of the average.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Material
- No.4 clonee0
- Outcome
- axillary shoot numbere0
- Value
- 122.7%e0
- Comparator
- the averagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No.4 clone axillary shoot number is 122.7% of the average”
t0“Populus tomentosa”
Claim · model-written summary
No. 1 clone axillary shoot number was 78.4% of the average.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Material
- No. 1e0
- Outcome
- averagese0
- Value
- 78.4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No. 1 only averages 78.4%”
t0“Populus tomentosa”
Claim · model-written summary
No. 2 clone rooting rate reached 60%.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Material
- No. 2e0
- Outcome
- rooting ratee0
- Value
- 60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No. 2 rooting rate reachs 60%”
t0“Populus tomentosa”
Claim · model-written summary
No. 3 clone rooting rate was 28.7%.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Material
- No. 3e0
- Outcome
- 28.7%e0
- Value
- 28.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No. 3 only 28.7%”
t0“Populus tomentosa”
Claim · model-written summary
Shoot proliferation and rooting capacity were genetically controlled.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Outcome
- capacity of shoot proliferation and rootinge0
- Qualifier
- genetically controllede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“capacity of shoot proliferation and rooting was genetically controlled”
t0“Populus tomentosa”
Claim · model-written summary
0.5mg/L 6-BA may be optimal for shoot induction and proliferation for most clones.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Treatment
- 0.5mg/L 6-BAe0
- Outcome
- optimal value for induction and proliferation of shoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 0.5mg/L 6-BA concentration may be the optimal value for induction and proliferation of shoot for most clones in vitro”
t0“Populus tomentosa”
Claim · model-written summary
Average shoot number reached 8.8.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Outcome
- average shoot numbere0
- Value
- 8.8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“average shoot number reached 8.8”
t0“Populus tomentosa”
Claim · model-written summary
Rooting percentage was 49.5%.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Outcome
- rooting percentagee0
- Value
- 49.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooting percentage was only 49.5%”
t0“Populus tomentosa”
Claim · model-written summary
1/2 MS basal medium was suitable for rooting.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Treatment
- 1/2 MS basal mediume0
- Outcome
- suitable to rootinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1/2 MS basal medium was suitable to rooting for clones”
t0“Populus tomentosa”
Claim · model-written summary
Low concentration auxin improved rooting capacity.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Treatment
- supplementing low concentration auxine0
- Outcome
- rooting capacitye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooting capacity could be improved by supplementing low concentration auxin”
t0“Populus tomentosa”
Claim · model-written summary
Media and clone interacted in regeneration capacity.
- Taxon
- Populus tomentosaPopulus tomentosat0
- Material
- clonee0
- Outcome
- plantlet regeneration capacitye0
- Qualifier
- media×clone interactione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there existed a visible media×clone interaction in plantlet regeneration capacity for P. tomentosa”
t0“Populus tomentosa”