Abstract
In the experiment,a soybean suspension cells was initially established with the callus induced from the hypocotyl of sterile seedling of Jilinxiaoli 7 soybean. And then the influence of concentrations of boric acid on the growth of suspension cell cultures( fresh weight) were determinated and the transformation of exogenous GUS gene to the cells were conducted by using Agrobacterium-mediated method. The transformation efficiencies influenced by sonication duration and boric acid concentration were mainly investigated through histochemical method in order to find an appropriate genetic transformation way. The positive transformed cell cultures were further identified by PCR technique and Southern blot hybridization. The results showed that boric acid concentration for normal growth of soybean suspension cell was between 2. 0 and 10 mg · L-1,higher or lower boron medium would inhibited soybean cell growth,especially at a high level of more than 100 mg · L-1. However,the appropriate increase in boric acid concentration( 10-30 mg · L-1) could improve the transformation efficiency of the cell suspension,which was also helpful to the screening of the transformant in the transformation process. Twenty eight transformants were obtained in 1 mg transformed cells under 30 mg·L-1boric acid concentration with the assistance of 3 min sonication.
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Claims 4
Claim · model-written summary
Soybean suspension cells grew normally at boric acid concentrations from 2.0 to 10 mg L-1.
- Taxon
- soybean
Glycine maxe0 - Treatment
- boric acid concentratione0
- Outcome
- normal growth of soybean suspension celle0
- Value
- between 2. 0 and 10 mg · L-1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“boric acid concentration for normal growth of soybean suspension cell was between 2. 0 and 10 mg · L-1”
t0“Soybean”
Claim · model-written summary
Boron concentrations outside that range inhibited soybean cell growth, especially above 100 mg L-1.
- Taxon
- soybeanGlycine maxe0
- Treatment
- higher or lower boron mediume0
- Outcome
- soybean cell growthe0
- Direction
- inhibitede0
- Qualifier
- more than 100 mg · L-1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher or lower boron medium would inhibited soybean cell growth,especially at a high level of more than 100 mg · L-1”
t0“Soybean”
Claim · model-written summary
Increasing boric acid to 10–30 mg L-1 improved soybean suspension-cell transformation efficiency and transformant screening.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- boric acid concentration( 10-30 mg · L-1)e0
- Outcome
- transformation efficiency of the cell suspensione0
- Direction
- improvee0
- Value
- 10-30 mg · L-1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“appropriate increase in boric acid concentration( 10-30 mg · L-1) could improve the transformation efficiency of the cell suspension,which was also helpful to the screening of the transformant”
t0“Soybean”
Claim · model-written summary
At 30 mg L-1 boric acid with 3 minutes of sonication, 28 transformants per 1 mg transformed cells were obtained.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 30 mg·L-1boric acid concentration with the assistance of 3 min sonicatione0
- Outcome
- transformantse0
- Value
- Twenty eight transformantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty eight transformants were obtained in 1 mg transformed cells under 30 mg·L-1boric acid concentration with the assistance of 3 min sonication.”
t0“Soybean”
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