Abstract
The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials.The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃.The effects of sulfite oxidase overexpression on the relative electrical conductivity,sulfite content,lyconpene and maturity diffrence in transgenic tomato were studied.The results showed that electrical conductivity and sulfite residual of Ri fruits increased,lycopene content decreased,fruit color change accelerated.Tissue rotting and fungal growth of Ri fruit appeared 4d after B.cinerea inoculation,and surrounding pericarp tissue developed.In contrast,the relative electrical conductivity and sulfite residual of OE fruits decreased,lycopene level remained normal,fruit color change delayed.The growth of fungus in OE fruits was limited to the puncture sites and soft rot symptoms did not develop after B.cinerea inoculation for 4d.The study showed that sulfite oxidase gene overexpression in fruit could decrease sulfite residual,and improve the quality and storage properties of the tomato with SO2 fumigation.
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Claims 10
Claim · model-written summary
In Ri tomato fruit fumigated with SO2, electrical conductivity and sulfite residual increased relative to wild type.
- Taxon
- tomato
Solanum lycopersicume1 - Material
- RNA interferred(Ri)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- electrical conductivity and sulfite residuale2
- Direction
- increasede2
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e2“electrical conductivity and sulfite residual of Ri fruits increased,lycopene content decreased,fruit color change accelerated”
Claim · model-written summary
In Ri tomato fruit fumigated with SO2, lycopene content decreased relative to wild type.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- RNA interferred(Ri)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- lycopene contente2
- Direction
- decreasede2
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e2“electrical conductivity and sulfite residual of Ri fruits increased,lycopene content decreased,fruit color change accelerated”
Claim · model-written summary
In Ri tomato fruit fumigated with SO2, fruit color change was accelerated relative to wild type.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- RNA interferred(Ri)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- fruit color changee2
- Direction
- acceleratede2
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e2“electrical conductivity and sulfite residual of Ri fruits increased,lycopene content decreased,fruit color change accelerated”
Claim · model-written summary
In OE tomato fruit fumigated with SO2, electrical conductivity and sulfite residual decreased relative to wild type.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- overexpressed(OE)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- electrical conductivity and sulfite residuale4
- Direction
- decreasede4
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e4“In contrast,the relative electrical conductivity and sulfite residual of OE fruits decreased,lycopene level remained normal,fruit color change delayed”
Claim · model-written summary
In OE tomato fruit fumigated with SO2, lycopene level remained normal relative to wild type.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- overexpressed(OE)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- lycopene levele4
- Direction
- remained normale4
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e4“In contrast,the relative electrical conductivity and sulfite residual of OE fruits decreased,lycopene level remained normal,fruit color change delayed”
Claim · model-written summary
In OE tomato fruit fumigated with SO2, fruit color change was delayed relative to wild type.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- overexpressed(OE)e1
- Organ / stage
- mature green unripe tomato fruitse0
- Treatment
- fumigated with SO2e0
- Outcome
- fruit color changee4
- Direction
- delayede4
- Comparator
- wild typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mature green unripe tomato fruits were fumigated with SO2,then stored at 12℃”
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e4“In contrast,the relative electrical conductivity and sulfite residual of OE fruits decreased,lycopene level remained normal,fruit color change delayed”
Claim · model-written summary
Ri tomato fruit showed tissue rotting and fungal growth 4 days after B. cinerea inoculation.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- Rie3
- Treatment
- B.cinerea inoculatione3
- Interactor
- B.cinereae3pathogen
- Outcome
- tissue rotting and fungal growthe3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e3“Tissue rotting and fungal growth of Ri fruit appeared 4d after B.cinerea inoculation”
Claim · model-written summary
In OE tomato fruit, fungal growth after B. cinerea inoculation was limited to the puncture sites.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- OEe5
- Treatment
- B.cinerea inoculatione5
- Interactor
- B.cinereae5pathogen
- Outcome
- growth of funguse5
- Qualifier
- limited to the puncture sitese5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e5“The growth of fungus in OE fruits was limited to the puncture sites and soft rot symptoms did not develop after B.cinerea inoculation for 4d”
Claim · model-written summary
In OE tomato fruit, soft rot symptoms did not develop 4 days after B. cinerea inoculation.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- OEe5
- Treatment
- B.cinerea inoculatione5
- Interactor
- B.cinereae5pathogen
- Outcome
- soft rot symptomse5
- Qualifier
- for 4de5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The sulfite oxidase gene(SO) overexpressed(OE),RNA interferred(Ri) and wild type tomato were used as materials”
e5“The growth of fungus in OE fruits was limited to the puncture sites and soft rot symptoms did not develop after B.cinerea inoculation for 4d”
Claim · model-written summary
Sulfite oxidase gene overexpression in fruit was interpreted as improving the quality and storage properties of SO2-fumigated tomato.
- Taxon
- tomatoSolanum lycopersicume6
- Treatment
- sulfite oxidase gene overexpressione6
- Outcome
- quality and storage propertiese6
- Direction
- improvee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“sulfite oxidase gene overexpression in fruit could decrease sulfite residual,and improve the quality and storage properties of the tomato with SO2 fumigation”
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