Abstract
Plant hormone brassinosteroid(BR)can improve the drought resistance of plant.This study explores the effects of spraying BR on the expression of transcription factor BES1/BRI1 in cotton seedling under drought stress.It is helpful for elucidating the mechanism of BR improving the drought resistance of cotton.The BES1/BZR1 gene was cloned by using PCR technique from the seedling of cotton‘XLZ17'(Gossypium hirsutum)and named as GhBES1/BZR1(GenBank accession number KP272000).Sequence analysis indicated that GhBES1/BZR1 contains an open reading frame(ORF)of 960 bp,which encodes 319 amino acids included a conserved domain DUF822.The calculated molecular weight is 34.3kD and the theoretical isoelectric point is 8.95.After 2.5% PEG-6000 treatment for 24 h,water,BR(brassinosteroids)and Z(BR inhibitor)were sprayed on the seedlings of cotton‘XLZ17',respectively.The effects of spraying BR on the expression of GhBES1/BZR1 were analyzed by the real time quantitative PCR(qRT-PCR).The results showed that the expression levels of GhBES1/BZR1 were greatly increased after BR spraying within 3h,and then the expression levels of GhBES1/BZR1 were significantly decreased after 6and 12 h.Theresults demonstrated that spraying BR quickly up-regulated the expression of GhBES1/BZR1 under drought stress,which might be contributed drought resistance of cotton.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 4
Claim · model-written summary
GhBES1/BZR1 contains an open reading frame of 960 bp encoding 319 amino acids
- Taxon
- Cotton
Gossypium hirsutumt0 - Gene
- GhBES1/BZR1e1
- Outcome
- open reading framee1
- Value
- 960 bpe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcription Factor BES1/BRI1 of Cotton Seedling”
e1“GhBES1/BZR1 contains an open reading frame(ORF)of 960 bp,which encodes 319 amino acids”
Claim · model-written summary
Spraying BR greatly increased GhBES1/BZR1 expression in cotton seedlings within 3 h of drought treatment
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- seedlingse6
- Treatment
- BR sprayinge3
- Gene
- GhBES1/BZR1e3
- Outcome
- expression levels of GhBES1/BZR1e3
- Direction
- greatly increasede3
- Qualifier
- within 3he3
- Comparator
- watere6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcription Factor BES1/BRI1 of Cotton Seedling”
e3“the expression levels of GhBES1/BZR1 were greatly increased after BR spraying within 3h”
e6“After 2.5% PEG-6000 treatment for 24 h, water, BR(brassinosteroids) and Z(BR inhibitor) were sprayed on the seedlings of cotton”
Claim · model-written summary
GhBES1/BZR1 expression was significantly decreased after 6 and 12 h following BR spraying
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- BRe6
- Gene
- GhBES1/BZR1e4
- Outcome
- expression levels of GhBES1/BZR1e4
- Direction
- significantly decreasede4
- Qualifier
- after 6and 12 he4
- Comparator
- watere6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcription Factor BES1/BRI1 of Cotton Seedling”
e4“the expression levels of GhBES1/BZR1 were significantly decreased after 6and 12 h”
e6“After 2.5% PEG-6000 treatment for 24 h, water, BR(brassinosteroids) and Z(BR inhibitor) were sprayed on the seedlings of cotton”
Claim · model-written summary
Spraying BR may contribute to drought resistance of cotton through up-regulation of GhBES1/BZR1
- Taxon
- cottonGossypium hirsutume5
- Treatment
- spraying BRe5
- Gene
- GhBES1/BZR1e5
- Outcome
- drought resistancee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Transcription Factor BES1/BRI1 of Cotton Seedling”
e5“spraying BR quickly up-regulated the expression of GhBES1/BZR1 under drought stress,which might be contributed drought resistance of cotton”
The same questions in other papers
brassinolide · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →application · molecular: expression · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.