Abstract
[Objective] The aim of the study was to observe the relationship between drought stress and programmed cell death with DAPI and search for a good morphology way to observe programmed cell death.[Method] Two Malus plants(Malus robusta Rehd and Malus hupehensis pamp Reld.seedlings) were treated with twenty percent of polythyene glycol(20%PEG6000) and DAPI UV to stimulate the treated roots.[Result]The study showed that after DAPI staining by ultraviolet(UV) radiation,the normal issue nuclei of new roots issued uniform blue fluorescence.The features of programmed cell death(PCD) were found in the treated roots cell,such as chromatin condensated,karyon shinked,distorted and degraduated with rising of stress time.[Conclusion] Drought stress could induce programmed cell death in Malus plants,and DAPI was a good way to identify programmed cell death.
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Claims 4
Claim · model-written summary
Normal new root nuclei fluoresced uniformly blue after DAPI staining.
MalusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after DAPI staining by ultraviolet(UV) radiation,the normal issue nuclei of new roots issued uniform blue fluorescence”
t0“Malus”
Claim · model-written summary
Drought treated root cells showed chromatin condensation, nuclear shrinkage, distortion and degradation over time.
- Organ / stage
- treated roots celle0
- Treatment
- twenty percent of polythyene glycol(20%PEG6000)e1
- Outcome
- chromatin condensated,karyon shinked,distorted and degraduatede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The features of programmed cell death(PCD) were found in the treated roots cell,such as chromatin condensated,karyon shinked,distorted and degraduated with rising of stress time”
e1“twenty percent of polythyene glycol(20%PEG6000)”
t0“Malus”
Claim · model-written summary
Drought stress induced programmed cell death in Malus.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Drought stress could induce programmed cell death in Malus plants”
Claim · model-written summary
DAPI identified programmed cell death effectively.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DAPI was a good way to identify programmed cell death”
t0“Malus”