Abstract
Vicia faba protoplasts were enzymatically isolated from the immature cotyledons, with a high protoplasts yields (3.3×10~7g~(-1)FW)after protoplast purifications, and initially cultured in a modified K_(8p) liquid medium supplemented with 0. 2 mg/L 2, 4-D, 1 mg/L NAA and 0. 5 mg/L BA. Higher density(7. 5×10~5/ml) in the initial culture of protoplasts is favourable to the cell wall regeneration and cell division of cultured immature cotyledon protoplasts among the densities tested. The division frequency of protoplast-derived cells counted at 12 days reaches 44. 8％. After 12 days of culture the addition of K_8 fresh medium containing 5 mg/L each of ascorbic acid and PVP was essential to sustained cell division, colony proliferation and could avoid cell browning. After 6 weeks,the cell colonies formed and transferred to the gelrite-solidfied K_8 cell medium with 0. 1～0. 2 mg/L NAA and 0.5～1.0 mg/L BA which supported the further proliferation and callus growth. When calli of 2～3 mm in diameter were subcultured on MSB growth medium with 0. 2 mg/L NAA and 0. 5 mg/L BA,approxirnately 25％ of them developed into compact and nodular calli. Shoot buds were regenerated on the surface of the nodular calli with a frequency of 17％, when the calli were placed on MSB differetiation medium with 0. 25 mg/L IAA and 1.0 mg/L each of BA and Zeatin. Whole plants were regenerated upon the transfer of 3～4 cm shoots to 1/2 MS medium with 0. 5 mg/L IBA, thirty-six complete plants were obtained, and fifteen of them transplanted into the pots are growing well in the phytotron.
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Claims 9
Claim · model-written summary
Protoplasts isolated from immature Vicia faba cotyledons gave a high yield.
- Taxon
- Vicia faba
Vicia fabae0 - Organ / stage
- immature cotyledonse0
- Outcome
- protoplasts yieldse1
- Value
- 3.3×10~7g~(-1)FWe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e1“with a high protoplasts yields (3.3×10~7g~(-1)FW)after protoplast purifications”
Claim · model-written summary
A higher protoplast density of 7.5×10^5/ml was favourable to cell wall regeneration and cell division among the densities tested.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- Higher densitye2
- Outcome
- cell wall regeneration and cell divisione2
- Direction
- favourablee2
- Value
- 7. 5×10~5/mle2
- Comparator
- among the densities testede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e2“Higher density(7. 5×10~5/ml) in the initial culture of protoplasts is favourable to the cell wall regeneration and cell division”
e3“among the densities tested”
Claim · model-written summary
The division frequency of protoplast-derived cells reached 44.8％ at 12 days.
- Taxon
- Vicia fabaVicia fabae0
- Outcome
- division frequency of protoplast-derived cellse4
- Value
- 44. 8％e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e4“The division frequency of protoplast-derived cells counted at 12 days reaches 44. 8％”
Claim · model-written summary
Adding ascorbic acid and PVP to fresh medium was essential to sustained cell division.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- K_8 fresh medium containing 5 mg/L each of ascorbic acid and PVPe5
- Outcome
- sustained cell divisione5
- Direction
- essentiale5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e5“the addition of K_8 fresh medium containing 5 mg/L each of ascorbic acid and PVP was essential to sustained cell division”
Claim · model-written summary
Adding ascorbic acid and PVP to fresh medium could avoid cell browning.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- K_8 fresh medium containing 5 mg/L each of ascorbic acid and PVPe5
- Outcome
- cell browninge6
- Direction
- could avoide6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e5“the addition of K_8 fresh medium containing 5 mg/L each of ascorbic acid and PVP was essential to sustained cell division”
e6“could avoid cell browning”
Claim · model-written summary
About 25％ of the calli developed into compact and nodular calli.
- Taxon
- Vicia fabaVicia fabae0
- Organ / stage
- callie7
- Outcome
- compact and nodular callie7
- Value
- 25％e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e7“approxirnately 25％ of them developed into compact and nodular calli”
Claim · model-written summary
Shoot buds regenerated on nodular calli at a frequency of 17％ on MSB differentiation medium.
- Taxon
- Vicia fabaVicia fabae0
- Organ / stage
- nodular callie1
- Treatment
- MSB differetiation medium with 0. 25 mg/L IAA and 1.0 mg/L each of BA and Zeatine2
- Outcome
- Shoot budse1
- Value
- 17％e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e1“Shoot buds were regenerated on the surface of the nodular calli with a frequency of 17％”
e2“when the calli were placed on MSB differetiation medium with 0. 25 mg/L IAA and 1.0 mg/L each of BA and Zeatin”
Claim · model-written summary
Thirty-six complete Vicia faba plants were obtained.
- Taxon
- Vicia fabaVicia fabae0
- Outcome
- complete plantse1
- Value
- thirty-sixe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e1“thirty-six complete plants were obtained”
Claim · model-written summary
Fifteen regenerated plants transplanted into pots were growing well in the phytotron.
- Taxon
- Vicia fabaVicia fabae0
- Outcome
- growing well in the phytotrone1
- Value
- fifteene1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Vicia faba protoplasts were enzymatically isolated from the immature cotyledons”
e1“fifteen of them transplanted into the pots are growing well in the phytotron”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.