Abstract
【Objective】 The objective of this study is to investigate the roles of NSvc4 and CP proteins of Rice stripe virus(RSV) in pathogenicity to reveal the mechanisms underlying RSV pathogenesis.【Method】 The subcellular localization patterns of six RSV proteins were studied by expressing them in epidermal cells of Nicotiana benthamiana through Agrobacterium inoculation.The interaction between NSvc4 and five other proteins of RSV was investigated using Bimolecular fluorescence complementation(BiFC),and the interaction between NSvc4 and CP was further confirmed by yeast two-hybrid experiments.The possible involvement of NSvc4 and CP in pathogenicity was exploited by expressing them individually or together in N.benthamiana employing the vector Potato virus X(PVX).【Result】 NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed,both NSvc4 and CP could induce leaf mosaic symptoms when they were expressed using PVX.However,leaf mosaic symptoms caused by CP was recovered in systemic leaves of PVX inoculated plants.When expressed together or as a fused protein using PVX,NSvc4 and CP induced severe symptoms in both inoculated and systemic leaves of N.benthamiana.Real-time PCR experiments showed that NSvc4 and CP had no effects on the accumulation of PVX,and the severity of the symptoms induced by the recombinant virus correlated with the expression levels of NSvc4 and CP.【Conclusion】 Both NSvc4 and CP have pathogenicity properties and they might have synergistical function in RSV pathogenesis.
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Claims 9
Claim · model-written summary
NSvc4 interacted with CP and formed complexes in chloroplasts of Nicotiana benthamiana.
- Taxon
- N.benthamiana
Nicotiana benthamianae0 - Organ / stage
- chloroplastse0
- Interactor
- Rice stripe virus(RSV)t1pathogen
- Gene
- NSvc4e0
- Outcome
- protein complexese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
t0“Rice Stripe Virus”
t1“Rice stripe virus(RSV)”
Claim · model-written summary
NSvc4 expressed using PVX induced leaf mosaic symptoms.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Treatment
- NSvc4e0
- Interactor
- Rice stripe virus(RSV)t1pathogen
- Gene
- NSvc4e0
- Outcome
- leaf mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both NSvc4 and CP could induce leaf mosaic symptoms when they were expressed using PVX”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
t1“Rice stripe virus(RSV)”
Claim · model-written summary
CP expressed using PVX induced leaf mosaic symptoms.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Treatment
- CPe0
- Interactor
- Rice stripe virus(RSV)t1pathogen
- Gene
- CPe0
- Outcome
- leaf mosaic symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both NSvc4 and CP could induce leaf mosaic symptoms when they were expressed using PVX”
t1“Rice stripe virus(RSV)”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
Claim · model-written summary
CP-caused leaf mosaic symptoms recovered in systemic leaves of PVX-inoculated plants.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Organ / stage
- systemic leavese0
- Interactor
- Potato virus X(PVX)Potato virus Xe2pathogen
- Gene
- CPe0
- Outcome
- leaf mosaic symptoms caused by CP was recoverede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leaf mosaic symptoms caused by CP was recovered in systemic leaves of PVX inoculated plants”
t1“Rice stripe virus(RSV)”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
e2“Potato virus X(PVX)”
Claim · model-written summary
Coexpression of NSvc4 and CP using PVX caused severe symptoms in inoculated and systemic leaves.
- Taxon
- N.benthamianaNicotiana benthamianae0
- Treatment
- expressed togethere0
- Interactor
- Potato virus X(PVX)Potato virus Xe2pathogen
- Gene
- NSvc4 and CPe0
- Outcome
- severe symptoms in both inoculated and systemic leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When expressed together or as a fused protein using PVX,NSvc4 and CP induced severe symptoms in both inoculated and systemic leaves of N.benthamiana”
e2“Potato virus X(PVX)”
Claim · model-written summary
Fused NSvc4 and CP expressed using PVX caused severe symptoms in inoculated and systemic leaves.
- Taxon
- N.benthamianaNicotiana benthamianae0
- Treatment
- as a fused protein using PVXe0
- Interactor
- Potato virus X(PVX)Potato virus Xe2pathogen
- Gene
- NSvc4 and CPe0
- Outcome
- severe symptoms in both inoculated and systemic leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When expressed together or as a fused protein using PVX,NSvc4 and CP induced severe symptoms in both inoculated and systemic leaves of N.benthamiana”
e2“Potato virus X(PVX)”
Claim · model-written summary
NSvc4 and CP did not affect PVX accumulation.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Treatment
- NSvc4 and CPe0
- Interactor
- Potato virus X(PVX)Potato virus Xe2pathogen
- Gene
- NSvc4 and CPe0
- Outcome
- accumulation of PVXe0
- Direction
- no effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NSvc4 and CP had no effects on the accumulation of PVX”
t1“Rice stripe virus(RSV)”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
e2“Potato virus X(PVX)”
Claim · model-written summary
Symptom severity from the recombinant virus correlated with NSvc4 and CP expression levels.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Interactor
- Potato virus X(PVX)Potato virus Xe2pathogen
- Gene
- NSvc4 and CPe0
- Outcome
- severity of the symptoms induced by the recombinant viruse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the severity of the symptoms induced by the recombinant virus correlated with the expression levels of NSvc4 and CP”
t1“Rice stripe virus(RSV)”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
e2“Potato virus X(PVX)”
Claim · model-written summary
The authors infer that NSvc4 and CP might act synergistically in RSV pathogenesis.
- Taxon
- N.benthamianaNicotiana benthamianae1
- Interactor
- Rice stripe virus(RSV)t1pathogen
- Gene
- NSvc4 and CPe0
- Outcome
- synergistical function in RSV pathogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both NSvc4 and CP have pathogenicity properties and they might have synergistical function in RSV pathogenesis”
t1“Rice stripe virus(RSV)”
e1“NSvc4 interacted with CP and they formed protein complexes in chloroplasts of N.benthamiana when co-expressed”
The same questions in other papers
Solanum tuberosum · Potato virus X · Nicotiana benthamianaPositive and negative results
22 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.