Paper · 2012 · Acta Horticulturae Sinica

Determination of Sulforaphane by High Performance Liquid Chromatography and Genetic Analysis of DH Population in Broccoli Florets

Zhansheng Li; Liu Yu-mei; Zhiyuan Fang; et al.

5
claims
10
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

A doubled haploid(DH)population derived from broccoli combination of inbred lines 86101 and 90196 was used to investigate the inheritance of sulforaphane content in broccoli(Brassica oleracea L.var.italica Planch.)florets by high performance liquid chromatography(HPLC)and mixed major gene plus polygene inheritance model.The population consists of 176 DH lines was obtained by F1 microspore culture,and the trial was set by random complete block design with three replications.The optimal model was three major genes plus polygenes.The heritability of major genes and polygenes was 89.28%and 2.58%individually which showed major genes had a key role in controlling sulforaphane content.At the same time,major genes followed addictive effect and epistatic effect.

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Claims 5

Observationobservedoriginal resultmeasured

Claim · model-written summary

The optimal inheritance model for broccoli floret sulforaphane content was three major genes plus polygenes.

Organ / stage
floretse1
Outcome
three major genes plus polygenese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The optimal model was three major genes plus polygenes.”

✓ located verbatim in the source · abstract, characters 478–533 · show in text ↑
e1

“sulforaphane content in broccoli(Brassica oleracea L.var.italica Planch.)florets”

✓ located verbatim in the source · abstract, characters 141–221 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Major-gene heritability for broccoli floret sulforaphane content was 89.28%.

Outcome
heritability of major genese0
Value
89.28%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The heritability of major genes and polygenes was 89.28%and 2.58%individually”

✓ located verbatim in the source · abstract, characters 533–610 · show in text ↑
e1

“sulforaphane content in broccoli(Brassica oleracea L.var.italica Planch.)florets”

✓ located verbatim in the source · abstract, characters 141–221 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Polygene heritability for broccoli floret sulforaphane content was 2.58%.

Outcome
heritability of major genes and polygenese0
Value
2.58%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The heritability of major genes and polygenes was 89.28%and 2.58%individually”

✓ located verbatim in the source · abstract, characters 533–610 · show in text ↑
e1

“sulforaphane content in broccoli(Brassica oleracea L.var.italica Planch.)florets”

✓ located verbatim in the source · abstract, characters 141–221 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Major genes had a key role in controlling broccoli floret sulforaphane content.

Gene
major genese0
Outcome
sulforaphane contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“major genes had a key role in controlling sulforaphane content”

✓ located verbatim in the source · abstract, characters 624–686 · show in text ↑
e1

“broccoli(Brassica oleracea L.var.italica Planch.)florets”

✓ located verbatim in the source · abstract, characters 165–221 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Major genes showed additive and epistatic effects in broccoli.

Gene
major genese0
Outcome
addictive effect and epistatic effecte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. italica
Brassica oleracea var. italica · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“major genes followed addictive effect and epistatic effect”

✓ located verbatim in the source · abstract, characters 704–762 · show in text ↑
e1

“broccoli(Brassica oleracea L.var.italica Planch.)florets”

✓ located verbatim in the source · abstract, characters 165–221 · show in text ↑