Abstract
【Objective】The aim of this study was to isolate the AP1 homologous gene from strawberry(Fragaria×ananassa),analyze its expression level in different tissues,organs and at different growth stages of flowers and elucidate the role of it in the floral development progress of strawberry【.Method】The fragment of an AP1 homologous gene was amplified from young leaves and flower buds of strawberry with the degenerate primers designed according to the conserved sequences of AP1 homologous genes in the other species.The full-length cDNA sequence of this gene was obtained with RACE technique.Real time RT-PCR was used to analyze the expression patterns of this gene in different tissues,organs and at different growth stages of flowering of strawberry.A promoter sequence was isolated with chromosome walking methods.【Result】 An AP1 homologous gene in strawberry was cloned from cultivar ‘Huaji’ and it was designated as FaAP1.The CDS length of FaAP1 is 735 bp and encodes a predicted protein of 245 amino acid residues.Its amino acid sequence shows a highest homology with the AP1-1 of Rosa,up to 92%,and also has a homology of 64.00% when compared with the AtAP1 of Arabidopsis thaliana.FaAP1 is a MIKC type MADS-box gene for its amino acid sequence contained the typical domains of MADS-box,I-region,K-box and C-terminal.The results of real time RT-PCR showed that the expression patterns of FaAP1 changed in different tissues,organs and flowers of different growth stages.In addition to the TATA/CAAT-box,its promoter also contained some specific regulatory elements【.Conclusion】FaAP1,an AP1 homologous gene in strawberry,was isolated and it may play an important role in the progress of flowering in strawberry.
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Claims 9
Claim · model-written summary
An AP1 homologous gene, FaAP1, was cloned from strawberry cultivar Huaji.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Material
- ‘Huaji’e0
- Gene
- FaAP1e0
- Outcome
- was clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“An AP1 homologous gene in strawberry was cloned from cultivar ‘Huaji’ and it was designated as FaAP1”
Claim · model-written summary
The FaAP1 coding sequence is 735 bp.
Not reportedTaxon
Verbatim
e0“The CDS length of FaAP1 is 735 bp”
Claim · model-written summary
FaAP1 encodes a predicted 245-amino-acid protein.
Not reportedTaxon
Verbatim
e0“encodes a predicted protein of 245 amino acid residues”
Claim · model-written summary
FaAP1 had 92% amino-acid homology with Rosa AP1-1.
Not reportedTaxon
Verbatim
e0“Its amino acid sequence shows a highest homology with the AP1-1 of Rosa,up to 92%”
Claim · model-written summary
FaAP1 had 64.00% homology with Arabidopsis AtAP1.
Not reportedTaxon
Verbatim
e0“homology of 64.00% when compared with the AtAP1 of Arabidopsis thaliana”
Claim · model-written summary
FaAP1 was classified as a MIKC type MADS-box gene with characteristic domains.
Not reportedTaxon
Verbatim
e0“FaAP1 is a MIKC type MADS-box gene for its amino acid sequence contained the typical domains of MADS-box,I-region,K-box and C-terminal”
Claim · model-written summary
FaAP1 expression varied among tissues, organs and flower growth stages.
Not reportedTaxon
Verbatim
e0“expression patterns of FaAP1 changed in different tissues,organs and flowers of different growth stages”
Claim · model-written summary
The FaAP1 promoter contained TATA/CAAT-box and other specific regulatory elements.
- Outcome
- promoter also contained some specific regulatory elementse0
Not reportedTaxon
Verbatim
e0“In addition to the TATA/CAAT-box,its promoter also contained some specific regulatory elements”
Claim · model-written summary
FaAP1 may play an important role in strawberry flowering.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaAP1e0
- Outcome
- progress of floweringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaAP1,an AP1 homologous gene in strawberry,was isolated and it may play an important role in the progress of flowering in strawberry”